The Cytokine Profile of Myocardial Cells with Coronary Heart Disease and Ischemic Cardiomyopathy

Q4 Biochemistry, Genetics and Molecular Biology Cell and Tissue Biology Pub Date : 2024-04-15 DOI:10.1134/s1990519x24010097

A. I. Stelmashenko, S. L. Andreev, L. S. Litvinova, V. V. Malashchenko, N. M. Todosenko, N. D. Gazatova, I. A. Khlusov, V. M. Shipulin

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Abstract

In this work, a comparative analysis of the myocardial cytokine profile was carried out in patients with coronary heart disease (CHD) and in patients with ischemic cardiomyopathy (ICM) against the background of CHD. The concentrations of 41 cytokines secreted by a 24-h tissue culture of the myocardium, intraoperatively collected from the right atrial (RA) appendage (control) and a peri-infarct zone (PZ) of the left ventricle (LV), were determined by the flow fluorimetry method using a multiplex test system. The aim of the work was to study in vitro the cytokine profile of myocardial cells to search for possible predictors of unfavorable outcomes of the surgical treatment of patients with CHD and ICM. The myocardial secretion of the pro-inflammatory molecules GM-CSF and IFN-γ increased significantly in patients with ICM against the background of CHD (up to 78–80 pg/g, p < 0.05) as compared with zero values in the case of CHD. At the same time, a threefold decrease in the concentration of the Fractalkine 3 ligand (Flt-3L, fms-like tyrosine kinase 3 ligand) was observed. A decrease in the secretion of Flt-3L was noted namely in the PZ–LV as compare with the RA appendage. In addition, the concentrations of fibroblast growth factor-2 (FGF-2), platelet-derived growth factor AB/BB (PDGF-AB/BB), interleukins IL-15 and IL-4, as well as regulated upon activation, normal T cell expressed and secreted (RANTES/CCL5), sharply decreased in the tissue myocardial PZ–LV culture as compared with RA appendage. The detected changes are analyzed using correlation and regression analyses; possible predictors of the risk of surgical treatment of patients from two groups are suggested. Pro-inflammatory cytokines (IL-5, IL-6) and chemokines (Flt-3L, IL-8), as well as factors of angiogenesis (VEGF) and angiostasis (IP-10), are suggested to be considered as potential markers of unfavorable outcome of the surgical treatment of cardiovascular diseases.

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冠心病和缺血性心肌病心肌细胞的细胞因子图谱

摘要在这项工作中，对冠心病（CHD）患者和以冠心病为背景的缺血性心肌病（ICM）患者的心肌细胞因子谱进行了比较分析。在术中从右心房（RA）阑尾（对照组）和左心室（LV）梗死周围（PZ）采集的心肌组织培养物中，通过使用多重测试系统的流动荧光测定法测定了 41 种细胞因子的分泌浓度。这项工作的目的是在体外研究心肌细胞的细胞因子谱，以寻找可能预测先天性心脏病和 ICM 患者手术治疗不良后果的因素。与患有心脏病的患者相比，ICM 患者心肌促炎分子 GM-CSF 和 IFN-γ 的分泌量明显增加（高达 78-80 pg/g，p <0.05）。同时，还观察到 Fractalkine 3 配体（Flt-3L，fms 样酪氨酸激酶 3 配体）的浓度下降了三倍。与 RA 附肢相比，PZ-LV 的 Flt-3L 分泌明显减少。此外，与 RA 附件相比，成纤维细胞生长因子-2（FGF-2）、血小板衍生生长因子 AB/BB（PDGF-AB/BB）、白细胞介素 IL-15 和 IL-4 以及正常 T 细胞表达和分泌的活化调节因子（RANTES/CCL5）在组织心肌 PZ-LV 培养物中的浓度急剧下降。利用相关性和回归分析对检测到的变化进行了分析，并提出了两组患者手术治疗风险的可能预测因素。建议将促炎细胞因子（IL-5、IL-6）和趋化因子（Flt-3L、IL-8）以及血管生成因子（VEGF）和血管止血因子（IP-10）视为心血管疾病手术治疗不利结果的潜在标志物。

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来源期刊

Cell and Tissue Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

0.80

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0.00%

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期刊介绍： The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.