Pub Date : 2024-11-19DOI: 10.1021/acssynbio.4c00388
Fereshteh Jafarbeglou, Mary J Dunlop
Optogenetic tools have been used in a wide range of microbial engineering applications that benefit from the tunable, spatiotemporal control that light affords. However, the majority of current optogenetic constructs for bacteria respond to blue light, limiting the potential for multichromatic control. In addition, other wavelengths offer potential benefits over blue light, including improved penetration of dense cultures and reduced potential for toxicity. In this study, we introduce OptoCre-REDMAP, a red light inducible Cre recombinase system in Escherichia coli. This system harnesses the plant photoreceptors PhyA and FHY1 and a split version of Cre recombinase to achieve precise control over gene expression and DNA excision. We optimized the design by modifying the start codon of Cre and characterized the impact of different levels of induction to find conditions that produced minimal basal expression in the dark and induced full activation within 4 h of red light exposure. We characterized the system's sensitivity to ambient light, red light intensity, and exposure time, finding OptoCre-REDMAP to be reliable and flexible across a range of conditions. In coculture experiments with OptoCre-REDMAP and the blue light responsive OptoCre-VVD, we found that the systems responded orthogonally to red and blue light inputs. Direct comparisons between red and blue light induction with OptoCre-REDMAP and OptoCre-VVD demonstrated the superior penetration properties of red light. OptoCre-REDMAP's robust and selective response to red light makes it suitable for advanced synthetic biology applications, particularly those requiring precise multichromatic control.
{"title":"Red Light Responsive Cre Recombinase for Bacterial Optogenetics.","authors":"Fereshteh Jafarbeglou, Mary J Dunlop","doi":"10.1021/acssynbio.4c00388","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00388","url":null,"abstract":"<p><p>Optogenetic tools have been used in a wide range of microbial engineering applications that benefit from the tunable, spatiotemporal control that light affords. However, the majority of current optogenetic constructs for bacteria respond to blue light, limiting the potential for multichromatic control. In addition, other wavelengths offer potential benefits over blue light, including improved penetration of dense cultures and reduced potential for toxicity. In this study, we introduce OptoCre-REDMAP, a red light inducible Cre recombinase system in <i>Escherichia coli</i>. This system harnesses the plant photoreceptors PhyA and FHY1 and a split version of Cre recombinase to achieve precise control over gene expression and DNA excision. We optimized the design by modifying the start codon of Cre and characterized the impact of different levels of induction to find conditions that produced minimal basal expression in the dark and induced full activation within 4 h of red light exposure. We characterized the system's sensitivity to ambient light, red light intensity, and exposure time, finding OptoCre-REDMAP to be reliable and flexible across a range of conditions. In coculture experiments with OptoCre-REDMAP and the blue light responsive OptoCre-VVD, we found that the systems responded orthogonally to red and blue light inputs. Direct comparisons between red and blue light induction with OptoCre-REDMAP and OptoCre-VVD demonstrated the superior penetration properties of red light. OptoCre-REDMAP's robust and selective response to red light makes it suitable for advanced synthetic biology applications, particularly those requiring precise multichromatic control.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142666406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-18DOI: 10.1021/acssynbio.4c00149
Sofija Semeniuk, Bin-Zhi Qian, Elise Cachat
Immune cells play a pivotal role in the establishment, growth, and progression of tumors at primary and metastatic sites. Macrophages, in particular, play a critical role in suppressing immune responses and promoting an anti-inflammatory environment through both direct and indirect cell-cell interactions. However, our understanding of the mechanisms underlying such interactions is limited due to a lack of reliable tools for studying transient interactions between cancer cells and macrophages within the tumor microenvironment. Recent advances in mammalian synthetic biology have introduced a wide range of synthetic receptors that have been used in diverse biosensing applications. One such synthetic receptor is the synNotch receptor, which can be tailored to sense specific ligands displayed on the surface of target cells. With this study, we aimed at developing a novel αCD206-synNotch receptor, targeting CD206+ macrophages, a population of macrophages that play a crucial role in promoting metastatic seeding and persistent growth. Engineered in cancer cells and used in mouse metastasis models, such a tool could help monitor─and provide an understanding of─the effects that cell-cell interactions between macrophages and cancer cells have on metastasis establishment. Here, we report the development of cancer landing-pad cells for versatile applications and the engineering of αCD206-synNotch cancer cells in particular. We report the measurement of their activity and specificity, and discuss unexpected caveats regarding their in vivo applications.
{"title":"Engineering an αCD206-synNotch Receptor: Insights into the Development of Novel Synthetic Receptors.","authors":"Sofija Semeniuk, Bin-Zhi Qian, Elise Cachat","doi":"10.1021/acssynbio.4c00149","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00149","url":null,"abstract":"<p><p>Immune cells play a pivotal role in the establishment, growth, and progression of tumors at primary and metastatic sites. Macrophages, in particular, play a critical role in suppressing immune responses and promoting an anti-inflammatory environment through both direct and indirect cell-cell interactions. However, our understanding of the mechanisms underlying such interactions is limited due to a lack of reliable tools for studying transient interactions between cancer cells and macrophages within the tumor microenvironment. Recent advances in mammalian synthetic biology have introduced a wide range of synthetic receptors that have been used in diverse biosensing applications. One such synthetic receptor is the synNotch receptor, which can be tailored to sense specific ligands displayed on the surface of target cells. With this study, we aimed at developing a novel αCD206-synNotch receptor, targeting CD206<sup>+</sup> macrophages, a population of macrophages that play a crucial role in promoting metastatic seeding and persistent growth. Engineered in cancer cells and used in mouse metastasis models, such a tool could help monitor─and provide an understanding of─the effects that cell-cell interactions between macrophages and cancer cells have on metastasis establishment. Here, we report the development of cancer landing-pad cells for versatile applications and the engineering of αCD206-synNotch cancer cells in particular. We report the measurement of their activity and specificity, and discuss unexpected caveats regarding their <i>in vivo</i> applications.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial electrochemical systems (MESs), as a green and sustainable technology, can decompose organics in wastewater to recover bioelectricity. Electroactive biofilms, a microbial community structure encased in a self-produced matrix, play a decisive role in determining the efficiency of MESs. However, as an essential component of the biofilm matrix, the role of exopolysaccharides in electroactive biofilm formation and their influence on extracellular electron transfer (EET) have been rarely studied. Herein, to explore the effects of exopolysaccharides on biofilm formation and EET rate, we first inhibited the key genes responsible for exopolysaccharide biosynthesis (namely, so_3171, so_3172, so_3177, and so_3178) by using antisense RNA in Shewanella oneidensis MR-1. Then, to explore the underlying mechanisms why inhibition of exopolysaccharide synthesis could enhance biofilm formation and promote the EET rate, we characterized cell physiology and electrophysiology. The results showed inhibition of exopolysaccharide biosynthesis not only altered cell surface hydrophobicity and promoted intercellular adhesion and aggregation, but also increased biosynthesis of c-type cytochromes and decreased interfacial resistance, thus promoting electroactive biofilm formation and improving the EET rate of S. oneidensis. Lastly, to evaluate and intensify the capability of exopolysaccharide-reduced strains in harvesting electrical energy from actual liquor wastewater, engineered strain Δ3171-as3177 was further constructed to treat an actual thin stillage. The results showed that the output power density reached 380.98 mW m-2, 11.1-fold higher than that of WT strain, which exhibited excellent capability of harvesting electricity from actual liquor wastewater. This study sheds light on the underlying mechanism of how inhibition of exopolysaccharides impacts electroactive biofilm formation and EET rate, which suggested that regulating exopolysaccharide biosynthesis is a promising avenue for increasing the EET rate.
{"title":"Engineering Exopolysaccharide Biosynthesis of <i>Shewanella oneidensis</i> to Promote Electroactive Biofilm Formation for Liquor Wastewater Treatment.","authors":"Zixuan You, Huan Yu, Baocai Zhang, Qijing Liu, Bo Xiong, Chao Li, Chunxiao Qiao, Longhai Dai, Jianxun Li, Wenwei Li, Guosheng Xin, Zhanying Liu, Feng Li, Hao Song","doi":"10.1021/acssynbio.4c00417","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00417","url":null,"abstract":"<p><p>Microbial electrochemical systems (MESs), as a green and sustainable technology, can decompose organics in wastewater to recover bioelectricity. Electroactive biofilms, a microbial community structure encased in a self-produced matrix, play a decisive role in determining the efficiency of MESs. However, as an essential component of the biofilm matrix, the role of exopolysaccharides in electroactive biofilm formation and their influence on extracellular electron transfer (EET) have been rarely studied. Herein, to explore the effects of exopolysaccharides on biofilm formation and EET rate, we first inhibited the key genes responsible for exopolysaccharide biosynthesis (namely, <i>so_3171</i>, <i>so_3172</i>, <i>so_3177</i>, and <i>so_3178</i>) by using antisense RNA in <i>Shewanella oneidensis</i> MR-1. Then, to explore the underlying mechanisms why inhibition of exopolysaccharide synthesis could enhance biofilm formation and promote the EET rate, we characterized cell physiology and electrophysiology. The results showed inhibition of exopolysaccharide biosynthesis not only altered cell surface hydrophobicity and promoted intercellular adhesion and aggregation, but also increased biosynthesis of <i>c</i>-type cytochromes and decreased interfacial resistance, thus promoting electroactive biofilm formation and improving the EET rate of <i>S. oneidensis</i>. Lastly, to evaluate and intensify the capability of exopolysaccharide-reduced strains in harvesting electrical energy from actual liquor wastewater, engineered strain Δ3171-as3177 was further constructed to treat an actual thin stillage. The results showed that the output power density reached 380.98 mW m<sup>-2</sup>, 11.1-fold higher than that of WT strain, which exhibited excellent capability of harvesting electricity from actual liquor wastewater. This study sheds light on the underlying mechanism of how inhibition of exopolysaccharides impacts electroactive biofilm formation and EET rate, which suggested that regulating exopolysaccharide biosynthesis is a promising avenue for increasing the EET rate.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Intrahepatic cholestasis of pregnancy (ICP) is the most common and high-risk liver disorder during pregnancy, with varying prevalence across populations. Our understanding of the mechanisms underlying ICP and its population difference remains limited. This study delves into the genetic etiology of ICP in East Asians, drawing comparisons with Europeans to comprehend ICP etiology in the context of genetic background and evolution.
Methods
We conducted the hitherto largest-scale genome-wide association studies (GWAS) on fasting total serum bile acids (TBA) and ICP in 98,269 Chinese pregnancies. The findings were replicated in three cohorts and compared with European populations. Additionally, phenome-wide association and spatio-temporal evolution analyses were employed to investigate the function and evolutionary patterns of ICP-associated loci.
Results
We identified eight loci for fasting TBA and four for ICP, including ten novel loci. Notably, we discovered an East-Asian-specific locus within a 0.4Mbp region at 14q24.1, which increases fasting TBA by 6.12 μmol/L and ICP risk by 16.56-fold per risk allele (95% CI: 16.43 to 16.69, P = 7.06×10-381). Phenome-wide association and spatial-temporal evolution analyses revealed that this 14q24.1 ICP risk locus confers resistance to hepatitis B and has become prevalent in East and Southeast Asia within the last 3,000 years.
Conclusions
We uncovered a distinct genetic etiology of ICP in East Asians, likely linked to a historic HBV epidemic in East and Southeast Asia within the last 3,000 years. These findings enhance our understanding of ICP pathophysiology and offer potential for more precise detection, assessment, and treatment of the disorder.
Impact and implications
This study provides novel insights into the genetic basis of intrahepatic cholestasis of pregnancy (ICP) in East Asian populations, where little was previously known. The identification of the East-Asian-specific 14q24.1 locus, associated with both fasting TBA and ICP, and its connection to a historical hepatitis B epidemic emphasize the importance of incorporating population-specific history into disease research. These findings are crucial for researchers studying pregnancy-related liver disorders and clinicians providing care to pregnant women, enabling more accurate screening, risk assessment, and targeted interventions for ICP.
{"title":"Genetic study of intrahepatic cholestasis of pregnancy in Chinese women unveils East Asian etiology linked to historic HBV epidemic","authors":"Yanhong Liu, Yuandan Wei, Xiaohang Chen, Shujia Huang, Yuqin Gu, Zijing Yang, Xinxin Guo, Hao Zheng, Hanxiao Feng, Mingxi Huang, Shangliang Chen, Tiantian Xiao, Liang Hu, Quanfu Zhang, Yang Zhang, Guo-Bo Chen, Xiu Qiu, Fengxiang Wei, Jianxin Zhen, Siyang Liu","doi":"10.1016/j.jhep.2024.11.008","DOIUrl":"https://doi.org/10.1016/j.jhep.2024.11.008","url":null,"abstract":"<h3>Background & Aims</h3>Intrahepatic cholestasis of pregnancy (ICP) is the most common and high-risk liver disorder during pregnancy, with varying prevalence across populations. Our understanding of the mechanisms underlying ICP and its population difference remains limited. This study delves into the genetic etiology of ICP in East Asians, drawing comparisons with Europeans to comprehend ICP etiology in the context of genetic background and evolution.<h3>Methods</h3>We conducted the hitherto largest-scale genome-wide association studies (GWAS) on fasting total serum bile acids (TBA) and ICP in 98,269 Chinese pregnancies. The findings were replicated in three cohorts and compared with European populations. Additionally, phenome-wide association and spatio-temporal evolution analyses were employed to investigate the function and evolutionary patterns of ICP-associated loci.<h3>Results</h3>We identified eight loci for fasting TBA and four for ICP, including ten novel loci. Notably, we discovered an East-Asian-specific locus within a 0.4Mbp region at 14q24.1, which increases fasting TBA by 6.12 μmol/L and ICP risk by 16.56-fold per risk allele (95% <em>CI</em>: 16.43 to 16.69, <em>P</em> = 7.06×10<sup>-381</sup>). Phenome-wide association and spatial-temporal evolution analyses revealed that this 14q24.1 ICP risk locus confers resistance to hepatitis B and has become prevalent in East and Southeast Asia within the last 3,000 years.<h3>Conclusions</h3>We uncovered a distinct genetic etiology of ICP in East Asians, likely linked to a historic HBV epidemic in East and Southeast Asia within the last 3,000 years. These findings enhance our understanding of ICP pathophysiology and offer potential for more precise detection, assessment, and treatment of the disorder.<h3>Impact and implications</h3>This study provides novel insights into the genetic basis of intrahepatic cholestasis of pregnancy (ICP) in East Asian populations, where little was previously known. The identification of the East-Asian-specific 14q24.1 locus, associated with both fasting TBA and ICP, and its connection to a historical hepatitis B epidemic emphasize the importance of incorporating population-specific history into disease research. These findings are crucial for researchers studying pregnancy-related liver disorders and clinicians providing care to pregnant women, enabling more accurate screening, risk assessment, and targeted interventions for ICP.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"36 1","pages":""},"PeriodicalIF":25.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Membranes with advanced and novel functions play important roles in emerging applications ranging from industrial separations, water purification, energy harvesting and storage, health care, biomimetic membranes and more. The performances of membranes in these critical applications are essentially determined by their interfacial interactions with surrounding ions, molecules, particles, emulsions, and bioactive agents. Amphiphilic copolymers containing both hydrophobic and hydrophilic segments will spontaneously assemble into multiphase and hierarchical structures, providing a general solution for regulating the surface physical-chemical properties of membranes used in the aforementioned urgent applications. Controlled synthesis of amphiphilic copolymers and the methods to fabricate copolymers into membranes with predetermined performance are fundamentally important for their applications. In this work, we first summarize the polymerization techniques developed to synthesize amphiphilic copolymers used for membrane materials. We then review the methods to fabricate membranes from amphiphilic copolymers, and summarize urgent applications of advanced functional membranes produced from amphiphilic copolymers. We also discuss some remaining challenges and provide remarks for future perspectives, especially considering that the circular polymer economy and artificial intelligence have already set new requirements for polymer science. This work offers a comprehensive overview of recent advances in functional materials based on amphiphilic polymers, including the working principles and associations between polymer structure, processing strategy, and membrane performances, which can provide new insights into the development of high performance and next generation of polymeric membranes through the precise, function-led synthesis of novel amphiphilic copolymers and controlled membrane fabrication process.
{"title":"Advanced Functional Membranes Based on Amphiphilic Copolymers","authors":"Zhuan Yi, Lijing Zhu, Ruiyan Xiong, Chuanjie Fang, Baoku Zhu, Liping Zhu, Hongbo Zeng","doi":"10.1016/j.progpolymsci.2024.101907","DOIUrl":"https://doi.org/10.1016/j.progpolymsci.2024.101907","url":null,"abstract":"Membranes with advanced and novel functions play important roles in emerging applications ranging from industrial separations, water purification, energy harvesting and storage, health care, biomimetic membranes and more. The performances of membranes in these critical applications are essentially determined by their interfacial interactions with surrounding ions, molecules, particles, emulsions, and bioactive agents. Amphiphilic copolymers containing both hydrophobic and hydrophilic segments will spontaneously assemble into multiphase and hierarchical structures, providing a general solution for regulating the surface physical-chemical properties of membranes used in the aforementioned urgent applications. Controlled synthesis of amphiphilic copolymers and the methods to fabricate copolymers into membranes with predetermined performance are fundamentally important for their applications. In this work, we first summarize the polymerization techniques developed to synthesize amphiphilic copolymers used for membrane materials. We then review the methods to fabricate membranes from amphiphilic copolymers, and summarize urgent applications of advanced functional membranes produced from amphiphilic copolymers. We also discuss some remaining challenges and provide remarks for future perspectives, especially considering that the circular polymer economy and artificial intelligence have already set new requirements for polymer science. This work offers a comprehensive overview of recent advances in functional materials based on amphiphilic polymers, including the working principles and associations between polymer structure, processing strategy, and membrane performances, which can provide new insights into the development of high performance and next generation of polymeric membranes through the precise, function-led synthesis of novel amphiphilic copolymers and controlled membrane fabrication process.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"40 1","pages":""},"PeriodicalIF":27.1,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.jhep.2024.10.046
Frank J. Gonzalez, Yangliu Xia
Section snippets
Financial support
Funded by the National Cancer Institute Intramural Research Program.
Declaration of Competing Interest
The authors declare no conflicts of interest that pertain to this work.Please refer to the accompanying ICMJE disclosure forms for further details.Adipose triglyceride lipase (ATGL), encoded by the PNPLA2 gene in humans, hydrolyzes triacylglycerols to diacylglycerols. PNPLA2 is regulated by insulin, and under conditions of insulin resistance, increased adipocyte ATGL leads to excess production of free fatty acids that are transported to the liver resulting in metabolic dysfunction-associated
{"title":"Adipose triglyceride lipase as a target for treatment of metabolic dysfunction-associated steatohepatitis: the role of hepatic and intestinal PPARα","authors":"Frank J. Gonzalez, Yangliu Xia","doi":"10.1016/j.jhep.2024.10.046","DOIUrl":"https://doi.org/10.1016/j.jhep.2024.10.046","url":null,"abstract":"<h2>Section snippets</h2><section><section><h2>Financial support</h2>Funded by the National Cancer Institute Intramural Research Program.</section></section><section><section><h2>Declaration of Competing Interest</h2>The authors declare no conflicts of interest that pertain to this work.Please refer to the accompanying ICMJE disclosure forms for further details.Adipose triglyceride lipase (ATGL), encoded by the <em>PNPLA2</em> gene in humans, hydrolyzes triacylglycerols to diacylglycerols. <em>PNPLA2</em> is regulated by insulin, and under conditions of insulin resistance, increased adipocyte ATGL leads to excess production of free fatty acids that are transported to the liver resulting in metabolic dysfunction-associated</section></section>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"37 1","pages":""},"PeriodicalIF":25.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Activating hepatobiliary water channels for gallstone prevention in complicated gallstone disease","authors":"Jan G. Hengstler, Nachiket Vartak","doi":"10.1016/j.jhep.2024.11.006","DOIUrl":"https://doi.org/10.1016/j.jhep.2024.11.006","url":null,"abstract":"<h2>Section snippets</h2><section><section><h2>Conflict of interest statement</h2>The authors declare no conflict of interest.</section></section><section><section><h2>Uncited reference</h2>[17]; [18]; [19]; [17]; [18]; [19].</section></section><section><section><h2>Authors' contributions</h2>JGH and NV developed the ideas presented and wrote the manuscript.</section></section><section><section><h2>Financial support</h2>No particulars to report</section></section>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"35 1","pages":""},"PeriodicalIF":25.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.jhep.2024.11.011
Emma Vanderschueren
Section snippets
Author contributions
Conceptualization, data curation, formal analysis and writing of the manuscript were performed by EV and WL.
Financial support
None.
Declaration of Competing Interest
WL serves as a consultant for Cook Medical, MRM Health, and CSL Behring, and has received speaker’s fees from Boston Scientific. No other conflicts of interest were withheld.
Acknowledgements
We wish to thank Prof. Antonio Colecchia for his assistance in providing additional data from the validation cohort.
作者贡献构思、数据整理、正式分析和手稿撰写由 EV 和 WL 完成。财务支持无。竞争利益声明WL 担任 Cook Medical、MRM Health 和 CSL Behring 的顾问，并从波士顿科学公司获得演讲费。没有隐瞒其他利益冲突。感谢 Antonio Colecchia 教授协助提供验证队列的其他数据。
{"title":"Non-invasive algorithms could outperform HVPG in selecting candidates for non-selective beta-blockers in cirrhosis","authors":"Emma Vanderschueren","doi":"10.1016/j.jhep.2024.11.011","DOIUrl":"https://doi.org/10.1016/j.jhep.2024.11.011","url":null,"abstract":"<h2>Section snippets</h2><section><section><h2>Author contributions</h2>Conceptualization, data curation, formal analysis and writing of the manuscript were performed by EV and WL.</section></section><section><section><h2>Financial support</h2>None.</section></section><section><section><h2>Declaration of Competing Interest</h2>WL serves as a consultant for Cook Medical, MRM Health, and CSL Behring, and has received speaker’s fees from Boston Scientific. No other conflicts of interest were withheld.</section></section><section><section><h2>Acknowledgements</h2>We wish to thank Prof. Antonio Colecchia for his assistance in providing additional data from the validation cohort.</section></section>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"5 1","pages":""},"PeriodicalIF":25.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.jhep.2024.11.010
Cyriac Abby Philips, Arif Hussain Theruvath, Aryalakshmi Sreemohan, John Menachery, Rosh Varghese
Section snippets
Authors’ contributions
CAP designed the study and wrote the manuscript, AHT and AL acquired, compiled, and analysed the data, JM and RV finalized the manuscript and made critical revisions, all authors accepted the final version of manuscript for submission.
Financial support
The authors received no financial support to produce this manuscript.
Declaration of Competing Interest
The authors declare no conflicts of interest that pertain to this work.
{"title":"Surrender to evidence: the futility of plasma exchange for severe liver disease and liver failure","authors":"Cyriac Abby Philips, Arif Hussain Theruvath, Aryalakshmi Sreemohan, John Menachery, Rosh Varghese","doi":"10.1016/j.jhep.2024.11.010","DOIUrl":"https://doi.org/10.1016/j.jhep.2024.11.010","url":null,"abstract":"<h2>Section snippets</h2><section><section><h2>Authors’ contributions</h2>CAP designed the study and wrote the manuscript, AHT and AL acquired, compiled, and analysed the data, JM and RV finalized the manuscript and made critical revisions, all authors accepted the final version of manuscript for submission.</section></section><section><section><h2>Financial support</h2>The authors received no financial support to produce this manuscript.</section></section><section><section><h2>Declaration of Competing Interest</h2>The authors declare no conflicts of interest that pertain to this work.</section></section>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"62 1","pages":""},"PeriodicalIF":25.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This work was supported by the Medical and Health Research Project of Baoan District (No. 2023JD250), and the Key Specialties in Clinical Medicine of the People’s Hospital of Baoan Shenzhen(No. 8).
Declaration of Competing Interest
The authors declare no conflicts of interest that pertain to this work.
{"title":"Unlock AI-Safe-C score's potential at all levels: Improve methods and overcome barriers","authors":"Qin Guo, Hui Li, Chengshan Guo","doi":"10.1016/j.jhep.2024.11.012","DOIUrl":"https://doi.org/10.1016/j.jhep.2024.11.012","url":null,"abstract":"<h2>Section snippets</h2><section><section><h2>Authors contributions</h2>Qin Guo: Conceptualization, Writing – original draft; Hui Li: Conceptualization, Funding acquisition, Writing – review & editing; Chengshan Guo: Funding acquisition, Writing – review & editing.</section></section><section><section><h2>Financial support statement</h2>This work was supported by the Medical and Health Research Project of Baoan District (No. 2023JD250), and the Key Specialties in Clinical Medicine of the People’s Hospital of Baoan Shenzhen(No. 8).</section></section><section><section><h2>Declaration of Competing Interest</h2>The authors declare no conflicts of interest that pertain to this work.</section></section>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"159 1","pages":""},"PeriodicalIF":25.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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