Genome-wide identification and expression analysis of the ALDH gene family and functional analysis of PaALDH17 in Prunus avium

IF 3.4 3区生物学 Q1 PLANT SCIENCES Physiology and Molecular Biology of Plants Pub Date : 2024-04-06 DOI:10.1007/s12298-024-01444-7

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Abstract

ALDH (Aldehyde dehydrogenase), as an enzyme that encodes the dehydroxidization of aldehydes into corresponding carboxylic acids, played an important role inregulating gene expression in response to many kinds of biotic and abiotic stress, including saline–alkali stress. Saline–alkali stress was a common stress that seriously affected plant growth and productivity. Saline–alkali soil contained the characteristics of high salinity and high pH value, which could cause comprehensive damage such as osmotic stress, ion toxicity, high pH, and HCO³⁻/CO₃²⁻ stress. In our study, 18 PaALDH genes were identified in sweet cherry genome, and their gene structures, phylogenetic analysis, chromosome localization, and promoter cis-acting elements were analyzed. Quantitative real-time PCR confirmed that PaALDH17 exhibited the highest expression compared to other members under saline–alkali stress. Subsequently, it was isolated from Prunus avium, and transgenic A. thaliana was successfully obtained. Compared with wild type, transgenic PaALDH17 plants grew better under saline–alkali stress and showed higher chlorophyll content, Superoxide dismutase (SOD), Peroxidase (POD) and Catalase (CAT) enzyme activities, which indicated that they had strong resistance to stress. These results indicated that PaALDH17 improved the resistance of sweet cherries to saline–alkali stress, which in turn improved quality and yields.

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李子中 ALDH 基因家族的全基因组鉴定和表达分析以及 PaALDH17 的功能分析

摘要 ALDH（醛脱氢酶）是一种编码将醛脱羟化为相应羧酸的酶，在应对包括盐碱胁迫在内的多种生物和非生物胁迫时对基因表达起着重要的调控作用。盐碱胁迫是严重影响植物生长和生产力的常见胁迫。盐碱地具有高盐度和高pH值的特点，可造成渗透胁迫、离子毒性、高pH值和HCO3-/CO32-胁迫等综合伤害。我们的研究在甜樱桃基因组中发现了 18 个 PaALDH 基因，并对其基因结构、系统进化分析、染色体定位和启动子顺式作用元件进行了分析。实时定量 PCR 证实，与其他成员相比，PaALDH17 在盐碱胁迫下的表达量最高。随后，研究人员从梅花中分离出该基因，并成功获得了转基因A.与野生型相比，转基因 PaALDH17 植物在盐碱胁迫下生长更好，叶绿素含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）酶活性更高，表明其具有较强的抗胁迫能力。这些结果表明，PaALDH17 提高了甜樱桃对盐碱胁迫的抗性，从而改善了品质和产量。

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来源期刊

Physiology and Molecular Biology of Plants PLANT SCIENCES-

CiteScore

7.10

自引率

0.00%

发文量

126

期刊介绍： Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.