The effect of irisin on the ultrastructure of the thoracic aorta in rat: A morphometric study

Q3 Medicine Morphologie Pub Date : 2024-04-11 DOI:10.1016/j.morpho.2024.100779

A.M. Shurrab , A.T. Shatarat , M.H. Al-Muhtaseb , D.H. Badran , M.A. Salameh , H.M. Al-lahham , R.K. Altaweel , I. Altarawneh , D. Al-Qattan

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Abstract

Background

One of the most recent hormones to be identified and isolated is irisin, extracted from mouse skeletal muscle in 2012. Irisin has been proven to alter blood pressure, which has an impact on blood vessels, enhance endothelial functions, and prevent injury to endothelial cells. The current study aimed to study the effect of irisin on the ultrastructure of the rat thoracic aorta using the transmission electron microscope (TEM).

Materials and methods

Twenty female rats were recruited for this study and divided into a control group (non-injected), and four experimental groups (injected groups) each consisting of 4 rats. The experimental groups were injected intraperitoneally with different doses of irisin (250 ng/mL, 500 ng/mL, 1000 ng/mL, and 2000 ng/mL) twice a week for 4 weeks. Then, the descending thoracic aorta of all experimental rats were resected and proceeded with imaging.

Results

The results of this study showed a change in the thickness of the tunica intima, internal elastic lamina, elastic lamellae, and external elastic lamina concerning increasing injected irisin concentration. While there was a significant increase in the thickness of tunica media (P < 0.0001) and smooth muscle cells (P < 0.05). Also, the results showed a significant increase in the number of elastic lamellae in the tunica media (P < 0.0001).

Conclusion

Irisin had a major impact on the elasticity of the rat thoracic aorta wall, suggesting that it influences the growth factors of the wall and activates smooth muscle cells in addition to endothelial cells.

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鸢尾素对大鼠胸主动脉超微结构的影响：形态计量学研究

背景2012年从小鼠骨骼肌中提取的鸢尾素是最新发现和分离的激素之一。事实证明，鸢尾素能改变血压，从而影响血管，增强内皮功能，防止内皮细胞损伤。本研究旨在利用透射电子显微镜（TEM）研究鸢尾素对大鼠胸主动脉超微结构的影响。材料和方法本研究招募了 20 只雌性大鼠，分为对照组（未注射组）和四个实验组（注射组），每组 4 只。实验组腹腔注射不同剂量的鸢尾素（250 ng/mL、500 ng/mL、1000 ng/mL 和 2000 ng/mL），每周两次，连续注射 4 周。结果本研究结果表明，随着鸢尾素注射浓度的增加，大鼠胸主动脉内膜、内弹力层、弹力层和外弹力层的厚度都发生了变化。而中膜厚度（P< 0.0001）和平滑肌细胞厚度（P< 0.05）有明显增加。结论鸢尾素对大鼠胸主动脉壁的弹性有重大影响，表明它影响了胸主动脉壁的生长因子，并激活了内皮细胞以外的平滑肌细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Morphologie Medicine-Anatomy

CiteScore

2.30

自引率

0.00%

发文量

150

审稿时长

25 days

期刊介绍： Morphologie est une revue universitaire avec une ouverture médicale qui sa adresse aux enseignants, aux étudiants, aux chercheurs et aux cliniciens en anatomie et en morphologie. Vous y trouverez les développements les plus actuels de votre spécialité, en France comme a international. Le objectif de Morphologie est d?offrir des lectures privilégiées sous forme de revues générales, d?articles originaux, de mises au point didactiques et de revues de la littérature, qui permettront notamment aux enseignants de optimiser leurs cours et aux spécialistes d?enrichir leurs connaissances.