Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

IF 1.3 4区 生物学 Q4 MICROBIOLOGY Microbiology Pub Date : 2024-04-02 DOI:10.1134/s0026261723604244
E. P. Efremova, O. M. Zemlyanko, G. A. Zhouravleva
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引用次数: 0

Abstract

Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.

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应用流式细胞仪检测酵母菌翻译终止因子突变体的活力
摘要 编码翻译终止因子的重要基因 SUP45 和 SUP35 的无义突变会影响酿酒酵母细胞的活力。流式细胞术显示,突变体的活力比野生型菌株低 3.5-4 倍。此外,突变体对超声波处理的敏感性更高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiology
Microbiology 生物-微生物学
CiteScore
2.40
自引率
13.30%
发文量
60
审稿时长
6-12 weeks
期刊介绍: Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.
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