A Study of the Effect of Glycodelin on the Level of T-Regulatory Lymphocytes and Acute Phase Proteins in Wistar Rats during the Administration of Allogenic Bone Marrow Cells

Q4 Biochemistry, Genetics and Molecular Biology Cell and Tissue Biology Pub Date : 2024-04-15 DOI:10.1134/s1990519x24010103

S. A. Zamorina, M. S. Bochkova, V. P. Timganova, S. V. Uzhviyuk, K. Yu. Shardina, V. V. Vlasova, M. B. Rayev

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Abstract

The amniotic variant of glycodelin (GdA) has pronounced immunomodulatory properties, being involved in the formation of immune tolerance during pregnancy. The effect of GdA on the amount of T-regulatory lymphocytes (Treg) and the level of acute phase proteins (α-2-macroglobulin (α-2M), orosomucoid, C-reactive protein (CRP)) were studied during the administration of allogeneic red bone marrow (BM) cells to Wistar rats in a dynamic in vivo experiment. It was established that the administration of GdA against the background of the administration of allogeneic BM caused an increase in the portion of peripheral Tregs among CD4⁺ lymphocytes at the end of the experiment (on the 21st day) as compared with the group, which received BM. It was demonstrated that GdA decreased the level of CRP and α-2M, but increased the orosomucoid concentration in the serum of experimental animals at the beginning of the experiment (third day); however, normalization of the content of acute phase proteins to the level of intact animals was observed by the end of the experiment (21st day) in all groups of experimental animals. Thus, glycodelin is able to realize an immunosuppressive effect relative to the allogeneic cells through an increase in the level of Treg and orosomucoid, as well as a decrease in the concentration of CRP and α-2M.

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甘油三酯对给予异基因骨髓细胞期间 Wistar 大鼠体内 T 调节淋巴细胞和急性期蛋白水平的影响研究

摘要羊膜变体甘油三酯（GdA）具有明显的免疫调节特性，参与妊娠期免疫耐受的形成。在给 Wistar 大鼠注射同种异体红骨髓（BM）细胞的动态体内实验中，研究了 GdA 对 T 调节淋巴细胞（Treg）数量和急性期蛋白（α-2-巨球蛋白（α-2M）、口服类粘蛋白、C 反应蛋白（CRP））水平的影响。结果表明，与接受同种异体红骨髓细胞的组别相比，在给予同种异体红骨髓细胞的背景下给予 GdA 会在实验结束时（第 21 天）增加 CD4+ 淋巴细胞中外周集落细胞的比例。实验结果表明，在实验开始时（第三天），GdA 降低了实验动物血清中 CRP 和 α-2M 的水平，但增加了口腔黏液的浓度；然而，在实验结束时（第 21 天），所有实验组动物的急性期蛋白含量都恢复到了完整动物的正常水平。因此，相对于同种异体细胞，甘橘色素能够通过提高 Treg 和 orosomucoid 的水平以及降低 CRP 和 α-2M 的浓度来实现免疫抑制效果。

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来源期刊

Cell and Tissue Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

0.80

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0.00%

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期刊介绍： The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.