Plant growth-promoting and biocontrol traits of endophytic Bacillus licheniformis against soft rot causing Pythium myriotylum in ginger plant

IF 3.5 4区 生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-04-05 DOI:10.1002/jobm.202300643
Khaloud Mohammed Alarjani, Mohamed S. Elshikh
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Abstract

Bacterial endophytes from plants harbor diverse metabolites that play major roles in biocontrol and improve plant growth. In this study, a total of 12 endophytic bacteria were isolated from the ginger rhizome. The strain K3 was highly effective in preventing mycelia growth of Pythium myriotylum (78.5 ± 1.5% inhibition) in dual culture. The cell-free extract (2.5%) of endophyte K3 inhibited 76.3 ± 4.8% mycelia growth, and 92.4 ± 4.2% inhibition was observed at a 5% sample concentration. The secondary metabolites produced by Bacillus licheniformis K3 showed maximum activity against Pseudomonas syringae (24 ± 1 mm zone of inhibition) and Xanthomonas campestris (28 ± 3 mm zone of inhibition). The strain K3 produced 28.3 ± 1.7 IU mL−1 protease, 28.3 ± 1.7 IU mL−1 cellulase, and 2.04 ± 0.13 IU mL−1 chitinase, respectively. The ginger rhizome treated with K3 in the greenhouse registered 53.8 ± 1.4% soft rot incidence, and the streptomycin-treated pot registered 78.3 ± 1.7% disease incidence. The selected endophyte K3 improved ascorbate peroxidase (1.37 ± 0.009 µmole ASC min−1 mg−1 protein), catalase (8.7 ± 0.28 µmole min−1 mg−1 protein), and phenylalanine ammonia-lyase (26.2 ± 0.99 Umg−1) in the greenhouse. In addition, K3 treatment in the field trial improved rhizome yield (730 ± 18.4 g) after 180 days (p < 0.01). The shoot length was 46 ± 8.3 cm in K3-treated plants, and it was about 31% higher than the control treatment (p < 0.01). The lytic enzyme-producing and growth-promoting endophyte is useful in sustainable crop production through the management of biotic stress.

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内生地衣芽孢杆菌对生姜软腐病病原菌 Pythium myriotylum 的植物生长促进和生物控制特性
植物中的细菌内生菌蕴藏着多种代谢产物,在生物防治和改善植物生长方面发挥着重要作用。本研究从生姜根茎中分离出了 12 种内生细菌。在双重培养中,菌株 K3 能有效阻止 Pythium myriotylum 菌丝生长(抑制率为 78.5 ± 1.5%)。内生菌 K3 的无细胞提取物(2.5%)对菌丝生长的抑制率为 76.3 ± 4.8%,5% 样品浓度下的抑制率为 92.4 ± 4.2%。地衣芽孢杆菌 K3 产生的次生代谢物对丁香假单胞菌(24 ± 1 mm 抑菌区)和野油菜黄单胞菌(28 ± 3 mm 抑菌区)表现出最大的活性。菌株 K3 分别产生 28.3 ± 1.7 IU mL-1 蛋白酶、28.3 ± 1.7 IU mL-1 纤维素酶和 2.04 ± 0.13 IU mL-1 几丁质酶。在温室中用 K3 处理的生姜根茎软腐病发病率为 53.8 ± 1.4%,而用链霉素处理的盆生姜根茎软腐病发病率为 78.3 ± 1.7%。在温室中,所选内生菌 K3 提高了抗坏血酸过氧化物酶(1.37 ± 0.009 µmole ASC min-1 mg-1 蛋白质)、过氧化氢酶(8.7 ± 0.28 µmole min-1 mg-1 蛋白质)和苯丙氨酸氨解酶(26.2 ± 0.99 Umg-1)。此外,田间试验中的 K3 处理提高了 180 天后的根茎产量(730 ± 18.4 克)(p < 0.01)。经 K3 处理的植株的芽长为 46 ± 8.3 厘米,比对照处理高出约 31%(p < 0.01)。产生溶菌酶和促进生长的内生菌可通过管理生物胁迫实现作物的可持续生产。
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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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