Assessment of Damnacanthus major Siebold & Zucc callus for antioxidative and moisturizing capacities using an artificial skin alternative

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-04-13 DOI:10.1007/s12257-024-00103-8
Byung Man Lee, Hyun Ju Woo, Bum Jun Jang, Jae Ahn Shin, Young Min Ham, Eun Bi Jang, Sung Chun Kim, Jeong Mi Kim, Hwa Sung Shin
{"title":"Assessment of Damnacanthus major Siebold & Zucc callus for antioxidative and moisturizing capacities using an artificial skin alternative","authors":"Byung Man Lee, Hyun Ju Woo, Bum Jun Jang, Jae Ahn Shin, Young Min Ham, Eun Bi Jang, Sung Chun Kim, Jeong Mi Kim, Hwa Sung Shin","doi":"10.1007/s12257-024-00103-8","DOIUrl":null,"url":null,"abstract":"<p>Plants contain many useful substances; however, their availability and uniformity are constrained. Controlled cell culture is attracting attention as a method for identifying useful substances with better quality control than conventional plant extraction techniques. Artificial skin is widely used as an alternative to animal testing to evaluate the safety and efficacy of cosmetic materials. The <i>Damnacanthus major</i> Siebold &amp; Zucc is an evergreen shrub of the madder family, and the anthraquinone series substances present in its roots have antioxidant and whitening effects, implying its potential to be used in cosmetics. In this study, the antioxidant, moisturizing, and phototoxic properties of the ethanol extract of its callus were assessed using an artificial skin alternative. It showed a distinct antioxidant effect over 600 μg/mL and a moisturizing effect over 125 μg/mL. Phospho-extracellular signal-regulated kinase 1/2 and cAMP response element-binding protein were found to be upregulated in the artificial skin due to the moisturizing mechanism, and phospho-NFκB repressing factor was observed to be upregulated in human epidermal keratinocytes (HaCaT cells) due to the antioxidant mechanism. Additionally, analysis of the artificial skin revealed no phototoxicity up to 1 mg/mL. The results of this study demonstrate that the ethanol extract of <i>Damnacanthus major</i> Siebold &amp; Zucc can be used as a cosmetic material.</p>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Bioprocess Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12257-024-00103-8","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Plants contain many useful substances; however, their availability and uniformity are constrained. Controlled cell culture is attracting attention as a method for identifying useful substances with better quality control than conventional plant extraction techniques. Artificial skin is widely used as an alternative to animal testing to evaluate the safety and efficacy of cosmetic materials. The Damnacanthus major Siebold & Zucc is an evergreen shrub of the madder family, and the anthraquinone series substances present in its roots have antioxidant and whitening effects, implying its potential to be used in cosmetics. In this study, the antioxidant, moisturizing, and phototoxic properties of the ethanol extract of its callus were assessed using an artificial skin alternative. It showed a distinct antioxidant effect over 600 μg/mL and a moisturizing effect over 125 μg/mL. Phospho-extracellular signal-regulated kinase 1/2 and cAMP response element-binding protein were found to be upregulated in the artificial skin due to the moisturizing mechanism, and phospho-NFκB repressing factor was observed to be upregulated in human epidermal keratinocytes (HaCaT cells) due to the antioxidant mechanism. Additionally, analysis of the artificial skin revealed no phototoxicity up to 1 mg/mL. The results of this study demonstrate that the ethanol extract of Damnacanthus major Siebold & Zucc can be used as a cosmetic material.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
使用人工皮肤替代品评估大芒萁(Damnacanthus major Siebold & Zucc)胼胝的抗氧化和保湿能力
植物中含有许多有用的物质,但这些物质的可用性和均匀性受到限制。与传统的植物提取技术相比,受控细胞培养作为一种能更好地控制质量、识别有用物质的方法正备受关注。人造皮肤被广泛用于替代动物试验,以评估化妆品材料的安全性和有效性。Damnacanthus major Siebold & Zucc 是一种茜草科常绿灌木,其根部含有的蒽醌系列物质具有抗氧化和美白作用,这意味着它有潜力用于化妆品。本研究使用人工皮肤替代物评估了其胼胝体乙醇提取物的抗氧化、保湿和光毒性特性。结果表明,超过 600 μg/mL 的乙醇提取物具有明显的抗氧化效果,超过 125 μg/mL 的乙醇提取物具有保湿效果。研究发现,由于保湿机制,细胞外信号调节激酶 1/2磷酸化和 cAMP 反应元件结合蛋白在人造皮肤中上调,而由于抗氧化机制,在人类表皮角质细胞(HaCaT 细胞)中观察到 NFκB 抑制因子磷酸化上调。此外,对人造皮肤的分析表明,在 1 毫克/毫升的浓度下没有光毒性。这项研究结果表明,Damnacanthus major Siebold & Zucc 的乙醇提取物可用作化妆品材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
期刊最新文献
Assessing the applicability of tunicate skin-extracted cellulose as a base material for ultrasound gel Fabrication of protein–inorganic biohybrid as an imageable drug delivery system comprising transferrin, green fluorescent protein, and copper phosphate Continuous cell recycling in methylotrophic yeast Pichia pastoris to enhance product yields: a case study with Yarrowia lipolytica lipase Lip2 Sensitive detection of SARS-CoV2 spike antibodies by a paper-based polypyrrole/reduced graphene oxide sensor A neural ordinary differential equation model for predicting the growth of Chinese Hamster Ovary cell in a bioreactor system
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1