A new glucosyltransferase UGT78 from Iris sanguinea is a putative negative regulator in cadmium stress response

Abstract

Urbanization has resulted in a significant degradation of soil quality, subjecting plants to persistent abiotic stressors such as heavy metal pollution, salinization, and drought. UDP-glycosyltransferases (UGTs) participate in protein glycosylation, secondary metabolite synthesis, and detoxification of exogenous toxic substances. Iris sanguinea Donn ex Hornem exhibits a high degree of resistance to various abiotic stressors. To enhance the plant’s response to adversity, a novel glycosyltransferase belonging to the UGT78 family, encoding flavonoid 3-O-glucosyltransferase (UF3GT), was cloned from the monocot species I. sanguinea. Compared with the control group, overexpression of IsUGT78 enhanced sensitivity to cadmium stress, while showing no significant impact under NaCl and d-sorbitol treatments. Under cadmium treatment, arabidopsis exogenously transformed with the IsUGT78 gene possessed lower germination, fresh weight, root length, and chlorophyll content and increased malondialdehyde content than the wild type arabidopsis. In addition, metabolomics in leaves led to the identification of 299 flavonoid metabolites, eight and 127 which were significantly up- and down-regulated, respectively, in the transgenic plants. Of note, all eight upregulated flavonoid compounds were glycosylated. Given that arabidopsis, which exogenously expresses the IsUGT78 gene, has reduced resistance to cadmium, IsUGT78 may lead to a reduced ability to cope with cadmium stress.