Pub Date : 2024-07-01DOI: 10.21608/ejah.2024.365992
S. M. Mansour, Rehab E. M. Gaafar, Walaa A. El - Shaer
V ibrios are the most widespread species of all crustaceans, including shrimp, which are susceptible. Numerous Vibrio species are linked to food - borne illnesses and are harmful to humans. The present investigation was performed to identify the prevalence and virulence genes of Vibrio species isolated from white shrimp (Metapenaeus stebbingi) and test the efficacy of some organic acids on its survival with improving shrimp shelf life. Samples of white shrimp (M. stebbingi) were collected from fisher-men in Ismailia City, Egypt, between July and November 2023. Vibrio species were isolated and identified from the shrimp's hepatopancreas and musculature using its specific media (Thiosulfate - Citrate - Bile - Sucrose Agar) and biochemical tests. Then, the identification was confirmed and their virulence genes were detected by using PCR. Moreover, trials were performed by using acetic and citric acids treatments for decreasing Vibrio parahaemolyticus counts in artificially inoculated shrimp at different treatment durations, as well as for extending shelf life of chilled shrimp at refrigeration storage (4 o C). The sensory attributes, pH values, total aerobic plate counts and lipid oxidation were evaluated under refrigeration at interval 0, 3, 6, 9 and 12 days. Results revealed that Vibrio parahaemolyticus and Vibrio alginolyticus were found to be present in naturally infected white shrimp (M. stebbingi), with a total prevalence of 14%, where V. alginolyticus was the most prevalent, accounting for 9.6 ,%
{"title":"Insights on Vibriosis in white shrimp (Metapenaeus stebbingi): prevalence, virulence genes, and potential limitations of Existence in the meat","authors":"S. M. Mansour, Rehab E. M. Gaafar, Walaa A. El - Shaer","doi":"10.21608/ejah.2024.365992","DOIUrl":"https://doi.org/10.21608/ejah.2024.365992","url":null,"abstract":"V ibrios are the most widespread species of all crustaceans, including shrimp, which are susceptible. Numerous Vibrio species are linked to food - borne illnesses and are harmful to humans. The present investigation was performed to identify the prevalence and virulence genes of Vibrio species isolated from white shrimp (Metapenaeus stebbingi) and test the efficacy of some organic acids on its survival with improving shrimp shelf life. Samples of white shrimp (M. stebbingi) were collected from fisher-men in Ismailia City, Egypt, between July and November 2023. Vibrio species were isolated and identified from the shrimp's hepatopancreas and musculature using its specific media (Thiosulfate - Citrate - Bile - Sucrose Agar) and biochemical tests. Then, the identification was confirmed and their virulence genes were detected by using PCR. Moreover, trials were performed by using acetic and citric acids treatments for decreasing Vibrio parahaemolyticus counts in artificially inoculated shrimp at different treatment durations, as well as for extending shelf life of chilled shrimp at refrigeration storage (4 o C). The sensory attributes, pH values, total aerobic plate counts and lipid oxidation were evaluated under refrigeration at interval 0, 3, 6, 9 and 12 days. Results revealed that Vibrio parahaemolyticus and Vibrio alginolyticus were found to be present in naturally infected white shrimp (M. stebbingi), with a total prevalence of 14%, where V. alginolyticus was the most prevalent, accounting for 9.6 ,%","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"9 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141701622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.21608/ejah.2024.353379
Biochemistry Toxicology Marwa F. Hassan, Feed Deficiency
T his study was conducted to determine bovine mastitis resistance genes of common etiological agents in Al-Sharkia province. In addition to monitor the changes in antioxidants profile, oxidative stress status and some of biochemical markers in dairy cows suffered from clinical mastitis. It was carried out on forty dairy cattle (40) aged from 4 – 6 years (20 healthy and 20 mastitic) from dairy farms and individual cases in Al-Sharkia Governorate, dependent on the findings of the clinical examination, the cattle were divided into 3 groups. Gp1 healthy dairy cattle (control), Gp2 mastitic dairy cattle before treatment, Gp3 mastitic dairy cattle after treatment. The clinical symptoms of mastitis including milk and udder were reported in Gp2. Within the bacterial isolates (20) it was noticed that the highest spread was of Staphylococcus aureus (10) followed by E.coli (6) and Klebsiella Spp. (4). Antibiotic sensitivity test displayed that gentamycin, streptomycin
{"title":"Overview on the mastitis syndrome in cattle in Al-Sharkia governorate","authors":"Biochemistry Toxicology Marwa F. Hassan, Feed Deficiency","doi":"10.21608/ejah.2024.353379","DOIUrl":"https://doi.org/10.21608/ejah.2024.353379","url":null,"abstract":"T his study was conducted to determine bovine mastitis resistance genes of common etiological agents in Al-Sharkia province. In addition to monitor the changes in antioxidants profile, oxidative stress status and some of biochemical markers in dairy cows suffered from clinical mastitis. It was carried out on forty dairy cattle (40) aged from 4 – 6 years (20 healthy and 20 mastitic) from dairy farms and individual cases in Al-Sharkia Governorate, dependent on the findings of the clinical examination, the cattle were divided into 3 groups. Gp1 healthy dairy cattle (control), Gp2 mastitic dairy cattle before treatment, Gp3 mastitic dairy cattle after treatment. The clinical symptoms of mastitis including milk and udder were reported in Gp2. Within the bacterial isolates (20) it was noticed that the highest spread was of Staphylococcus aureus (10) followed by E.coli (6) and Klebsiella Spp. (4). Antibiotic sensitivity test displayed that gentamycin, streptomycin","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"389 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141707939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.21608/ejah.2024.359058
{"title":"The role of ionized water as a safe alternative to disinfectants in poultry Slaughter houses","authors":"","doi":"10.21608/ejah.2024.359058","DOIUrl":"https://doi.org/10.21608/ejah.2024.359058","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"17 43","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141700055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.21608/ejah.2024.367392
Fawzia A. El - Shenawy, S. E. Zahran, Tamer M.H. El, Sharawey, Asmaa T. Talayea
.
.
{"title":"Anti-activity of Sodium Bicarbonate on Biofilm Formation by some bacterial isolates from raw milk","authors":"Fawzia A. El - Shenawy, S. E. Zahran, Tamer M.H. El, Sharawey, Asmaa T. Talayea","doi":"10.21608/ejah.2024.367392","DOIUrl":"https://doi.org/10.21608/ejah.2024.367392","url":null,"abstract":".","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"761 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141851899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.21608/ejah.2024.353391
Biochemistry Lab Haidy E. Mohamed
I n Egypt, the practice of raising chicken in farms as well as backyard is widespread. Ochratoxin A (OTA) is one of the most common mycotox-ins in chicken feed and their meat, which poses a concern for both animal and human health. The study aimed to investigate OTA residues by us-ing ELISA technique in meat, liver and kidney samples. Additionally, it aimed to evaluate total protein, albumin, globulin, functions and antioxidant state of both liver and kidney, in addition to assess some meat quality parameters of both farm-raised and backyard chicken. A total of 60 chicken (30 each of farm-raised and backyard chickens) were collected across different regions of Ismailia City, Egypt. The results revealed that incidence of OTA residues was significantly higher in all farm-raised chicken samples than in all backyard chicken samples, with significant higher concentration in kidney samples, followed by liver samples then breast and thigh meat samples. According to the Egyptian and International Standards, neither breast and thigh meat nor liver samples exceeded the permissible limits in both breeding systems. Moreover, farm raised chicken revealed noteworthy decline (P<0.05) in serum total proteins and albumin. Conversely, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine and urea exhibited significant increases. A significant reduction in total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPx) within kidney and liver. Furthermore, there were no significant differences between backyard and farmed raised chicken meat in pH values, moisture%, protein% and ash %. However, significantly higher fat % and thiobarbeturic acid reactive substances (TBARS) content in farm raised chicken meat than backyard ones. In conclusion, OTA residues were higher in farm-raised chicken, inversely affecting their biochemical and oxidative capacity with no differences in meat quality except their higher fat content and higher meat oxidation. Regular monitoring for OTA residues in chickens and their feed with effective management and control strategies are essential for all breeding systems
在埃及，农场和后院养鸡的做法十分普遍。赭曲霉毒素 A（OTA）是鸡饲料和鸡肉中最常见的霉菌毒素之一，对动物和人类健康都造成了威胁。这项研究旨在利用酶联免疫吸附技术，调查肉类、肝脏和肾脏样本中的赭曲霉毒素 A 残留量。此外，研究还旨在评估农场饲养鸡和散养鸡的总蛋白、白蛋白、球蛋白、肝脏和肾脏的功能和抗氧化状态，以及一些肉质参数。研究人员在埃及伊斯梅利亚市的不同地区共收集了 60 只鸡（农场饲养鸡和散养鸡各 30 只）。结果显示，所有农场饲养的鸡肉样本中 OTA 残留的发生率明显高于所有散养鸡样本，其中肾脏样本中的浓度明显较高，其次是肝脏样本，然后是胸肉和大腿肉样本。根据埃及标准和国际标准，这两种饲养系统中的胸肉和大腿肉样本以及肝脏样本均未超过允许限值。此外，农场饲养鸡的血清总蛋白和白蛋白显著下降（P<0.05）。相反，血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、肌酐和尿素则显著增加。肾脏和肝脏的总抗氧化能力（TAC）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）明显下降。此外，散养鸡肉和养殖鸡肉在 pH 值、水分百分比、蛋白质百分比和灰分百分比方面没有明显差异。不过，农场饲养鸡肉的脂肪率和硫代巴比妥酸活性物质（TBARS）含量明显高于散养鸡肉。总之，农场饲养的鸡肉中 OTA 残留量较高，会对其生化和氧化能力产生负面影响，但除了脂肪含量较高和肉的氧化程度较高外，肉质并无差异。定期监测鸡及其饲料中的 OTA 残留，并采取有效的管理和控制策略，对所有养殖系统都至关重要。
{"title":"Assessment of Ochratoxin (OTA) residues in meat and their biochemical effects in commercial farmed and backyard chicken","authors":"Biochemistry Lab Haidy E. Mohamed","doi":"10.21608/ejah.2024.353391","DOIUrl":"https://doi.org/10.21608/ejah.2024.353391","url":null,"abstract":"I n Egypt, the practice of raising chicken in farms as well as backyard is widespread. Ochratoxin A (OTA) is one of the most common mycotox-ins in chicken feed and their meat, which poses a concern for both animal and human health. The study aimed to investigate OTA residues by us-ing ELISA technique in meat, liver and kidney samples. Additionally, it aimed to evaluate total protein, albumin, globulin, functions and antioxidant state of both liver and kidney, in addition to assess some meat quality parameters of both farm-raised and backyard chicken. A total of 60 chicken (30 each of farm-raised and backyard chickens) were collected across different regions of Ismailia City, Egypt. The results revealed that incidence of OTA residues was significantly higher in all farm-raised chicken samples than in all backyard chicken samples, with significant higher concentration in kidney samples, followed by liver samples then breast and thigh meat samples. According to the Egyptian and International Standards, neither breast and thigh meat nor liver samples exceeded the permissible limits in both breeding systems. Moreover, farm raised chicken revealed noteworthy decline (P<0.05) in serum total proteins and albumin. Conversely, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine and urea exhibited significant increases. A significant reduction in total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPx) within kidney and liver. Furthermore, there were no significant differences between backyard and farmed raised chicken meat in pH values, moisture%, protein% and ash %. However, significantly higher fat % and thiobarbeturic acid reactive substances (TBARS) content in farm raised chicken meat than backyard ones. In conclusion, OTA residues were higher in farm-raised chicken, inversely affecting their biochemical and oxidative capacity with no differences in meat quality except their higher fat content and higher meat oxidation. Regular monitoring for OTA residues in chickens and their feed with effective management and control strategies are essential for all breeding systems","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"17 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141704208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.21608/ejah.2024.358432
W. Baher, Marwa Ezzat, Elkenawy Mansour, Millisa Royas Wormely, Eman Mahmoud, Abdelaziz Elmehrath, Arwa Hassan, Mohamed Nassar, W. Darwish
T he purpose of this study was to look at the likelihood of mold contamination in salted feseikh, sardine, and smoked herrings that are marketed in retail settings. Additionally, the total aflatoxins (AFTs) in the examined samples were estimated and further discussion was held regarding their potential health risks associated with them. The effect of the electro-lyzed water (EW) on the residual concentrations of AFTs and the overall amount of mold in the salted feseikh was also investigated. Feseikh had the highest mold contamination, followed by salted sardine and smoked herrings at 80%, 50%, and 23.33%, respectively. Total mold count was 3.05±0.15 log 10 cfu/g in feseikh, followed by salted sardine (2.68±0.09), and smoked her-rings (2.26±0.04), respectively. Four mold genera were recovered from the examined samples under examination: Aspergillus spp., Penicillium spp., Cladosporium spp ., and Fusarium spp . The most predominant mold species found in the analyzed samples was Aspergillus spp . The analyzed samples had recorded mean values of total aflatoxins (ppb) of 3.98±0.27 (feseikh), 3.19±0.25 (salted sardine
{"title":"Analysis of mold and aflatoxins in processed fish and their control with electrolyzed water","authors":"W. Baher, Marwa Ezzat, Elkenawy Mansour, Millisa Royas Wormely, Eman Mahmoud, Abdelaziz Elmehrath, Arwa Hassan, Mohamed Nassar, W. Darwish","doi":"10.21608/ejah.2024.358432","DOIUrl":"https://doi.org/10.21608/ejah.2024.358432","url":null,"abstract":"T he purpose of this study was to look at the likelihood of mold contamination in salted feseikh, sardine, and smoked herrings that are marketed in retail settings. Additionally, the total aflatoxins (AFTs) in the examined samples were estimated and further discussion was held regarding their potential health risks associated with them. The effect of the electro-lyzed water (EW) on the residual concentrations of AFTs and the overall amount of mold in the salted feseikh was also investigated. Feseikh had the highest mold contamination, followed by salted sardine and smoked herrings at 80%, 50%, and 23.33%, respectively. Total mold count was 3.05±0.15 log 10 cfu/g in feseikh, followed by salted sardine (2.68±0.09), and smoked her-rings (2.26±0.04), respectively. Four mold genera were recovered from the examined samples under examination: Aspergillus spp., Penicillium spp., Cladosporium spp ., and Fusarium spp . The most predominant mold species found in the analyzed samples was Aspergillus spp . The analyzed samples had recorded mean values of total aflatoxins (ppb) of 3.98±0.27 (feseikh), 3.19±0.25 (salted sardine","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141706023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.21608/ejah.2024.336965
{"title":"Molecular Characterization of antimicrobial resistant Escherichia coli and Salmonella species isolated from retail chicken with control trial using organic acids in vitro.","authors":"","doi":"10.21608/ejah.2024.336965","DOIUrl":"https://doi.org/10.21608/ejah.2024.336965","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"11 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140353918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.21608/ejah.2024.346750
Eman M. El, El-Sherbeny
M ycotoxins, a global challenge, represent one of the most significant hazards that affect foods and feeds. It produced naturally as secondary metabolites by various species of toxigenic fungi. It can cause chronic or acute toxicity due to their immunosuppressive, carcinogenic and mutagenic properties in animals and human. Every year, mycotoxins cause massive economic losses in the animal feed sector and animal hus-bandry. Human affected by mycotoxins either indirectly through consumption of contaminated animal products (meat, eggs and milk) by mycotoxins' residue or directly through consumption of contaminated foods (nuts, coffee, corn, barley, wheat, peanuts, peas) and their by-products. This review gives an overview of the most important and prevalent mycotoxins in animal feeds, health and economic mycotoxins impacts on animals. In addition, the main conventional and advanced approaches in mycotoxins analytical detection techniques and decontamination strategies to mitigate and counteract mycotoxin contamination of feedstuffs were also reported. There are different analytical techniques to precisely qualities and quantities mycotoxins. They included Fluorometer, chromatography-based devices and immunological based techniques besides other recent advanced techniques. Various my-cotoxins detoxification strategies have been developed included physical, chemical and biological strategies to reduce or eliminate mycotoxins in feed ingredients or complete compound feeds, however they cannot totally de-contaminate mycotoxins. Hence, they varied in their limitations or abilities to meet the requirements of practical application according to many factors including their binding efficiency, environmental protection, feeds and foods safety, palatability or cost-effectiveness.
{"title":"Mycotoxins: Review on types, toxicity, conventional and updating techniques of detection and counteraction in feeds and foods of animals and human","authors":"Eman M. El, El-Sherbeny","doi":"10.21608/ejah.2024.346750","DOIUrl":"https://doi.org/10.21608/ejah.2024.346750","url":null,"abstract":"M ycotoxins, a global challenge, represent one of the most significant hazards that affect foods and feeds. It produced naturally as secondary metabolites by various species of toxigenic fungi. It can cause chronic or acute toxicity due to their immunosuppressive, carcinogenic and mutagenic properties in animals and human. Every year, mycotoxins cause massive economic losses in the animal feed sector and animal hus-bandry. Human affected by mycotoxins either indirectly through consumption of contaminated animal products (meat, eggs and milk) by mycotoxins' residue or directly through consumption of contaminated foods (nuts, coffee, corn, barley, wheat, peanuts, peas) and their by-products. This review gives an overview of the most important and prevalent mycotoxins in animal feeds, health and economic mycotoxins impacts on animals. In addition, the main conventional and advanced approaches in mycotoxins analytical detection techniques and decontamination strategies to mitigate and counteract mycotoxin contamination of feedstuffs were also reported. There are different analytical techniques to precisely qualities and quantities mycotoxins. They included Fluorometer, chromatography-based devices and immunological based techniques besides other recent advanced techniques. Various my-cotoxins detoxification strategies have been developed included physical, chemical and biological strategies to reduce or eliminate mycotoxins in feed ingredients or complete compound feeds, however they cannot totally de-contaminate mycotoxins. Hence, they varied in their limitations or abilities to meet the requirements of practical application according to many factors including their binding efficiency, environmental protection, feeds and foods safety, palatability or cost-effectiveness.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"7 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140354094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.21608/ejah.2024.347395
Mayada A.M. Abou Zeid, A. Salim, Haneen A. Ghazy
A n investigation into the incidence of E. coli bacterial infections linked to outbreaks of rabbit diarrhea was carried out using a field survey. Ninety samples were recovered from diseased and freshly dead rabbits, suffering from diarrhea , from Kafrelsheikh governorate farms. Samples of fecal swabs and Internal organs, including the liver, spleen, and intestinal contents, were aseptically collected, and E. coli was isolated and identified by traditional method . E.coli was typed serologically and tested for antimicrobial agents. E.coli infection incidence rate was (66.6%). More-over, the serologically identified of seven E. coli isolates were three O91, two O128 , one O17 and one O44 .All bacterial isolates were highly sensitive to Ciprofloxacin , Amikacin, and Fosfomycin by 100% , while tetracycline and streptomycin resistance was present in 90% and 40% of the strains, respectively. The isolates were screened for presence of cnf1 and tsh virulence genes , qnrA and tetA(A) antibiotic resistance genes . Six weeks-old thirty healthy rabbits were used , fecal swabs were taken to make sure that rabbits free from E.coli . Rabbits were divided into 5 groups (6 rabbits for each) ; Group 1 were kept as negative control (without infection) , Group 2,3,4,5 were inoculated orally with 1 ml of culture (1x10 7 CFU/ml) , Group 2 were kept as positive control (without treatment) and tested for the virulence by experimental examination , Group 3 were treated with ciprofloxacin 2 days after infection for 5 successive days , Group 4 treated with acetic acid 2 days after infection for 5 successive days , while Group 5 were treated with ciprofloxacin and acetic acid 2 days after infection for 5 successive days . All animals were kept for 21 days (period of observation) with daily examination for clinical signs, mortality rate and gross P.M. lesions in dead animals till the end of the observation period and trials of reisolation were conducted .
{"title":"Role of acetic acid as antibacterial on E.coli isolated from young rabbits","authors":"Mayada A.M. Abou Zeid, A. Salim, Haneen A. Ghazy","doi":"10.21608/ejah.2024.347395","DOIUrl":"https://doi.org/10.21608/ejah.2024.347395","url":null,"abstract":"A n investigation into the incidence of E. coli bacterial infections linked to outbreaks of rabbit diarrhea was carried out using a field survey. Ninety samples were recovered from diseased and freshly dead rabbits, suffering from diarrhea , from Kafrelsheikh governorate farms. Samples of fecal swabs and Internal organs, including the liver, spleen, and intestinal contents, were aseptically collected, and E. coli was isolated and identified by traditional method . E.coli was typed serologically and tested for antimicrobial agents. E.coli infection incidence rate was (66.6%). More-over, the serologically identified of seven E. coli isolates were three O91, two O128 , one O17 and one O44 .All bacterial isolates were highly sensitive to Ciprofloxacin , Amikacin, and Fosfomycin by 100% , while tetracycline and streptomycin resistance was present in 90% and 40% of the strains, respectively. The isolates were screened for presence of cnf1 and tsh virulence genes , qnrA and tetA(A) antibiotic resistance genes . Six weeks-old thirty healthy rabbits were used , fecal swabs were taken to make sure that rabbits free from E.coli . Rabbits were divided into 5 groups (6 rabbits for each) ; Group 1 were kept as negative control (without infection) , Group 2,3,4,5 were inoculated orally with 1 ml of culture (1x10 7 CFU/ml) , Group 2 were kept as positive control (without treatment) and tested for the virulence by experimental examination , Group 3 were treated with ciprofloxacin 2 days after infection for 5 successive days , Group 4 treated with acetic acid 2 days after infection for 5 successive days , while Group 5 were treated with ciprofloxacin and acetic acid 2 days after infection for 5 successive days . All animals were kept for 21 days (period of observation) with daily examination for clinical signs, mortality rate and gross P.M. lesions in dead animals till the end of the observation period and trials of reisolation were conducted .","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"25 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140763497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.21608/ejah.2024.348113
A. M. Hala, Abdelhady, A. Nahla, Ebied, Eman T. Al-Sokary, S. Mostafa, Abdou
: T he objective of study was to determine the rate of isolation of Staphylococcus aureus ( S. aureus ) in normal and mastitic bovine milk and their relationship with the milk composition also microbial, and chemical parameters changes in milk of normal apparently healthy bovine. Moreover, we study the effects of copper oxide Nano particles (Cuo-Nps) and chitosan Nano particles (Ch-Nps) on isolated antibiotic-resistant strains of S. aureus . One hundred milk samples (50 samples from apparently healthy lactating cows and buffaloes 25 for each) and (50 samples of mastitic milk 25 cows’ milk and 25 buffaloes’ milk from the neighboring animals in the same farms) were randomly collected from 10 privet farms (5 cows farm and 5 buffalo's farms) in Kafrelsheikh Governorate, Egypt. Ten of 50 raw milk samples were positive for S.aureus (six raw cow , s milk and 4 raw buffalo , s milk samples) whereas 26 of 50 mastitic milk samples were positive for S.aureus (fifteen mastitic cow , s milk samples and 11 mastitic buffalo , s milk samples). Our results revealed that direct relationship between isolation of S. aureus from normal milk samples and TBC, SCC, minerals (ash)g%, SNF
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