Preparation of PLCL/ECM nerve conduits by electrostatic spinning technique and evaluation in vitro and in vivo

IF 3.7 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of neural engineering Pub Date : 2024-04-04 DOI:10.1088/1741-2552/ad3851
Yizhan Ma, Runze Zhang, Xiaoyan Mao, Xiaoming Li, Ting Li, Fang Liang, Jing He, Lili Wen, Weizuo Wang, Xiao Li, Yanhui Zhang, Honghao Yu, Binhan Lu, Tianhao Yu, Qiang Ao
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Abstract

Objective. Artificial nerve scaffolds composed of polymers have attracted great attention as an alternative for autologous nerve grafts recently. Due to their poor bioactivity, satisfactory nerve repair could not be achieved. To solve this problem, we introduced extracellular matrix (ECM) to optimize the materials. Approach. In this study, the ECM extracted from porcine nerves was mixed with Poly(L-Lactide-co-ϵ-caprolactone) (PLCL), and the innovative PLCL/ECM nerve repair conduits were prepared by electrostatic spinning technology. The novel conduits were characterized by scanning electron microscopy (SEM), tensile properties, and suture retention strength test for micromorphology and mechanical strength. The biosafety and biocompatibility of PLCL/ECM nerve conduits were evaluated by cytotoxicity assay with Mouse fibroblast cells and cell adhesion assay with RSC 96 cells, and the effects of PLCL/ECM nerve conduits on the gene expression in Schwann cells was analyzed by real-time polymerase chain reaction (RT-PCR). Moreover, a 10 mm rat (Male Wistar rat) sciatic defect was bridged with a PLCL/ECM nerve conduit, and nerve regeneration was evaluated by walking track, mid-shank circumference, electrophysiology, and histomorphology analyses. Main results. The results showed that PLCL/ECM conduits have similar microstructure and mechanical strength compared with PLCL conduits. The cytotoxicity assay demonstrates better biosafety and biocompatibility of PLCL/ECM nerve conduits. And the cell adhesion assay further verifies that the addition of ECM is more beneficial to cell adhesion and proliferation. RT-PCR showed that the PLCL/ECM nerve conduit was more favorable to the gene expression of functional proteins of Schwann cells. The in vivo results indicated that PLCL/ECM nerve conduits possess excellent biocompatibility and exhibit a superior capacity to promote peripheral nerve repair. Significance. The addition of ECM significantly improved the biocompatibility and bioactivity of PLCL, while the PLCL/ECM nerve conduit gained the appropriate mechanical strength from PLCL, which has great potential for clinical repair of peripheral nerve injuries.
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利用静电纺丝技术制备 PLCL/ECM 神经导管并进行体内外评估
目的。最近,由聚合物组成的人工神经支架作为自体神经移植的替代品引起了广泛关注。由于其生物活性较差,无法实现令人满意的神经修复效果。为了解决这个问题,我们引入了细胞外基质(ECM)来优化材料。方法。本研究将从猪神经中提取的 ECM 与聚(L-乳酸-共ϵ-己内酯)(PCLL)混合,通过静电纺丝技术制备了 PLCL/ECM 创新型神经修复导管。通过扫描电子显微镜(SEM)、拉伸性能和缝合强度测试对新型导管的微观形态和机械强度进行了表征。小鼠成纤维细胞细胞毒性实验和 RSC 96 细胞粘附实验评估了 PLCL/ECM 神经导管的生物安全性和生物相容性,实时聚合酶链反应(RT-PCR)分析了 PLCL/ECM 神经导管对许旺细胞基因表达的影响。此外,大鼠(雄性 Wistar 大鼠)坐骨神经缺损 10 mm,用 PLCL/ECM 神经导管进行桥接,并通过行走轨迹、胫骨中段周长、电生理学和组织形态学分析评估神经再生情况。主要结果。结果显示,与 PLCL 导管相比,PLCL/ECM 导管具有相似的微观结构和机械强度。细胞毒性试验表明 PLCL/ECM 神经导管具有更好的生物安全性和生物相容性。细胞粘附试验进一步验证了添加 ECM 更有利于细胞粘附和增殖。RT-PCR 显示,PLCL/ECM 神经导管更有利于许旺细胞功能蛋白的基因表达。体内研究结果表明,PLCL/ECM 神经导管具有良好的生物相容性,在促进周围神经修复方面表现出卓越的能力。意义重大。ECM 的加入明显改善了 PLCL 的生物相容性和生物活性,同时 PLCL/ECM 神经导管从 PLCL 中获得了适当的机械强度,在临床修复周围神经损伤方面具有巨大潜力。
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来源期刊
Journal of neural engineering
Journal of neural engineering 工程技术-工程:生物医学
CiteScore
7.80
自引率
12.50%
发文量
319
审稿时长
4.2 months
期刊介绍: The goal of Journal of Neural Engineering (JNE) is to act as a forum for the interdisciplinary field of neural engineering where neuroscientists, neurobiologists and engineers can publish their work in one periodical that bridges the gap between neuroscience and engineering. The journal publishes articles in the field of neural engineering at the molecular, cellular and systems levels. The scope of the journal encompasses experimental, computational, theoretical, clinical and applied aspects of: Innovative neurotechnology; Brain-machine (computer) interface; Neural interfacing; Bioelectronic medicines; Neuromodulation; Neural prostheses; Neural control; Neuro-rehabilitation; Neurorobotics; Optical neural engineering; Neural circuits: artificial & biological; Neuromorphic engineering; Neural tissue regeneration; Neural signal processing; Theoretical and computational neuroscience; Systems neuroscience; Translational neuroscience; Neuroimaging.
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