Fabrication of folic acid-embedded aminated drug encapsulated zeolitic imidazolate framework as promising drug delivery system for lung cancer

IF 2.7 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Journal of Materials Research Pub Date : 2024-04-05 DOI:10.1557/s43578-024-01328-2

Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Mohankumar Ramar, Palanisamy Arulselvan, Sridhar Muthusami

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Abstract

A novel pH-responsive drug carrier for the delivery of specific Docetaxel (DTX) administration is developed based on a zeolitic imidazolate framework (ZIF-8). Aminating the surface of ZIF-8 is allowed for the conjugation of folic acid (FA). Several spectroscopic studies characterized the newly fabricated DTX-encapsulated folic acid-embedded ethylene diamine (ED) ZIF-8 nanocomposites (DTX/FA@ED-ZIF-8). It has excellent chemical stability and high drug-loading efficiency. DTX from the folic acid-embedded aminated ZIF-8 (FA@ED-ZIF-8) is three-fold more efficient under acidic pH (5.0) than in physiological settings (pH 7.4), according to in vitro drug release tests. DTX/FA@ED-ZIF-8 exhibited cytotoxicity of 76.8% in an MTT experiment conducted in A549 and H1299 cells. Cell morphological and nuclear staining were investigated in the fabricated samples to support the MTT experiments. Further, the apoptosis mode of cell death was examined using Annexin V-FITC and PI by flow cytometry. These findings indicate that FA@ED-ZIF-8 holds great potential as a drug carrier for precise dosing.

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制备叶酸嵌入式胺化药物包封沸石咪唑盐框架，作为治疗肺癌的有效给药系统

以沸石咪唑酸盐框架（ZIF-8）为基础，开发出了一种新型 pH 值响应型药物载体，用于多西他赛（DTX）的特异性给药。对 ZIF-8 的表面进行氨基化处理后，叶酸（FA）就可以与之共轭。几项光谱研究表明，新制备的 DTX 包裹叶酸嵌入乙二胺（ED）ZIF-8 纳米复合材料（DTX/FA@ED-ZIF-8）具有优异的化学稳定性和高药效。它具有优异的化学稳定性和较高的药物负载效率。根据体外药物释放测试，在酸性 pH 值（5.0）条件下，嵌入叶酸的胺化 ZIF-8（FA@ED-ZIF-8）的 DTX 药物释放效率是生理环境（pH 值 7.4）条件下的三倍。在对 A549 和 H1299 细胞进行的 MTT 实验中，DTX/FA@ED-ZIF-8 的细胞毒性为 76.8%。对制备的样品进行了细胞形态学和细胞核染色研究，以支持 MTT 实验。此外，还通过流式细胞术使用 Annexin V-FITC 和 PI 对细胞凋亡模式进行了检测。这些研究结果表明，FA@ED-ZIF-8 具有作为药物载体进行精确给药的巨大潜力。

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来源期刊

Journal of Materials Research 工程技术-材料科学：综合

CiteScore

4.50

自引率

3.70%

发文量

362

审稿时长

2.8 months

期刊介绍： Journal of Materials Research (JMR) publishes the latest advances about the creation of new materials and materials with novel functionalities, fundamental understanding of processes that control the response of materials, and development of materials with significant performance improvements relative to state of the art materials. JMR welcomes papers that highlight novel processing techniques, the application and development of new analytical tools, and interpretation of fundamental materials science to achieve enhanced materials properties and uses. Materials research papers in the following topical areas are welcome. • Novel materials discovery • Electronic, photonic and magnetic materials • Energy Conversion and storage materials • New thermal and structural materials • Soft materials • Biomaterials and related topics • Nanoscale science and technology • Advances in materials characterization methods and techniques • Computational materials science, modeling and theory