A cellulosomal yeast reaction system of lignin-degrading enzymes for cellulosic ethanol fermentation

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2024-04-12 DOI:10.1007/s10529-024-03485-0

Yutong Ye, Han Liu, Zhipeng Wang, Qi Qi, Jiliang Du, Shen Tian

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Abstract

Biofuel production from lignocellulose feedstocks is sustainable and environmentally friendly. However, the lignocellulosic pretreatment could produce fermentation inhibitors causing multiple stresses and low yield. Therefore, the engineering construction of highly resistant microorganisms is greatly significant. In this study, a composite functional chimeric cellulosome equipped with laccase, versatile peroxidase, and lytic polysaccharide monooxygenase was riveted on the surface of Saccharomyces cerevisiae to construct a novel yeast strain YI/LVP for synergistic lignin degradation and cellulosic ethanol production. The assembly of cellulosome was assayed by immunofluorescence microscopy and flow cytometry. During the whole process of fermentation, the maximum ethanol concentration and cellulose conversion of engineering strain YI/LVP reached 8.68 g/L and 83.41%, respectively. The results proved the availability of artificial chimeric cellulosome containing lignin-degradation enzymes for cellulosic ethanol production. The purpose of the study was to improve the inhibitor tolerance and fermentation performance of S. cerevisiae through the construction and optimization of a synergistic lignin-degrading enzyme system based on cellulosome.

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用于纤维素乙醇发酵的木质素降解酶的纤维素体酵母反应系统

利用木质纤维素原料生产生物燃料既可持续又环保。然而，木质纤维素预处理过程中会产生发酵抑制剂，造成多重压力和低产量。因此，工程构建高抗性微生物意义重大。本研究在酿酒酵母（Saccharomyces cerevisiae）表面铆接了具有漆酶、多功能过氧化物酶和裂解多糖单氧化酶的复合功能嵌合纤维素酶体，构建了新型酵母菌株YI/LVP，用于协同降解木质素和生产纤维素乙醇。免疫荧光显微镜和流式细胞仪检测了纤维素体的组装情况。在整个发酵过程中，工程菌株 YI/LVP 的最高乙醇浓度和纤维素转化率分别达到 8.68 g/L 和 83.41%。结果证明，含有木质素降解酶的人工嵌合纤维素体可用于纤维素乙醇的生产。该研究的目的是通过构建和优化基于纤维素体的协同木质素降解酶系统，提高 S. cerevisiae 的抑制剂耐受性和发酵性能。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.