Understanding the role of the fructose-1,6-bisphosphatase gene for enhancing the photosynthetic rate in Arabidopsis thaliana

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-04-19 DOI:10.1071/fp24034

Fatima Gulzar, Raza Ahmad, Suk-Yoon Kwan, Zulqurnain Khan, Sulaiman Ali Alharbi, Mohmmad Maroof Shah, Shoaib ur Rehman, Maria Siddique, Mohammad Javed Ansari, Irum Shahzadi, Muhammad Abu Bakar Saddique, Muhmmad Zahid Ishaq, Ummara Waheed

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Abstract

Transgenic Arabidopsis thaliana (ecotype Columbia) was successfully transformed with the gene fructose-1,6-bisphosphatase (FBPase) and named as AtFBPase plants. Transgenic plants exhibited stable transformation, integration and significantly higher expressions for the transformed gene. Morphological evaluation of transgenic plants showed increased plant height (35 cm), number of leaves (25), chlorophyll contents (28%), water use efficiency (increased from 1.5 to 2.6 μmol CO₂ μmol⁻¹ H₂O) and stomatal conductance (20%), which all resulted in an enhanced photosynthetic rate (2.7 μmol m⁻² s⁻¹) compared to wild type plants. This study suggests the vital role of FBPase gene in the modification of regulatory pathways to enhance the photosynthetic rate, which can also be utilised for economic crops in future.

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了解果糖-1,6-二磷酸酶基因在提高拟南芥光合速率方面的作用

转基因拟南芥（生态型哥伦比亚）成功转化了果糖-1,6-二磷酸酶（FBPase）基因，并命名为 AtFBPase 植株。转基因植株表现出稳定的转化和整合，且转化基因的表达量显著提高。转基因植株的形态学评估结果显示，与野生型植株相比，转基因植株的株高（35 厘米）、叶片数（25 片）、叶绿素含量（28%）、水分利用效率（从 1.5 μmol CO2 μmol-1 H2O 增加到 2.6 μmol）和气孔导度（20%）均有所增加，从而提高了光合速率（2.7 μmol m-2 s-1）。这项研究表明，FBPase 基因在改变调节途径以提高光合速率方面发挥着重要作用，未来也可用于经济作物。

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