Construction and validation of key genes-related prognosis model in children with acute myeloid leukaemia

IF 2.2 4区医学 Q3 HEMATOLOGY International Journal of Laboratory Hematology Pub Date : 2024-04-10 DOI:10.1111/ijlh.14272

Fan Huang, Chuan Ming, Yuqian Jiang, Chenli Li, Cheng Tan

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Abstract

Introduction

To identify the differentially expressed genes of acute myeloid leukaemia (AML) and construct and verify a survival prognosis model combined with patient survival information.

Methods

The TARGET database was searched to identify differentially expressed peripheral blood genes in children with AML and healthy children. A gene set functional analysis and pathway analysis were performed using gene ontology and the KEGG pathway. A prognostic model for children with AML was constructed using univariate Cox, LASSO Cox regression and multivariate Cox regression analyses. Time-dependent receiver operating characteristic (ROC) curves were adopted to assess the predictive capacity of the prognostic models.

Results

In total, 1640 differentially expressed genes were screened (1119 upregulated and 521 downregulated genes). The differentially expressed genes were mainly involved in nutrient metabolism and cytochrome P450 metabolism. Six key genes related to the prognosis of AML, FAM157A, GPR78, IRX5, RP4-800G7.1, RP11-179H18.5 and RP11-61N20.3, were identified. Kaplan–Meier curves indicated that 3-year and 5-year overall survival was significantly higher in the low-risk group than in the high-risk group. The area under the ROC curve was 0.722. At different stages of AML, FAM157A and RP4-800G7.1 exhibited significant differences in expression. The expression levels of FAM157A were significantly decreased in AML, whereas the expression levels of GPR78, IRX5, RP4-800G7.1, RP11-179H18.5 and RP11-61N20.3 were significantly increased in AML.

Conclusion

A prognosis-related gene model of AML was successfully constructed, and the expression levels of the model genes varied with AML stage.

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构建和验证急性髓性白血病儿童关键基因相关预后模型

方法检索TARGET数据库，确定急性髓性白血病（AML）患儿和健康儿童外周血中差异表达的基因。利用基因本体论和 KEGG 通路进行了基因组功能分析和通路分析。通过单变量 Cox、LASSO Cox 回归和多变量 Cox 回归分析，建立了急性髓细胞性白血病患儿的预后模型。结果共筛选出1640个差异表达基因（1119个上调基因和521个下调基因）。差异表达基因主要涉及营养代谢和细胞色素 P450 代谢。研究发现了六个与急性髓细胞性白血病预后相关的关键基因：FAM157A、GPR78、IRX5、RP4-800G7.1、RP11-179H18.5和RP11-61N20.3。Kaplan-Meier 曲线显示，低风险组的 3 年和 5 年总生存率明显高于高风险组。ROC 曲线下的面积为 0.722。在急性髓细胞性白血病的不同阶段，FAM157A和RP4-800G7.1的表达有显著差异。结论成功构建了急性髓细胞性白血病预后相关基因模型，模型基因的表达水平随急性髓细胞性白血病分期而变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.