Circular RNA ath-circ032768, a competing endogenous RNA, response the drought stress by targeting miR472-RPS5 module

IF 4.2 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2024-04-08 DOI:10.1111/plb.13645
Z. Yin, Q. Zhao, X. Lv, X. Zhang, Y. Wu
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环状 RNA ath-circ032768(一种竞争性内源 RNA)通过靶向 miR472-RPS5 模块响应干旱胁迫
循环 RNA(环状 RNA)通过 ceRNA(竞争性内源 RNA)降低 miRNA 的丰度,从而调节植物的许多生理过程和胁迫反应。然而,人们对 circRNA 在干旱胁迫中的作用知之甚少。通过对ath-circ032768的环鉴定和测序验证,生物信息学分析预测了ath-circ032768-miR472-RPS5的相互作用,并进一步获得了过表达ath-circ032768和沉默STM-miR472的转基因植株。利用生化和分子生物学方法分析了干旱胁迫的变化。测序和生物学分析证实,ath-circ032768、miR472和RPS5对干旱胁迫有响应,基因表达的变化与ceRNA的预测一致。利用分子生物学技术构建了沉默载体 ath-circ032768 和 STTM-miR472,并获得了具有抗旱性的稳定转基因植株。进一步的生理生化研究表明,ath-circ032768能与miR472结合,而miR472能与RPS5基因结合,从而导致RPS5表达量减少。因此,ath-circ032768 可以通过 ceRNA 竞争性地抑制 miR472 对 RPS5 的降解。这一过程伴随着 DREB2A、RD29A 和 RD29B 基因表达的增加。通过 ath-circ032768-miR472-RPS5 通路,RPS5 抗逆蛋白与 DREB2A 蛋白相互作用,增强下游抗旱基因 RD29A 和 RD29B 的表达,参与植物抗旱调控机制,从而提高植物的抗旱性。
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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
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