Acoustofluidic Microbioreactor Using Surface Acoustic Wave-induced Acoustic Streaming Flow

IF 5.5 3区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS BioChip Journal Pub Date : 2024-04-02 DOI:10.1007/s13206-024-00148-0

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Abstract

Microbioreactors have been widely utilized as an alternative to conventional benchtop reactors, since the miniaturized platforms offer advantages including reduced sample volume and homogeneous microenvironments. Here, we proposed an acoustofluidic microbioreactor based on surface acoustic wave (SAW)-induced acoustic streaming flow (ASF). The SAW-induced ASF, which originates from the wave attenuation in a fluid, allows rapid mixing and heat transfer for enhanced mass and heat transfer within the sample fluid. We conducted thorough numerical and experimental investigations on the acousto-hydrodynamics and heat transfer phenomena to find an optimal frequency in the prescribed cylindrical microwell. We found that the homogenous chemical concentration and temperature distributions within the fluid were rapidly achieved by the SAW-induced ASF in the proposed device. For proof-of-concept demonstration of practical applicability, we cultured Escherichia coli as a model cell using the proposed acoustofluidic microbioreactor. From comparative evaluation with conventional platforms including a shaker incubator and a microplate shaker, we confirmed that the bacteria growth rate was enhanced in the proposed acoustofluidic microbioreactor due to the high homogeneity in the chemical concentration and temperature by the acoustic agitation, without any moving mechanical components. We expect that the proposed ASF-based microbioreactor can be broadly utilized for various biological applications that require homogeneous mixing and temperature gradient within a reaction medium.

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利用表面声波诱导声流的声流体微生物反应器

摘要微型生物反应器已被广泛用作传统台式反应器的替代品，因为微型平台具有减少样品体积和均质微环境等优势。在此，我们提出了一种基于表面声波（SAW）诱导的声学流动（ASF）的声学流体微生物反应器。声表面波诱导的 ASF 源于波在流体中的衰减，可实现快速混合和传热，从而增强样品流体内的传质和传热。我们对声-流体力学和传热现象进行了深入的数值和实验研究，以找到规定的圆柱形微孔中的最佳频率。我们发现，在所提出的装置中，声表面波诱导的 ASF 能够快速实现流体中均匀的化学浓度和温度分布。为了对实际应用性进行概念验证，我们使用所提出的声学流体微生物反应器培养大肠杆菌作为模型细胞。通过与传统平台（包括振动培养箱和微孔板振动器）的比较评估，我们证实，在拟议的声学流体微生物反应器中，由于声学搅拌使化学浓度和温度高度均匀，细菌的生长率得到了提高，而且没有任何移动的机械部件。我们希望所提出的基于 ASF 的微型生物反应器能广泛应用于需要在反应介质中实现均匀混合和温度梯度的各种生物应用领域。

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来源期刊

BioChip Journal 生物-生化研究方法

CiteScore

7.70

自引率

16.30%

发文量

审稿时长

6-12 weeks

期刊介绍： BioChip Journal publishes original research and reviews in all areas of the biochip technology in the following disciplines, including protein chip, DNA chip, cell chip, lab-on-a-chip, bio-MEMS, biosensor, micro/nano mechanics, microfluidics, high-throughput screening technology, medical science, genomics, proteomics, bioinformatics, medical diagnostics, environmental monitoring and micro/nanotechnology. The Journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.