Biological Function of Keratin-Associated Protein 26.1 of Liaoning Cashmere Goat Gene Family

Pub Date : 2024-04-01 DOI:10.3103/s0095452724020087

Mei Jin, Youyue Wang, Hongbo Zhang, Xinyue Qiu

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Abstract

We obtained the full-length cDNA clone of KAP26.1 in Liaoning cashmere goat, and then we further investigated biological functions of KAP26.1. First, we discovered KAP26.1 was specifically expressed in internal root sheath of skin hair follicles by semi-quantitative reverse transcription and polymerase chain reaction (semi-quantitative RT-PCR), hybridization in situ; immunohistochemistry revealed KAP26.1 was located in the internal and external root sheaths. Next, quantitative real-time polymerase chain reaction (qRT-PCR) results showed relative KAP26.1 expression quantity was significantly different between primary and secondary follicles during anagen, catagen, and remarkably increased during telogen. Moreover, after inhibiting Noggin expression, we found relative KAP26.1 expression quantity significantly declined; after KAP26.1 overexpression, we found relative Noggin expression quantity highly significantly declined. Finally, we found MT played a positive role in KAP26.1 and KAP26.1 expression; FGF5 and IGF-1 palyed a negative role in KAP26.1 and blocked the degradation of KAP26.1. The results revealed KAP26.1 played an important role in regulating fine hair development.

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辽宁绒山羊基因家族角蛋白相关蛋白 26.1 的生物学功能

我们从辽宁绒山羊中获得了KAP26.1的全长cDNA克隆，并进一步研究了KAP26.1的生物学功能。首先，我们通过半定量反转录聚合酶链式反应（semi-quantitative reverse transcription and polymerase chain reaction，RT-PCR）和原位杂交发现KAP26.1在皮肤毛囊的内根鞘中特异表达；免疫组化显示KAP26.1位于内外根鞘中。接着，实时定量聚合酶链反应（qRT-PCR）结果显示，KAP26.1的相对表达量在原发和继发滤泡的生长期和延缓期有显著差异，在休止期则明显增加。此外，在抑制Noggin表达后，我们发现KAP26.1的相对表达量显著下降；在KAP26.1过表达后，我们发现Noggin的相对表达量高度显著下降。最后，我们发现MT对KAP26.1和KAP26.1的表达起正向作用；FGF5和IGF-1对KAP26.1起负向作用，并阻止KAP26.1的降解。研究结果表明，KAP26.1在调节细毛发育中发挥着重要作用。

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