Si–rhodamine derivative with a large stokes shift for ELISA-based detection of SARS-CoV-2†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL Sensors & diagnostics Pub Date : 2024-04-15 DOI:10.1039/D4SD00038B
Yan-Hong Liu, Hong Zhang, Kang-Kang Yu, Xiao-Fang Pei, Jia-Nan Xu, Shan-Yong Chen, Xiao-Qi Yu and Kun Li
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Abstract

In view of the worldwide impact of SARS-CoV-2, developing rapid and accurate ELISA-based methods for detecting SARS-CoV-2 is of great importance for diagnosing and controlling coronavirus disease 2019. Herein, we report highly stable and fluorescently bright Si–rhodamine analogues with obviously improved Stokes shifts for IgG antibody labelling. These new fluorescent dye labels provide a promising fluorescence tool for SARS-Cov-2 detection.

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具有大斯托克斯偏移的 Si-Rhodamine 衍生物,用于基于 ELISA 检测 SARS-CoV-2
开发基于酶联免疫吸附试验(ELISA)的快速、准确的 SARS-Cov-2 检测方法对疾病的诊断和控制具有极其重要的意义。在此,我们报告了一种高稳定性和高亮度的 Si-Rhodamine 类似物,其用于 IgG 抗体标记的斯托克斯位移有明显改善。这种新型标记荧光染料为 SARS-Cov-2 的检测提供了一种前景广阔的荧光工具。
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