Proposal of Helicobacter higonensis sp. nov. isolated from a human clinical specimen, and emended description of Helicobacter valdiviensis Collado, 2014

IF 1.9 4区 医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2024-04-10 DOI:10.1111/1348-0421.13127
Junko Tomida, Tohru Miyoshi-Akiyama, Ryo Kutsuna, Hiroyasu Tsutsuki, Tomohiro Sawa, Margo Cnockaert, Peter Vandamme, Yoshiaki Kawamura
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Abstract

We have previously isolated a gram-negative microaerophilic strain, PAGU2000T from a patient presenting with a fever in Kumamoto Prefecture, Japan. The present study aimed to comprehensively analyze the taxonomy of the isolated strain using a polyphasic approach. The 16S rRNA gene sequence analysis indicated that the strain was a member of enterohepatic Helicobacter. The strain PAGU2000T shared a 97.5% 16S rRNA gene nucleotide identity with Helicobacter valdiviensis, and this taxonomic position was confirmed by phylogenetic analysis of the GyrA amino acid sequences. The proposed strain PAGU2000T has a 1.482 Mbp chromosome with a DNA G + C content of 31.3 mol% and encodes 1520 predicted coding sequences. The average nucleotide identity between the strain PAGU2000T and type strain of H. valdiviensis was 70.3%, which was lower than the recommended threshold of 95% for species delineation. The strain PAGU2000T was a motile, non-spore-forming, and spiral-shaped bacterium, exhibiting catalase and oxidase activities but not urease and nitrate reduction. This study demonstrates that the isolate represents a novel species within enterohepatic Helicobacter, for which the name Helicobacter higonensis is proposed (type strain: PAGU2000T = GTC 16811T = LMG 33095T). In this study, we describe the phenotypic and morphological features of this strain and propose an emended description of some biochemical traits of H. valdiviensis.

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从人类临床标本中分离出新的Helicobacter higonensis sp.的建议,以及对Helicobacter valdiviensis Collado, 2014的修订描述
我们曾从日本熊本县的一名发烧患者身上分离出一株嗜微气的革兰氏阴性菌 PAGU2000T。本研究旨在采用多相法全面分析分离菌株的分类。16S rRNA 基因序列分析表明,该菌株属于肠肝螺旋杆菌。菌株 PAGU2000T 与瓦尔迪维氏螺旋杆菌的 16S rRNA 基因核苷酸相同度为 97.5%,GyrA 氨基酸序列的系统进化分析证实了这一分类学定位。拟议的菌株 PAGU2000T 的染色体为 1.482 Mbp,DNA G + C 含量为 31.3 mol%,编码 1520 个预测的编码序列。菌株 PAGU2000T 与 H. valdiviensis 型菌株的平均核苷酸同一性为 70.3%,低于建议的 95% 的物种划分阈值。菌株 PAGU2000T 是一种能运动、不形成孢子、呈螺旋状的细菌,具有过氧化氢酶和氧化酶活性,但不具有脲酶和硝酸盐还原活性。这项研究表明,该分离菌株代表了肠肝螺旋杆菌中的一个新物种,并将其命名为希贡螺旋杆菌(模式菌株:PAGU2000T = GTC 16811T = LMG 33095T)。在本研究中,我们描述了该菌株的表型和形态特征,并提出了对 H. valdiviensis 某些生化特征的修正描述。
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来源期刊
Microbiology and Immunology
Microbiology and Immunology 医学-免疫学
CiteScore
5.20
自引率
3.80%
发文量
78
审稿时长
1 months
期刊介绍: Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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