Transmission of Xylella fastidiosa subspecies multiplex from naturally infected to healthy Rhamnus alaternus by Philaenus spumarius and Neophilaenus campestris

IF 4.3 1区 农林科学 Q1 ENTOMOLOGY Journal of Pest Science Pub Date : 2024-04-16 DOI:10.1007/s10340-024-01775-0
Anita Nencioni, Elisabetta Gargani, Agostino Strangi, Domenico Rizzo, Immacolata Iovinella, Patrizia Sacchetti, Pio Federico Roversi, Ilaria Cutino
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Abstract

In Europe, the recently reported plant pathogen Xylella fastidiosa subsp. multiplex affects several wild, ornamental, and cultivated trees causing scorch diseases. In 2018, the sequence type 87 was reported in Tuscany on Mediterranean shrubs and trees. Although spittlebugs (Hemiptera: Aphrophoridae) were already identified as main vectors of this bacterium in Europe, their role in the transmission of this subspecies has not been ascertained yet. In this study the ability of Philaenus spumarius and Neophilaenus campestris to acquire and transmit Xylella fastidiosa subsp. multiplex sequence type 87 from and to Rhamnus alaternus was evaluated in two-year semi-field experiments. To acquire the bacterium, insects were confined on wild, naturally infected R. alaternus shrubs for 120 h. Then, they were transferred to healthy plants and maintained in cages for 96 h. To follow the infection, plant samples were collected every two months for three times. Tested plants were destroyed at the end of experiments and roots, twigs and leaves were analysed. Philaenus spumarius showed a significantly higher survival rate than N. campestris. The infection status of both insects and plants was assessed through molecular analysis. P. spumarius and N. campestris were able to infect healthy plants although the acquisition rate and the estimated probability of transmission appeared to be low. These findings provide new accounts on the role of two polyphagous insect vectors in spreading a quarantine organism, which is lethal to a huge number of plant species. However, further studies are needed to disclose more specific interactions within this complex pathosystem.

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通过Phaenus spumarius和Neophilaenus campestris将Xylella fastidiosa亚种多重菌从自然感染的鼠李树传播到健康的鼠李树
在欧洲,最近报道的植物病原体 Xylella fastidiosa subsp.multiplex影响着多种野生、观赏和栽培树木,导致焦枯病。2018 年,在托斯卡纳报告了地中海灌木和树木上的序列 87 型。虽然唾蝇(半翅目:蚜蝇科)已被确定为该细菌在欧洲的主要传播媒介,但它们在该亚种传播中的作用尚未确定。本研究在为期两年的半田间试验中评估了 Philaenus spumarius 和 Neophilaenus campestris 从鼠尾草中获取并向鼠尾草传播 Xylella fastidiosa 亚种多重序列 87 型的能力。为了获得该细菌,昆虫被关在野生、自然感染的鼠李灌木上 120 小时,然后被转移到健康植物上,并在笼子里保持 96 小时。实验结束后销毁受试植物,并对根、枝和叶进行分析。从实验结果来看,Phaenus spumarius 的存活率明显高于 N. campestris。通过分子分析评估了昆虫和植物的感染状况。尽管感染率和估计的传播概率似乎都很低,但 P. spumarius 和 N. campestris 仍能感染健康植物。这些发现为两种多食性昆虫载体在传播检疫生物方面的作用提供了新的说明,这种检疫生物对大量植物物种都是致命的。然而,要揭示这一复杂病理生态系统中更具体的相互作用,还需要进一步的研究。
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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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