The Hematological Parameters of the Black Scorpionfish Scorpaena porcus Linnaeus, 1758 Under Experimental Hypothermia in vivo

IF 0.5 4区 生物学 Q4 MARINE & FRESHWATER BIOLOGY Russian Journal of Marine Biology Pub Date : 2024-04-15 DOI:10.1134/s1063074024010085
Yu. A. Silkin, E. N. Silkina, M. Yu. Silkin, V. E. Vasilets
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Abstract

The linear dimensions of erythrocytes, their nuclei, and the number of cells and hemoglobin in the blood of the black scorpionfish Scorpaena porcus Linnaeus, 1758 were studied in the autumn–winter period, at water temperatures of 17, 13, 8, and 4°С. The results showed high and relatively equal hemoglobin contents and the number of erythrocytes in the blood of black scorpionfish at 17 and 13°C. However, the studied parameters at a water temperature of 13°С were taken as the physiological norm, since this temperature more corresponded with the autumn temperatures of the Karadag coast. Lowering the water temperature in the aquarium to 8°C reduced the hemoglobin level by 25% and the number of erythrocytes by 26% in the blood of S. porcus. The linear dimensions of erythrocytes did not change when the temperature dropped to 8°C. At a temperature of 4°C the number of erythrocytes and hemoglobin in the blood of S. porcus rapidly decreased by 57% and by 31%, respectively, and swelling of erythrocytes was observed. The large diameter of red blood cells increased by 11% and the small diameter by 25%. The linear dimensions of the nuclei of erythrocytes increased by 12% along the major and minor axes at a temperature of 8°С. At 4°C, the nuclei of erythrocytes decreased in size, along the major axis by 7%, and along the minor axis by 9%, indicating a disruption of the chromatin structure in the nuclear apparatus of the cell. The results we obtained made it possible to carry out a clear gradation of the temperature resistance of S. porcus. At water temperatures below 8°С, destructive processes occurred in the blood, indicating the exhaustion of protective reserves in this species. The data we obtained can be used in fish mariculture as indicators of resistance to temperature factors.

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黑蝎子鱼(Scorpaena porcus Linnaeus, 1758)在实验性低体温条件下的体内血液学参数
摘要 研究了黑蝎鱼(Scorpaena porcus Linnaeus, 1758)在秋冬季节水温为17、13、8和4°С时血液中红细胞的线形尺寸、细胞核、细胞数和血红蛋白。结果表明,在 17 和 13 摄氏度时,黑蝎子鱼血液中的血红蛋白含量和红细胞数量较高且相对相等。不过,所研究的参数以水温为 13°С时的生理正常值为准,因为这一温度更符合卡拉达格海岸的秋季气温。将水族箱中的水温降至 8°C,会使猪笼草鱼血液中的血红蛋白含量降低 25%,红细胞数量减少 26%。温度降至 8°C 时,红细胞的线性尺寸没有变化。在温度为 4°C 时,孔雀石绿血液中的红细胞数量和血红蛋白分别迅速减少了 57% 和 31%,并观察到红细胞肿胀。红细胞的大直径增加了 11%,小直径增加了 25%。在温度为 8°С 时,红细胞核的线性尺寸沿主轴和次轴增加了 12%。在 4°C 时,红细胞核的尺寸沿主轴减小了 7%,沿次轴减小了 9%,这表明细胞核装置中的染色质结构发生了破坏。我们获得的结果使我们能够对猪蓝藻的耐温性进行清晰的分级。当水温低于 8°С 时,血液中会出现破坏过程,这表明该物种的保护储备已经耗尽。我们获得的数据可用于鱼类海水养殖,作为抵抗温度因素的指标。
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来源期刊
Russian Journal of Marine Biology
Russian Journal of Marine Biology 生物-海洋与淡水生物学
CiteScore
1.00
自引率
0.00%
发文量
46
审稿时长
6-12 weeks
期刊介绍: The Russian Journal of Marine Biology was founded in 1975 by Alexey V. Zhirmunsky, member of the Russian Academy of Sciences. The Russian Journal of Marine Biology covers a wide range of research and some applied aspects of marine biology as a synthetic science related to various fields of study on marine biota and environment. It presents fundamental research on biological processes at molecular, cellular, organismal, and populational levels in marine organisms. Consideration is given to marine objects as models in life sciences. The journal also publishes papers dedicated to events in Russian and international marine biological science and the history of biology.
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