Effects of AI-2 quorum sensing inhibitors on mitigating bacterial contamination in bioethanol production

IF 5.8 2区生物学 Q1 AGRICULTURAL ENGINEERING Biomass & Bioenergy Pub Date : 2024-04-20 DOI:10.1016/j.biombioe.2024.107211

Jun Tian , Yuhai Liang , Arthur J. Ragauskas , Yanming Zhong , Yunqin Lin

{"title":"Effects of AI-2 quorum sensing inhibitors on mitigating bacterial contamination in bioethanol production","authors":"Jun Tian , Yuhai Liang , Arthur J. Ragauskas , Yanming Zhong , Yunqin Lin","doi":"10.1016/j.biombioe.2024.107211","DOIUrl":null,"url":null,"abstract":"<div>In this paper, the activities of signal molecule AI-2 in the sole fermentation of Lactiplantibacillus plantarum and co-fermentation of Saccharomyces cerevisiae and L. plantarum were investigated. Co-fermentation of S. cerevisiae and L. plantarum was found to reduce L. plantarum to secrete AI-2. Three AI-2 quorum sensing inhibitors (DMHF (2,5-Dimethyl-4-hydroxy-3-(2H)-furanone), D-Rib (d-Ribose), and D-Gal (d-Galactosamine)) were added to the co-fermentation system to further interrupt the growth of L. plantarum. It was found that 0.2 g/L DMHF or 18.0 g/L D-Gal could mainly decrease the activity of AI-2 by 38 % and 36 %, respectively, and significantly inhibit the biofilm formation of L. plantarum. Moreover, the ethanol concentrations of the co-fermentation trials added with 0.2 g/L DMHF or 18.0 g/L D-Gal were 14 % and 103 % higher than that of the control, respectively, within 30 h of fermentation. This study provides basic data for the novel prevention of L. plantarum contamination in bioethanol production via quorum sensing control, which is meaningful for the environmental protection and the sustainable development of bioethanol industry.</div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":null,"pages":null},"PeriodicalIF":5.8000,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomass & Bioenergy","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0961953424001648","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}

引用次数: 0

Abstract

In this paper, the activities of signal molecule AI-2 in the sole fermentation of Lactiplantibacillus plantarum and co-fermentation of Saccharomyces cerevisiae and L. plantarum were investigated. Co-fermentation of S. cerevisiae and L. plantarum was found to reduce L. plantarum to secrete AI-2. Three AI-2 quorum sensing inhibitors (DMHF (2,5-Dimethyl-4-hydroxy-3-(2H)-furanone), D-Rib (d-Ribose), and D-Gal (d-Galactosamine)) were added to the co-fermentation system to further interrupt the growth of L. plantarum. It was found that 0.2 g/L DMHF or 18.0 g/L D-Gal could mainly decrease the activity of AI-2 by 38 % and 36 %, respectively, and significantly inhibit the biofilm formation of L. plantarum. Moreover, the ethanol concentrations of the co-fermentation trials added with 0.2 g/L DMHF or 18.0 g/L D-Gal were 14 % and 103 % higher than that of the control, respectively, within 30 h of fermentation. This study provides basic data for the novel prevention of L. plantarum contamination in bioethanol production via quorum sensing control, which is meaningful for the environmental protection and the sustainable development of bioethanol industry.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

AI-2 法定量感应抑制剂对减轻生物乙醇生产中细菌污染的影响

本文研究了信号分子 AI-2 在植物乳杆菌（Lactiplantibacillus plantarum）单独发酵和酿酒酵母（Saccharomyces cerevisiae）与植物乳杆菌（L. plantarum）共同发酵中的活性。研究发现，葡萄酵母和植物乳杆菌共同发酵可减少植物乳杆菌分泌 AI-2。在共发酵系统中加入了三种 AI-2 法定量感应抑制剂（DMHF（2,5-二甲基-4-羟基-3-(2H)-呋喃酮）、D-Rib（d-核糖）和 D-Gal（d-半乳糖胺）），以进一步阻断植物酵母的生长。研究发现，0.2 g/L DMHF 或 18.0 g/L D-Gal 可使 AI-2 的活性分别降低 38 % 和 36 %，并显著抑制植物酵母生物膜的形成。此外，添加了 0.2 g/L DMHF 或 18.0 g/L D-Gal 的共发酵试验在发酵 30 小时内的乙醇浓度分别比对照组高出 14 % 和 103 %。这项研究为在生物乙醇生产中通过法定量传感控制防止植物酵母污染提供了基础数据，对生物乙醇产业的环境保护和可持续发展具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Biomass & Bioenergy 工程技术-能源与燃料

CiteScore

11.50

自引率

3.30%

发文量

258

审稿时长

60 days

期刊介绍： Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials. The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy. Key areas covered by the journal: • Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation. • Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal. • Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes • Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation • Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.