Pea G-protein γ subunits: Unlocking their potential in physiological stress and mycorrhizal mediated nutrient sensing

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2024-04-16 DOI:10.1016/j.cpb.2024.100344
Deepak Bhardwaj , Jyoti Priya Samantaray , Varshmeen Kour , Jahanvi Ganotra , Rachana Verma , Asha Chaubey , Tanushri Kaul , Suman Lakhanpaul , Narendra Tuteja
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Abstract

Heterotrimeric GTP-binding proteins or G-proteins are pivotal players in the intricate signaling cascades of plant cells, operating through their binding to guanine nucleotides. These G-proteins primarily consist of three essential subunits: Gα, Gβ, and Gγ. Among these subunits, Gγ stands out for its remarkable genetic diversity. In the present investigation, six Gγ subunits were identified in the Indian variety T-163 of the pea plant (Pisum sativum). Notably, two of these novel Gγ subunits, named PsGγ1 and PsGγ2, belong to type A, while PsGγ3 falls within the type B category. The remaining three subunits, namely PsGγ4, PsGγ5, and PsGγ6, are classified under type C. An in-depth comparison of the amino acid sequences of these pea Gγ subunits with their counterparts in other plants, including Arabidopsis thaliana and Oryza sativa, has unveiled significant variations. This research explores the impact of different treatments on PsGγ genes (PsGγ1 to PsGγ6) in plants. Noteworthy discoveries include a 4-fold increase in the expression of PsGγ1, PsGγ4, PsGγ5, and PsGγ6 in the presence of nitrogen. PsGγ2 and PsGγ3, however, show no response. Phosphorus induces a 3-fold upregulation in PsGγ2 and PsGγ4, and a 4-fold increase in PsGγ5. Conversely, the absence of phosphorus triggers a 4-fold upregulation in PsGγ4 and PsGγ5. Heat stress leads to a 3-fold upregulation in PsGγ2, PsGγ4, and PsGγ5, while cold stress results in a 3-fold upregulation of PsGγ1 and PsGγ6. Under high salt conditions, PsGγ1, PsGγ3, PsGγ4, and PsGγ6 exhibit a 4-fold upregulation, with PsGγ2 showing a 2-fold increase. PsGγ4 and PsGγ5 display a 4-fold upregulation in response to ABA, while PsGγ2 and PsGγ3 show a 3-fold increase while MeJA induces a 4-fold upregulation in PsGγ5. Notably, this study unveils, for the first time, the significant role of Gγ subunits during endosymbiotic associations with phosphorus-acquiring AMF with AMF triggering a 4-fold upregulation in PsGγ4 and PsGγ6. The presence of multiple Gγ subunits in pea underscores their critical participation in governing plant development, stress responses, nutrient sensing, and interactions with mycorrhizal fungi.

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豌豆 G 蛋白 γ 亚基:发掘其在生理压力和菌根介导的养分感应中的潜力
异三聚体 GTP 结合蛋白或 G 蛋白是植物细胞复杂信号级联中的关键角色,通过与鸟嘌呤核苷酸结合发挥作用。这些 G 蛋白主要由三个基本亚基组成:Gα、Gβ 和 Gγ。在这些亚基中,Gγ因其显著的遗传多样性而脱颖而出。本研究在印度豌豆(Pisum sativum)品种 T-163 中发现了六个 Gγ 亚基。值得注意的是,其中两个新的 Gγ 亚基,即 PsGγ1 和 PsGγ2 属于 A 型,而 PsGγ3 属于 B 型。对这些豌豆 Gγ 亚基的氨基酸序列与其他植物(包括拟南芥和黑麦草)的氨基酸序列进行深入比较后发现,它们之间存在显著差异。这项研究探讨了不同处理方法对植物中 PsGγ 基因(PsGγ1 至 PsGγ6)的影响。值得注意的发现包括在氮的存在下,PsGγ1、PsGγ4、PsGγ5 和 PsGγ6 的表达量增加了 4 倍。然而,PsGγ2 和 PsGγ3 没有任何反应。磷诱导 PsGγ2 和 PsGγ4 上调 3 倍,PsGγ5 上调 4 倍。相反,缺磷会导致 PsGγ4 和 PsGγ5 上调 4 倍。热胁迫导致 PsGγ2 、PsGγ4 和 PsGγ5 上调 3 倍,而冷胁迫则导致 PsGγ1 和 PsGγ6 上调 3 倍。在高盐条件下,PsGγ1、PsGγ3、PsGγ4 和 PsGγ6 上调 4 倍,PsGγ2 上调 2 倍。PsGγ4 和 PsGγ5 对 ABA 的响应上调了 4 倍,PsGγ2 和 PsGγ3 上调了 3 倍,而 MeJA 则诱导 PsGγ5 上调了 4 倍。值得注意的是,本研究首次揭示了 Gγ 亚基在与磷获取型 AMF 的内共生过程中的重要作用,AMF 会引发 PsGγ4 和 PsGγ6 的上调 4 倍。豌豆中多种 Gγ 亚基的存在突出表明,它们在管理植物发育、胁迫反应、养分感应以及与菌根真菌的相互作用方面发挥着重要作用。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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