Eleonora Bailoni, Miyer F. Patiño-Ruiz, Andreea R. Stan, Gea K. Schuurman-Wolters, Marten Exterkate, Arnold J. M. Driessen and Bert Poolman*,
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引用次数: 0
Abstract
ATP is a universal energy currency that is essential for life. l-Arginine degradation via deamination is an elegant way to generate ATP in synthetic cells, which is currently limited by a slow l-arginine/l-ornithine exchange. We are now implementing a new antiporter with better kinetics to obtain faster ATP recycling. We use l-arginine-dependent ATP formation for the continuous synthesis and export of glycerol 3-phosphate by including glycerol kinase and the glycerol 3-phosphate/Pi antiporter. Exported glycerol 3-phosphate serves as a precursor for the biosynthesis of phospholipids in a second set of vesicles, which forms the basis for the expansion of the cell membrane. We have therefore developed an out-of-equilibrium metabolic network for ATP recycling, which has been coupled to lipid synthesis. This feeder–utilizer system serves as a proof-of-principle for the systematic buildup of synthetic cells, but the vesicles can also be used to study the individual reaction networks in confinement.
通过脱氨基作用降解精氨酸是在合成细胞中生成 ATP 的一种有效方法,但目前这种方法受限于缓慢的精氨酸/鸟氨酸交换。目前,我们正在采用一种动力学性能更好的新型反转运体,以获得更快的 ATP 循环。我们利用依赖于精氨酸的 ATP 形成,通过甘油激酶和甘油-3-磷酸/Pi 反转运体,持续合成和输出甘油-3-磷酸。输出的甘油-3-磷酸酯是第二组囊泡中磷脂生物合成的前体,是细胞膜扩张的基础。因此,我们开发了一个用于 ATP 循环的失衡代谢网络,并将其与脂质合成结合起来。这种馈源-利用系统可作为系统建立合成细胞的原理验证,但小泡也可用于研究封闭的单个反应网络。
期刊介绍:
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.