Characterization of the Complete Chloroplast Genome and Evolutionary Position of Clematis tomentella

Pub Date : 2024-04-01 DOI:10.3103/s0095452724020075

Wangsuo Liu, Zhanjun Wang, Ying Tian, Bo Ji

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Abstract

Clematis tomentella 2001 (Ranunculaceae) is a typical drought-tolerant sand-fixing plant in desert ecosystem in northwest China. To elucidate the phylogenetic status of C. tomentella and its related species, we determined the complete chloroplast (cp) genome and analyzed their interspecific relationships. The result showed that the cp genome of C. tomentella was 159 816 bp in length, including two inverted repeats of 31 045 bp, a large single copy region of 79 535 bp, and a small single copy region of 18 191 bp. 136 genes were annotated across the whole cp genome, including 92 protein-coding genes, 8 rRNA genes, and 36 tRNA genes, as well as the GC content accounted for 38%. Crucially, we found that the regions of psbE-petL, trnG_UCC-atpA, ndhF-rpl32, and rps8-infA were highly divergent, which could be marked as DNA barcodes for the identification of C. tomentella in Ranunculaceae. A maximum likelihood phylogenetic tree revealed that C. tomentella was closely related to C. fruticosa. Our results provide the references and implications for the phylogenetic study of Clematis in Ranunculaceae in the future.

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Clematis tomentella 完整叶绿体基因组的特征和进化定位

铁线莲（Clematis tomentella 2001）（毛茛科）是中国西北荒漠生态系统中典型的耐旱固沙植物。为阐明铁线莲及其近缘种的系统发育状况，我们测定了铁线莲的完整叶绿体（cp）基因组，并分析了它们的种间关系。结果表明，C. tomentella 的 cp 基因组全长 159 816 bp，包括两个 31 045 bp 的倒位重复区、一个 79 535 bp 的大单个拷贝区和一个 18 191 bp 的小单个拷贝区。整个 cp 基因组共注释了 136 个基因，包括 92 个蛋白质编码基因、8 个 rRNA 基因和 36 个 tRNA 基因，GC 含量占 38%。最重要的是，我们发现psbE-petL、trnG_UCC-atpA、ndhF-rpl32和rps8-infA的区域差异很大，可作为鉴定毛茛科C.最大似然法系统发生树显示，C. tomentella与C. fruticosa亲缘关系密切。我们的研究结果为今后对毛茛科铁线莲属植物的系统发育研究提供了参考和启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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