MicroRNA Regulatory Pattern in Diabetic Mouse Cortex at Different Stages Following Ischemic Stroke

IF 2.7 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Molecular Neuroscience Pub Date : 2024-04-03 DOI:10.1007/s12031-024-02207-5

Yifei Lv, Guanghui Xie, Yujie Xi, Liu Zhang, Jiajun Wang, Jianhua Wu

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Abstract

After ischemic stroke, microRNAs (miRNAs) participate in various processes, including immune responses, inflammation, and angiogenesis. Diabetes is a key factor increasing the risk of ischemic stroke; however, the regulatory pattern of miRNAs at different stages of diabetic stroke remains unclear. This study comprehensively analyzed the miRNA expression profiles in diabetic mice at 1, 3, and 7 days post-reperfusion following the middle cerebral artery occlusion (MCAO). We identified differentially expressed (DE) miRNAs in diabetic stroke and found significant dysregulation of some novel miRNAs (novel_mir310, novel_mir89, and novel_mir396) post-stroke. These DEmiRNAs were involved in apoptosis and the formation of tight junctions. Finally, we identified three groups of time-dependent DE miRNAs (miR-6240, miR-135b-3p, and miR-672-5p). These have the potential to serve as biomarkers of diabetic stroke. These findings provide a new perspective for future research, emphasizing the dynamic changes in miRNA expression after diabetic stroke and offering potential candidates as biomarkers for future clinical applications.

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缺血性脑卒中后不同阶段糖尿病小鼠皮质中的微RNA调控模式

缺血性中风后，microRNAs（miRNAs）参与各种过程，包括免疫反应、炎症和血管生成。糖尿病是增加缺血性脑卒中风险的一个关键因素；然而，miRNA 在糖尿病脑卒中不同阶段的调控模式仍不清楚。本研究全面分析了糖尿病小鼠在大脑中动脉闭塞（MCAO）后再灌注1、3和7天的miRNA表达谱。我们鉴定了糖尿病脑卒中中的差异表达（DE）miRNA，发现一些新型miRNA（novel_mir310、novel_mir89和novel_mir396）在脑卒中后出现了显著的失调。这些 DEmiRNAs 参与了细胞凋亡和紧密连接的形成。最后，我们发现了三组时间依赖性 DE miRNA（miR-6240、miR-135b-3p 和 miR-672-5p）。这些 miRNA 有可能成为糖尿病中风的生物标志物。这些发现为今后的研究提供了新的视角，强调了糖尿病脑卒中后 miRNA 表达的动态变化，为今后的临床应用提供了潜在的候选生物标志物。

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来源期刊

Journal of Molecular Neuroscience 医学-神经科学

CiteScore

6.60

自引率

3.20%

发文量

142

审稿时长

1 months

期刊介绍： The Journal of Molecular Neuroscience is committed to the rapid publication of original findings that increase our understanding of the molecular structure, function, and development of the nervous system. The criteria for acceptance of manuscripts will be scientific excellence, originality, and relevance to the field of molecular neuroscience. Manuscripts with clinical relevance are especially encouraged since the journal seeks to provide a means for accelerating the progression of basic research findings toward clinical utilization. All experiments described in the Journal of Molecular Neuroscience that involve the use of animal or human subjects must have been approved by the appropriate institutional review committee and conform to accepted ethical standards.