Expression of serotonergic (5HT) receptors in patients with chronic rhinosinusitis with nasal polyposis and in normal nasal mucosa

Ahmed Osama Tolba Keshk, Sabry Magdy Sabry, Samia Ahmed Fawaz, Manal Ibrahim Salman, Heba Mahmoud Abd El-Megeed
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Abstract

Abstract Expression of serotonergic (5HT) receptors in patients with chronic rhinosinusitis with nasal polyposis and in normal nasal mucosa: a case-control study. Background Chronic rhinosinusitis with nasal polyposis is one of the challenging conditions regarding treatment and management. Many recent studies introduced new modalities for treatment like targeted immunomodulating drugs rather than antihistamines, local and systemic steroids, and endoscopic sinus surgery. Understanding the pathology behind the disease by studying its immunologic pathways and substances that take part in the inflammatory process can help in the introduction of new treatment options that can improve outcomes and decrease the costs of treatment. Objective The aim of our study is to demonstrate the presence of 5-HT receptors in nasal polyps and inferior turbinate mucosa in patients with chronic rhinosinusitis with nasal polyposis and then compare this expression of 5-HT receptors with inferior turbinate mucosa of the normal control group. Methods A case-control study was conducted on 80 patients. Specimens from nasal polypi and turbinate mucosa of patients with chronic rhinosinusitis (cases) and specimens from normal turbinate mucosa (controls) were stained with serotonin receptor Immunohistochemical staining by automated immunostainer in the pathology department laboratory in Ain Shams University Hospital with ratio 1:200–400 as recommended by the manufacturing company. Then, compared as regards stain distribution and intensity of mucosal lining, glands, and blood vessels. Results There was a significant difference between polyp cases and controls as regards lining epithelium and mucosal gland stain distribution and stain intensity. Also significant difference between the polyp cases and controls as regards blood vessel strain distribution. However, no significant difference was found as regards blood vessel stain intensity. There was a significant difference between turbinate cases and controls as regards lining epithelium stain distribution, and stain intensity. There was no significant difference between turbinate cases and controls as regards gland stain distribution. However, a significant difference was found between turbinate and controls as regards gland stain intensity. There was a significant difference between turbinate cases and controls as regards blood vessel stain distribution; however, no significant difference was present as regards stain intensity. There was no significant difference between polyp and turbinate cases as regards lining epithelium and blood vessel stain distribution and stain intensity. There was no significant difference between polyp and turbinate cases as regards gland stain distribution; however, a highly significant difference between polyp and turbinate cases as regards gland stain intensity. Conclusion Serotonin receptors are highly expressed in patients with chronic rhinosinusitis with nasal polyposis in both turbinate and polypoidal tissues which proves that serotonin has a strong role in the formation and growth of nasal polypi and allergic reactions. Hence serotonin modulating drugs can be studied as a new therapy for chronic rhinosinusitis with nasal polyposis.
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慢性鼻窦炎伴鼻息肉患者和正常鼻黏膜中血清素能(5HT)受体的表达
摘要 一项病例对照研究:慢性鼻炎伴鼻息肉患者和正常鼻黏膜中 5HT 受体的表达。背景 慢性鼻炎伴鼻息肉病是治疗和管理方面具有挑战性的疾病之一。最近的许多研究引入了新的治疗方法,如靶向免疫调节药物而非抗组胺药物、局部和全身性类固醇以及鼻窦内窥镜手术。通过研究其免疫学途径和参与炎症过程的物质来了解疾病背后的病理,有助于引入新的治疗方案,从而改善治疗效果并降低治疗成本。目的 我们的研究旨在证明慢性鼻息肉伴鼻息肉症患者的鼻息肉和下鼻甲粘膜中存在 5-HT 受体,然后将这种 5-HT 受体的表达与正常对照组的下鼻甲粘膜进行比较。方法 对 80 名患者进行了病例对照研究。慢性鼻息肉患者(病例)的鼻息肉和鼻甲粘膜标本与正常鼻甲粘膜标本(对照组)在艾因夏姆斯大学医院病理科实验室用自动免疫染色机进行血清素受体免疫组化染色,染色比例为生产公司推荐的 1:200-400。然后,比较粘膜、腺体和血管的染色分布和强度。结果 息肉病例与对照组在粘膜上皮和粘膜腺体染色分布和染色强度方面存在明显差异。在血管应变分布方面,息肉病例与对照组也存在明显差异。但是,在血管染色强度方面没有发现明显差异。鼻甲病例与对照组在衬里上皮染色分布和染色强度方面有明显差异。鼻甲病例与对照组在腺体染色分布方面没有明显差异。但是,鼻甲和对照组在腺体染色强度方面有明显差异。就血管染色分布而言,鼻甲病例与对照组之间存在显著差异;但就染色强度而言,两者之间没有显著差异。息肉病例和鼻甲病例在衬里上皮和血管染色分布及染色强度方面没有明显差异。息肉和鼻甲在腺体染色分布方面无明显差异,但在腺体染色强度方面差异很大。结论 羟色胺受体在慢性鼻炎伴鼻息肉患者的鼻甲和息肉组织中均高度表达,这证明羟色胺在鼻息肉的形成和生长以及过敏反应中起着重要作用。因此,血清素调节药物可作为慢性鼻炎伴鼻息肉病的一种新疗法进行研究。
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