Prokaryotic Communities during the Self-Overgrowing of a Chemical Industry Waste Storage Facility

IF 0.7 4区 环境科学与生态学 Q4 ECOLOGY Contemporary Problems of Ecology Pub Date : 2024-04-09 DOI:10.1134/s1995425524020112
I. G. Shirokikh, N. A. Bokov, E. V. Dabakh, L. V. Kondakova, A. A. Shirokikh, T. Ya. Ashikhmina
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Abstract

High-performance sequencing using the Illumina technology has been used to investigate the diversity of prokaryotes in three soil samples (Technosols) selected on the territory of the former tailings of liquid waste of chemical production near the city of Kirovo-Chepetsk, Kirov oblast. The taxonomic richness and phylogenetic diversity of microbial communities formed in the process of regenerative succession of the technogenic landscape, in comparison with alluvial soil (Fluvisols)—a natural analogue of the one that was disturbed as a result of the disposal of waste from a chemical enterprise—is assessed. The analysis of the indicators of taxonomic richness and diversity demonstrate their lower values in the soils formed on waste in comparison with the natural soil of the background site. Twenty-six bacterial and 2 archaeal phyla of prokaryotes are identified, accounting for more than 95% of the number of classified sequences. The phylum Actinobacteria (22–41%) and Proteobacteria (20–26%) dominate. At the level of higher taxa in disturbed soils, compared with the community of natural alluvial soil, trends in the representation of phylum are revealed: a decrease in the proportion of Verrucomicrobia and an increase in the proportion of Cyanobacteria. The greatest differences between the communities of technosols and background soil are revealed at the levels of orders, families, and genera. Common and unique genera have been identified for each of the four microbiomes. The detected differences in the composition and structure of the prokaryotic component of microbial communities of disturbed soils are related to the complex of their physicochemical properties (granulometric composition, pH of soil solution, Corg content, composition of toxicants of industrial origin, humidity regime, etc.) and the nature of vegetation cover. The results are of interest for the development of new approaches in future studies of the relationship of soil microbiota with ecosystem changes caused by human activity.

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化学工业废物储存设施自我过度生长过程中的原核生物群落
摘要 利用 Illumina 技术进行高性能测序,研究了基洛夫州基洛夫-切佩茨克市附近原化工生产液体废物尾矿地区选取的三个土壤样本(Technosols)中原核生物的多样性。与冲积土壤(Fluvisols)--因化工企业处理废物而受到干扰的自然类比土壤--相比,对技术景观再生演替过程中形成的微生物群落的分类丰富度和系统发育多样性进行了评估。对分类丰富度和多样性指标的分析表明,与背景场地的天然土壤相比,废物形成的土壤中的分类丰富度和多样性指标值较低。共鉴定出 26 个细菌门和 2 个古细菌门的原核生物,占分类序列数的 95% 以上。放线菌门(22-41%)和变形菌门(20-26%)占主导地位。与自然冲积土壤群落相比,扰动土壤中的高级分类群落显示出门类代表的趋势:蛭弧菌比例下降,蓝藻比例上升。技术溶胶群落与背景土壤群落之间的最大差异体现在目、科和属的层次上。四个微生物群落中的每个群落都确定了常见和独特的属。扰动土壤微生物群落中原核生物成分组成和结构的差异与土壤的物理化学性质(颗粒度组成、土壤溶液 pH 值、Corg 含量、工业毒物成分、湿度等)和植被覆盖性质的复杂性有关。这些结果有助于在今后研究土壤微生物群与人类活动造成的生态系统变化之间的关系时开发新的方法。
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来源期刊
Contemporary Problems of Ecology
Contemporary Problems of Ecology 环境科学-生态学
CiteScore
1.40
自引率
12.50%
发文量
54
审稿时长
6-12 weeks
期刊介绍: Contemporary Problems of Ecology is a multidisciplinary periodical that publishes original works on the following subjects: theoretical and methodical issues of ecology, regional aspects of ecology, regional ecological disasters, structure and functioning of ecosystems, anthropogenic transformation of ecosystems. All basic aspects of modern ecology, including the most complicated interactions between living organisms and their environment, are presented. Some of the journal issues are dedicated to global changes in biological diversity at various levels of organization (populations, species, ecosystems) principles and methods of nature conservation.
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