Pub Date : 2026-02-02DOI: 10.1016/j.morpho.2025.101103
B O Ibitoye, F F Oloye
Monosodium glutamate (MSG) is a flavour enhancer often added to restaurant foods, canned vegetables, soups, deli meats and other foods. The toxic effects of monosodium glutamate on the male reproductive system have been recognized. This study aimed to investigate the impact of in utero exposure to a high-dose of MSG on the testicular morphometry and physical findings of Wistar rat offspring exposed intra-uterine. Forty (40) adult pregnant Wistar rats (150-200g) were randomly selected and divided into four groups. Group 1 (control) received distilled water, while groups 2, 3 and 4 received 1g/kg, 2g/kg, 4g/kg as 1mL body weight of MSG orally throughout pregnancy respectively. The pups were examined at delivery and allowed to grow for 4 weeks. The animals were sacrificed on the 28th day after delivery. The testes were harvested and weighed. Histomorphometric analysis of the testis were carried out and the morphometric parameters: diameter of seminiferous tubules, cross-sectional area (A) of seminiferous tubules, germinal epithelium thickness, the numerical density, seminiferous tubule numerical density and seminiferous volume were measured using photomicrograph and image J software. Data were analysed using ANOVA at α 0.05. The MSG-treated groups compared to the control group had higher body weights and alteration in the morphology of the testicular tissue. There was also a decrease in the diameter of the seminiferous tubule of the testis with an increasing dose of MSG, a dose-dependent decrease in the cross-sectional area of seminiferous tubules and the thickness of the testis germinal epithelia of the testis. Maternal exposure to high doses of monosodium glutamate adversely affected testicular morphometry and exacerbated testicular tissue alterations.
{"title":"Impact of in utero exposure to high-dose monosodium glutamate on testicular structure and somatic features in Wistar rat progeny.","authors":"B O Ibitoye, F F Oloye","doi":"10.1016/j.morpho.2025.101103","DOIUrl":"https://doi.org/10.1016/j.morpho.2025.101103","url":null,"abstract":"<p><p>Monosodium glutamate (MSG) is a flavour enhancer often added to restaurant foods, canned vegetables, soups, deli meats and other foods. The toxic effects of monosodium glutamate on the male reproductive system have been recognized. This study aimed to investigate the impact of in utero exposure to a high-dose of MSG on the testicular morphometry and physical findings of Wistar rat offspring exposed intra-uterine. Forty (40) adult pregnant Wistar rats (150-200g) were randomly selected and divided into four groups. Group 1 (control) received distilled water, while groups 2, 3 and 4 received 1g/kg, 2g/kg, 4g/kg as 1mL body weight of MSG orally throughout pregnancy respectively. The pups were examined at delivery and allowed to grow for 4 weeks. The animals were sacrificed on the 28th day after delivery. The testes were harvested and weighed. Histomorphometric analysis of the testis were carried out and the morphometric parameters: diameter of seminiferous tubules, cross-sectional area (A) of seminiferous tubules, germinal epithelium thickness, the numerical density, seminiferous tubule numerical density and seminiferous volume were measured using photomicrograph and image J software. Data were analysed using ANOVA at α 0.05. The MSG-treated groups compared to the control group had higher body weights and alteration in the morphology of the testicular tissue. There was also a decrease in the diameter of the seminiferous tubule of the testis with an increasing dose of MSG, a dose-dependent decrease in the cross-sectional area of seminiferous tubules and the thickness of the testis germinal epithelia of the testis. Maternal exposure to high doses of monosodium glutamate adversely affected testicular morphometry and exacerbated testicular tissue alterations.</p>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"110 369","pages":"101103"},"PeriodicalIF":0.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146114277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-27DOI: 10.1016/j.morpho.2025.101104
Y.R. Djembi , R. Abiome , A. Mikiéla , C. Mba Mba , R. Koussou , N. Ndjota , L.S. Bayonne Manou , N. Boumas , F. Ondo Ndong
<div><h3>Introduction</h3><div>As a foundational discipline of medicine, human anatomy has historically been built around dissection. In many cultural contexts, particularly in sub-Saharan Africa, representations of the body and death may limit post-mortem body donation and restrict access to dissection for educational purposes.</div></div><div><h3>Objectives</h3><div>To analyze perceptions of body donation after death and human dissection in a Bantu context, to assess their stability over time, to situate them within an international comparative perspective, and to explore appropriate pedagogical alternatives.</div></div><div><h3>Methods</h3><div>Two successive cross-sectional surveys were conducted in Gabon among participants from the Université des Sciences de la Santé (USS, Libreville) and three regional hospitals: the first in 2013 (<em>n</em> <!-->=<!--> <!-->187) and the second between 2020 and 2025 (<em>n</em> <!-->=<!--> <!-->218). The same questionnaire, comprising 16 items grouped into five domains (motivations, perceptions of medical systems, representations of the body, attitudes toward body donation, and pedagogical resources), was used. A descriptive comparison of anatomy teaching modalities was performed with Cheikh Anta Diop University of Dakar (Senegal) and the University of Burgundy – Europe in Dijon (France).</div></div><div><h3>Results</h3><div>In both surveys, donation of one's own body after death was overwhelmingly rejected (more than 95% of participants), while a majority acknowledged the educational value of human dissection. In the absence of available bodies, the most frequently cited alternative resources were medical imaging, digital tools, and observation of surgical procedures. International comparison revealed marked differences: supervised and systematic dissection in France, occasional practices in Senegal, and the absence of dissection in Gabon.</div></div><div><h3>Discussion</h3><div>The study highlights the stability of representations related to body donation in Gabon, characterized by a persistent rejection of post-mortem donation despite recognition of the pedagogical value of dissection. This dissociation reflects a coexistence of cultural and biomedical rationalities observed in various international contexts. Organizational differences between Gabon, Senegal, and France underscore the need to adapt teaching practices to local constraints by integrating complementary alternatives to dissection.</div></div><div><h3>Perspectives</h3><div>In contexts where access to human dissection remains limited, the progressive development of alternative pedagogical solutions combining digital tools, medical imaging, and anatomical simulation appears to be a pragmatic approach to strengthening anatomy education. In the longer term, the structuring of teaching and simulation facilities adapted to local cultural and institutional specificities could contribute to sustainable improvement in medical training.</div></div><di
{"title":"Teaching anatomy in the Bantu context: Traditions, cultural issues and educational perspectives in Gabon","authors":"Y.R. Djembi , R. Abiome , A. Mikiéla , C. Mba Mba , R. Koussou , N. Ndjota , L.S. Bayonne Manou , N. Boumas , F. Ondo Ndong","doi":"10.1016/j.morpho.2025.101104","DOIUrl":"10.1016/j.morpho.2025.101104","url":null,"abstract":"<div><h3>Introduction</h3><div>As a foundational discipline of medicine, human anatomy has historically been built around dissection. In many cultural contexts, particularly in sub-Saharan Africa, representations of the body and death may limit post-mortem body donation and restrict access to dissection for educational purposes.</div></div><div><h3>Objectives</h3><div>To analyze perceptions of body donation after death and human dissection in a Bantu context, to assess their stability over time, to situate them within an international comparative perspective, and to explore appropriate pedagogical alternatives.</div></div><div><h3>Methods</h3><div>Two successive cross-sectional surveys were conducted in Gabon among participants from the Université des Sciences de la Santé (USS, Libreville) and three regional hospitals: the first in 2013 (<em>n</em> <!-->=<!--> <!-->187) and the second between 2020 and 2025 (<em>n</em> <!-->=<!--> <!-->218). The same questionnaire, comprising 16 items grouped into five domains (motivations, perceptions of medical systems, representations of the body, attitudes toward body donation, and pedagogical resources), was used. A descriptive comparison of anatomy teaching modalities was performed with Cheikh Anta Diop University of Dakar (Senegal) and the University of Burgundy – Europe in Dijon (France).</div></div><div><h3>Results</h3><div>In both surveys, donation of one's own body after death was overwhelmingly rejected (more than 95% of participants), while a majority acknowledged the educational value of human dissection. In the absence of available bodies, the most frequently cited alternative resources were medical imaging, digital tools, and observation of surgical procedures. International comparison revealed marked differences: supervised and systematic dissection in France, occasional practices in Senegal, and the absence of dissection in Gabon.</div></div><div><h3>Discussion</h3><div>The study highlights the stability of representations related to body donation in Gabon, characterized by a persistent rejection of post-mortem donation despite recognition of the pedagogical value of dissection. This dissociation reflects a coexistence of cultural and biomedical rationalities observed in various international contexts. Organizational differences between Gabon, Senegal, and France underscore the need to adapt teaching practices to local constraints by integrating complementary alternatives to dissection.</div></div><div><h3>Perspectives</h3><div>In contexts where access to human dissection remains limited, the progressive development of alternative pedagogical solutions combining digital tools, medical imaging, and anatomical simulation appears to be a pragmatic approach to strengthening anatomy education. In the longer term, the structuring of teaching and simulation facilities adapted to local cultural and institutional specificities could contribute to sustainable improvement in medical training.</div></div><di","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"110 369","pages":"Article 101104"},"PeriodicalIF":0.0,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-27DOI: 10.1016/j.morpho.2025.101105
Marco P. Barros Pinto
A preliminary diagnosis of thrombocytopenia was made in a 79-year-old woman based on the results of a complete blood count. During the analysis of a peripheral blood film, platelet satellitism was noted. Platelet satellitism can result in pseudothrombocytopenia or a spurious neutrophil count in automated analyzers.
{"title":"Platelet satellitism as a cause of pseudothrombocytopenia","authors":"Marco P. Barros Pinto","doi":"10.1016/j.morpho.2025.101105","DOIUrl":"10.1016/j.morpho.2025.101105","url":null,"abstract":"<div><div>A preliminary diagnosis of thrombocytopenia was made in a 79-year-old woman based on the results of a complete blood count. During the analysis of a peripheral blood film, platelet satellitism was noted. Platelet satellitism can result in pseudothrombocytopenia or a spurious neutrophil count in automated analyzers.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"110 369","pages":"Article 101105"},"PeriodicalIF":0.0,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-13DOI: 10.1016/j.morpho.2025.101102
P. Ndahimana , S. Habumuremyi , C. Niyibigira , V.B. Archibong , A. Okesina , T. Twagirumugabe , J. Gashegu
Background
Anatomical variation in the brachial plexus exists at different levels. Knowledge on the variations in terminal branches is of utmost importance especially to anesthesiologists and surgeons for peripheral nerve blocks’ approaches and avoidance of perioperative complications during surgery respectively. We conducted this study to evaluate major variations in the local context.
Objective
The study was done to determine the anatomical variations in terminal branches of the brachial plexus among cadavers recruited from hospitals across Rwanda.
Materials and methods
Forty-eight brachial plexus (24 human donors) were dissected and examined to assess the branching pattern and possible variations of terminal branches. All specimens were formalin-fixed and dissected. Nerves were identified macroscopically with nacked eyes. To be more specific in dissection, magnifiers were used for some nerves in order to visualize them with much of precision. The study was approved by Institutional Review Board of the University of Rwanda.
Results
Forty-eight including 24 left and 24 right brachial plexuses (24 human donors) were dissected. All human bodies were adult aged between 28 and 72 years old. Considering gender disparity, 23/24 (96%) were male, only 1/24 (4%) was a female. From the 48 brachial plexus, 14 (29.2%) of them showed variations of its terminal branches where 9 cases (18.8%) are related to the variation of median nerve and 5 (10.4%) are related to other terminal branches. These deviations from the classical formation of the terminal branches may pose risks during surgical or anesthetic procedures if not known. They may also pose diagnostical challenges.
Conclusion
Anatomical variation of terminal branches of the brachial plexus are important and should be taken into consideration during clinical procedures for effective diagnosis and management of patients.
{"title":"Variations in the terminal branches of the brachial plexus in humans. A cadaveric study","authors":"P. Ndahimana , S. Habumuremyi , C. Niyibigira , V.B. Archibong , A. Okesina , T. Twagirumugabe , J. Gashegu","doi":"10.1016/j.morpho.2025.101102","DOIUrl":"10.1016/j.morpho.2025.101102","url":null,"abstract":"<div><h3>Background</h3><div>Anatomical variation in the brachial plexus exists at different levels. Knowledge on the variations in terminal branches is of utmost importance especially to anesthesiologists and surgeons for peripheral nerve blocks’ approaches and avoidance of perioperative complications during surgery respectively. We conducted this study to evaluate major variations in the local context.</div></div><div><h3>Objective</h3><div>The study was done to determine the anatomical variations in terminal branches of the brachial plexus among cadavers recruited from hospitals across Rwanda.</div></div><div><h3>Materials and methods</h3><div>Forty-eight brachial plexus (24 human donors) were dissected and examined to assess the branching pattern and possible variations of terminal branches. All specimens were formalin-fixed and dissected. Nerves were identified macroscopically with nacked eyes. To be more specific in dissection, magnifiers were used for some nerves in order to visualize them with much of precision. The study was approved by Institutional Review Board of the University of Rwanda.</div></div><div><h3>Results</h3><div>Forty-eight including 24 left and 24 right brachial plexuses (24 human donors) were dissected. All human bodies were adult aged between 28 and 72 years old. Considering gender disparity, 23/24 (96%) were male, only 1/24 (4%) was a female. From the 48 brachial plexus, 14 (29.2%) of them showed variations of its terminal branches where 9 cases (18.8%) are related to the variation of median nerve and 5 (10.4%) are related to other terminal branches. These deviations from the classical formation of the terminal branches may pose risks during surgical or anesthetic procedures if not known. They may also pose diagnostical challenges.</div></div><div><h3>Conclusion</h3><div>Anatomical variation of terminal branches of the brachial plexus are important and should be taken into consideration during clinical procedures for effective diagnosis and management of patients.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"110 368","pages":"Article 101102"},"PeriodicalIF":0.0,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-12DOI: 10.1016/j.morpho.2025.101100
Rawad Qaq , Ademir Franco , Scheila Manica
Dental morphological traits provide valuable insights into global human variation and population structure. The Arizona State University Dental Anthropology System (ASUDAS) has long been a cornerstone for documenting crown and root morphology, yet recent discussions have questioned its reliability for characterising population differences. This study aimed to (i) identify the ASUDAS traits most commonly analysed in recent research, and (ii) compare their reported frequencies with those documented in the foundational work of Turner and Scott. A systematic review identified 43 eligible studies comprising 36,919 individuals representing all major subdivisions of humankind. Thirty variants across 27 distinct traits were recorded most frequently, and 29 of these were subjected to meta-analysis using random-effects models. Nearly all traits showed significant between-group and within-group heterogeneity, indicating strong discriminatory potential but also substantial population-level variability. While broad patterns remain consistent with classic ASUDAS expectations, several traits exhibited expanded regional ranges, reflecting both greater sample diversity and admixture effects. These findings reaffirm the value of ASUDAS traits for describing broad-scale morphological patterns while underscoring the need for multi-trait, population-specific, and context-specific approaches when interpreting dental morphology in anthropological research.
{"title":"Global patterns of dental morphological variation: Revisiting ASUDAS trait frequencies","authors":"Rawad Qaq , Ademir Franco , Scheila Manica","doi":"10.1016/j.morpho.2025.101100","DOIUrl":"10.1016/j.morpho.2025.101100","url":null,"abstract":"<div><div>Dental morphological traits provide valuable insights into global human variation and population structure. The Arizona State University Dental Anthropology System (ASUDAS) has long been a cornerstone for documenting crown and root morphology, yet recent discussions have questioned its reliability for characterising population differences. This study aimed to (i) identify the ASUDAS traits most commonly analysed in recent research, and (ii) compare their reported frequencies with those documented in the foundational work of Turner and Scott. A systematic review identified 43 eligible studies comprising 36,919 individuals representing all major subdivisions of humankind. Thirty variants across 27 distinct traits were recorded most frequently, and 29 of these were subjected to meta-analysis using random-effects models. Nearly all traits showed significant between-group and within-group heterogeneity, indicating strong discriminatory potential but also substantial population-level variability. While broad patterns remain consistent with classic ASUDAS expectations, several traits exhibited expanded regional ranges, reflecting both greater sample diversity and admixture effects. These findings reaffirm the value of ASUDAS traits for describing broad-scale morphological patterns while underscoring the need for multi-trait, population-specific, and context-specific approaches when interpreting dental morphology in anthropological research.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"110 368","pages":"Article 101100"},"PeriodicalIF":0.0,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sex estimation plays a critical role in forensic identification, missing person identification, and forensic investigations. This study aimed to evaluate the usability of quantitative metric measurements obtained from computed tomography (CT) images of the atlas (the first cervical vertebra) for sex identification using machine learning algorithms. The study used CT images from 200 individuals (100 males and 100 females). Eighteen metric parameters of the atlas—comprising 16 lengths and 2 angles—were measured. These parameters were analyzed using 13 different machine learning algorithms. Basic statistical methods were used to compare the effect of each metric parameter on sex estimation. The machine learning models predicted sex with an accuracy ranging from 86% to 89%. The highest accuracy (89%) was achieved by the Gaussian Naive Bayes algorithm using only five selected metric parameters. Additionally, 15 out of the 18 measured parameters showed statistically significant differences between sexes. This study demonstrates that sex can be estimated with high accuracy using only quantitative metric measurements data from the atlas vertebra and machine learning algorithms. Notably, this approach may be especially valuable when only the atlas is available, providing essential preliminary data for forensic medical examinations.
{"title":"Usability of quantitative atlas measurements from computed tomography images for sex estimation: A machine learning approach","authors":"Mensure Sahin , Muhammed Kamil Turan , Zulal Oner , Serkan Oner","doi":"10.1016/j.morpho.2025.101101","DOIUrl":"10.1016/j.morpho.2025.101101","url":null,"abstract":"<div><div>Sex estimation plays a critical role in forensic identification, missing person identification, and forensic investigations. This study aimed to evaluate the usability of quantitative metric measurements obtained from computed tomography (CT) images of the atlas (the first cervical vertebra) for sex identification using machine learning algorithms. The study used CT images from 200 individuals (100 males and 100 females). Eighteen metric parameters of the atlas—comprising 16 lengths and 2 angles—were measured. These parameters were analyzed using 13 different machine learning algorithms. Basic statistical methods were used to compare the effect of each metric parameter on sex estimation. The machine learning models predicted sex with an accuracy ranging from 86% to 89%. The highest accuracy (89%) was achieved by the Gaussian Naive Bayes algorithm using only five selected metric parameters. Additionally, 15 out of the 18 measured parameters showed statistically significant differences between sexes. This study demonstrates that sex can be estimated with high accuracy using only quantitative metric measurements data from the atlas vertebra and machine learning algorithms. Notably, this approach may be especially valuable when only the atlas is available, providing essential preliminary data for forensic medical examinations.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"110 368","pages":"Article 101101"},"PeriodicalIF":0.0,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-05DOI: 10.1016/j.morpho.2025.101099
A.F. de Oliveira , B.P. Zanovello , D.D. Moreira , A. Franco
Dental age assessment may have important clinical and forensic applications. Testing the existing methods is fundamental to promoting best clinical and forensic practices. The present study aimed to test a Brazilian country-specific model for dental age assessment in a population of Bahia State, Brazil. A total of 1009 panoramic radiographs of Brazilian individuals (n = 556, 55.1% females and 453, 44.9% males) aged between 6 and 15.99 years were collected. The seven permanent left mandibular teeth from central incisor to second molar were analyzed in each radiograph. Demirjian's dental staging technique was applied followed by age estimation through Franco's dental age assessment model. Differences between chronological and estimated ages were quantified as mean error (ME), mean absolute error (MAE), and root means squared error (RMSE). The overall (sex combined) ME, MAE, RMSE were −0.51, 1.11, 1.63, respectively. For females and males separately, the error metrics were: −0.55, 1.16, 1.65 and −0.45, 1.05, 1.59, respectively. Bland-Altman plots showed narrower age limits among males, while higher discrepancies were observed for females. The analysis based on age categories showed the worst predictions between the ages of 6 and 6.99 years. The tested country-specific model was applicable to individuals aged above 7 years in the target population, being an alternative for dental age assessment.
{"title":"External validation of a country-specific model for radiographic dental age assessment of Brazilian children and adolescents","authors":"A.F. de Oliveira , B.P. Zanovello , D.D. Moreira , A. Franco","doi":"10.1016/j.morpho.2025.101099","DOIUrl":"10.1016/j.morpho.2025.101099","url":null,"abstract":"<div><div>Dental age assessment may have important clinical and forensic applications. Testing the existing methods is fundamental to promoting best clinical and forensic practices. The present study aimed to test a Brazilian country-specific model for dental age assessment in a population of Bahia State, Brazil. A total of 1009 panoramic radiographs of Brazilian individuals (<em>n</em> <!-->=<!--> <!-->556, 55.1% females and 453, 44.9% males) aged between 6 and 15.99 years were collected. The seven permanent left mandibular teeth from central incisor to second molar were analyzed in each radiograph. Demirjian's dental staging technique was applied followed by age estimation through Franco's dental age assessment model. Differences between chronological and estimated ages were quantified as mean error (ME), mean absolute error (MAE), and root means squared error (RMSE). The overall (sex combined) ME, MAE, RMSE were −0.51, 1.11, 1.63, respectively. For females and males separately, the error metrics were: −0.55, 1.16, 1.65 and −0.45, 1.05, 1.59, respectively. Bland-Altman plots showed narrower age limits among males, while higher discrepancies were observed for females. The analysis based on age categories showed the worst predictions between the ages of 6 and 6.99 years. The tested country-specific model was applicable to individuals aged above 7 years in the target population, being an alternative for dental age assessment.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"110 368","pages":"Article 101099"},"PeriodicalIF":0.0,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145684432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-26DOI: 10.1016/j.morpho.2025.100990
Abdulrahman Alblowi , Nicla Settembre , Manuela Perez , Marc Braun , Guillaume Gauchotte , Sergueï Malikov
Introduction
L’utilisation de matériel autologue pour remplacer un vaisseau dans un champ infecté est recommandée en chirurgie vasculaire [1], [2]. Le patch aponévrotique postérieur du péritoine pariétal (PAPP) se compose d’une couche interne péritonéale et d’une couche aponeurotique renforcée par les aponevroses des muscles abdominales [3], [4]. L’objectif de cette étude est d’évaluer la faisabilité technique de la reconstruction d’un tube de PAPP pour l’aorte abdominale, en explorant ses caractéristiques histologiques, en vue de son utilisation en absence d’autres vaisseaux autologues disponibles.
Méthodes
Six dissections sur cadavres frais ont été réalisées pour prélever le tissu de PAPP bilatéralement, après avoir séparé le muscle du fascia. Des mesures de PAPP prélevable ont été prises, puis un tube PAPP a été construit. Ensuite, trois échantillons ont été prélevés sur chaque patch pour une analyse histologique avec coloration Hématoxyline-Éosine-Safran (HES) et Weigert.
Résultats
Les dissections anatomiques ont montré une longueur totale moyenne de 12,6 cm, avec une zone triangulaire supérieure de 3,17 cm. La largeur de PAPP 2 était de 7,67 cm, suffisante pour reconstruire un tube avec un diamètre minimum de 23,55 mm. L’analyse histologique a révélé une présence minimale de tissu adipeux entre les couches péritonéale et aponeurotique, avec une structure en deux couches distinctes : péritonéale (mésothéliale et sous-mésothéliale) et aponeurotique, composée d’une couche élastique interne, d’une couche de fibres de collagène, d’une couche élastique externe et d’un revêtement externe de tissu fibreux.
Conclusion
Notre étude confirme la faisabilité technique de reconstruire un tube de PAPP pour l’aorte abdominale sous-rénale. Les résultats anatomiques montrent des dimensions adaptées pour les applications cliniques au niveau de l’aorte abdominale. L’analyse histologique révèle deux couches distinctes, péritonéale et aponeurotique, aux caractéristiques robustes. La similitude relative entre l’intima et le péritoine, ainsi qu’entre le média et le plan aponévrotique, rend le PAPP un matériel prometteur pour la reconstruction aortique en absence d’autres tissus autologues.
{"title":"Étude anatomique et histologique de la faisabilité d’utilisation du tissu aponévrotique postérieur du péritoine pariétal comme substitut vasculaire","authors":"Abdulrahman Alblowi , Nicla Settembre , Manuela Perez , Marc Braun , Guillaume Gauchotte , Sergueï Malikov","doi":"10.1016/j.morpho.2025.100990","DOIUrl":"10.1016/j.morpho.2025.100990","url":null,"abstract":"<div><h3>Introduction</h3><div>L’utilisation de matériel autologue pour remplacer un vaisseau dans un champ infecté est recommandée en chirurgie vasculaire <span><span>[1]</span></span>, <span><span>[2]</span></span>. Le patch aponévrotique postérieur du péritoine pariétal (PAPP) se compose d’une couche interne péritonéale et d’une couche aponeurotique renforcée par les aponevroses des muscles abdominales <span><span>[3]</span></span>, <span><span>[4]</span></span>. L’objectif de cette étude est d’évaluer la faisabilité technique de la reconstruction d’un tube de PAPP pour l’aorte abdominale, en explorant ses caractéristiques histologiques, en vue de son utilisation en absence d’autres vaisseaux autologues disponibles.</div></div><div><h3>Méthodes</h3><div>Six dissections sur cadavres frais ont été réalisées pour prélever le tissu de PAPP bilatéralement, après avoir séparé le muscle du fascia. Des mesures de PAPP prélevable ont été prises, puis un tube PAPP a été construit. Ensuite, trois échantillons ont été prélevés sur chaque patch pour une analyse histologique avec coloration Hématoxyline-Éosine-Safran (HES) et Weigert.</div></div><div><h3>Résultats</h3><div>Les dissections anatomiques ont montré une longueur totale moyenne de 12,6<!--> <!-->cm, avec une zone triangulaire supérieure de 3,17<!--> <!-->cm. La largeur de PAPP 2 était de 7,67<!--> <!-->cm, suffisante pour reconstruire un tube avec un diamètre minimum de 23,55<!--> <!-->mm. L’analyse histologique a révélé une présence minimale de tissu adipeux entre les couches péritonéale et aponeurotique, avec une structure en deux couches distinctes : péritonéale (mésothéliale et sous-mésothéliale) et aponeurotique, composée d’une couche élastique interne, d’une couche de fibres de collagène, d’une couche élastique externe et d’un revêtement externe de tissu fibreux.</div></div><div><h3>Conclusion</h3><div>Notre étude confirme la faisabilité technique de reconstruire un tube de PAPP pour l’aorte abdominale sous-rénale. Les résultats anatomiques montrent des dimensions adaptées pour les applications cliniques au niveau de l’aorte abdominale. L’analyse histologique révèle deux couches distinctes, péritonéale et aponeurotique, aux caractéristiques robustes. La similitude relative entre l’intima et le péritoine, ainsi qu’entre le média et le plan aponévrotique, rend le PAPP un matériel prometteur pour la reconstruction aortique en absence d’autres tissus autologues.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"109 367","pages":"Article 100990"},"PeriodicalIF":0.0,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145600373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-26DOI: 10.1016/j.morpho.2025.101000
Alexandre Bellier , Ariane Weyl , Volodia Barriere-Voldnarek , Peter D. Lee , Claire Walsh , Paul Tafforeau
Objet
Apparu dans la majorité des livres d’anatomie il y a quelques dizaines d’années seulement, le clitoris reste peu représenté [1]. La micro-anatomie du clitoris est ainsi peu étudiée, comme le montre la persistance du débat sur l’origine et la composition du point G [2]. L’imagerie synchrotron apparaît comme une technique prometteuse pour l’exploration à haute résolution des tissus mous [3]. L’objectif de cette étude était l’analyse micro-anatomique d’un pelvis féminin par imagerie synchrotron hiérarchique à contraste de phase.
Méthodes
Un prélèvement en bloc de l’ensemble des organes pelviens a été réalisé sur un corps anatomique féminin de 91 ans (55 kg pour 1,55 m) au Laboratoire d’anatomie des Alpes françaises. La pièce anatomique a été préparée et imagé au Synchrotron européen à 16,748 μm de résolution avec des zooms à 2 μm, selon la technique de tomographie hiérarchique par rayonnement synchrotron à contraste de phase [3]. Une segmentation semi-automatique du clitoris et des organes adjacents a été réalisée.
Résultats
L’imagerie a permis de visualiser l’ensemble des composants du clitoris de sa partie externe (gland) de 5,6 mm de long, au corps médian de 12,5 mm jusqu’aux structures paires et symétriques caverneuses (39,4 mm) ou spongieuses (31,5 mm). L’imagerie a permis la visualisation des structures vasculaires et nerveuses associées au clitoris. Par ailleurs, le point G a été retrouvé dans la paroi antérieure et distale du vagin. Reconnaissable à son aspect glandulaire, cette zone de 7 mm de diamètre située à proximité de l’urètre est séparée des corps clitoridiens. Nous proposons la première représentation tridimensionnelle du clitoris et du point G à haute résolution permettant de mieux appréhender l’anatomie de ces structures et leurs rapports.
Conclusion
L’imagerie synchrotron à contraste de phase a permis de manière inédite la visualisation tridimensionnelle à très haute résolution du clitoris et du point G permettant de mieux appréhender cette anatomie complexe et encore méconnue.
{"title":"Au-delà des mythes, lumière synchrotron sur l’anatomie du clitoris et du point G en imagerie hiérarchique à contraste de phase","authors":"Alexandre Bellier , Ariane Weyl , Volodia Barriere-Voldnarek , Peter D. Lee , Claire Walsh , Paul Tafforeau","doi":"10.1016/j.morpho.2025.101000","DOIUrl":"10.1016/j.morpho.2025.101000","url":null,"abstract":"<div><h3>Objet</h3><div>Apparu dans la majorité des livres d’anatomie il y a quelques dizaines d’années seulement, le clitoris reste peu représenté <span><span>[1]</span></span>. La micro-anatomie du clitoris est ainsi peu étudiée, comme le montre la persistance du débat sur l’origine et la composition du point G <span><span>[2]</span></span>. L’imagerie synchrotron apparaît comme une technique prometteuse pour l’exploration à haute résolution des tissus mous <span><span>[3]</span></span>. L’objectif de cette étude était l’analyse micro-anatomique d’un pelvis féminin par imagerie synchrotron hiérarchique à contraste de phase.</div></div><div><h3>Méthodes</h3><div>Un prélèvement en bloc de l’ensemble des organes pelviens a été réalisé sur un corps anatomique féminin de 91 ans (55<!--> <!-->kg pour 1,55<!--> <!-->m) au Laboratoire d’anatomie des Alpes françaises. La pièce anatomique a été préparée et imagé au Synchrotron européen à 16,748<!--> <!-->μm de résolution avec des zooms à 2<!--> <!-->μm, selon la technique de tomographie hiérarchique par rayonnement synchrotron à contraste de phase <span><span>[3]</span></span>. Une segmentation semi-automatique du clitoris et des organes adjacents a été réalisée.</div></div><div><h3>Résultats</h3><div>L’imagerie a permis de visualiser l’ensemble des composants du clitoris de sa partie externe (gland) de 5,6<!--> <!-->mm de long, au corps médian de 12,5<!--> <!-->mm jusqu’aux structures paires et symétriques caverneuses (39,4<!--> <!-->mm) ou spongieuses (31,5<!--> <!-->mm). L’imagerie a permis la visualisation des structures vasculaires et nerveuses associées au clitoris. Par ailleurs, le point G a été retrouvé dans la paroi antérieure et distale du vagin. Reconnaissable à son aspect glandulaire, cette zone de 7<!--> <!-->mm de diamètre située à proximité de l’urètre est séparée des corps clitoridiens. Nous proposons la première représentation tridimensionnelle du clitoris et du point G à haute résolution permettant de mieux appréhender l’anatomie de ces structures et leurs rapports.</div></div><div><h3>Conclusion</h3><div>L’imagerie synchrotron à contraste de phase a permis de manière inédite la visualisation tridimensionnelle à très haute résolution du clitoris et du point G permettant de mieux appréhender cette anatomie complexe et encore méconnue.</div></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":"109 367","pages":"Article 101000"},"PeriodicalIF":0.0,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145600422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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