Understanding the role of the fructose-1,6-bisphosphatase gene for enhancing the photosynthetic rate in Arabidopsis thaliana

IF 2.7 4区生物学 Q2 PLANT SCIENCES Functional Plant Biology Pub Date : 2024-04-19 DOI:10.1071/fp24034

Fatima Gulzar, Raza Ahmad, Suk-Yoon Kwan, Zulqurnain Khan, Sulaiman Ali Alharbi, Mohmmad Maroof Shah, Shoaib ur Rehman, Maria Siddique, Mohammad Javed Ansari, Irum Shahzadi, Muhammad Abu Bakar Saddique, Muhmmad Zahid Ishaq, Ummara Waheed

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Abstract

Transgenic Arabidopsis thaliana (ecotype Columbia) was successfully transformed with the gene fructose-1,6-bisphosphatase (FBPase) and named as AtFBPase plants. Transgenic plants exhibited stable transformation, integration and significantly higher expressions for the transformed gene. Morphological evaluation of transgenic plants showed increased plant height (35 cm), number of leaves (25), chlorophyll contents (28%), water use efficiency (increased from 1.5 to 2.6 μmol CO₂ μmol⁻¹ H₂O) and stomatal conductance (20%), which all resulted in an enhanced photosynthetic rate (2.7 μmol m⁻² s⁻¹) compared to wild type plants. This study suggests the vital role of FBPase gene in the modification of regulatory pathways to enhance the photosynthetic rate, which can also be utilised for economic crops in future.

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了解果糖-1,6-二磷酸酶基因在提高拟南芥光合速率方面的作用

转基因拟南芥（生态型哥伦比亚）成功转化了果糖-1,6-二磷酸酶（FBPase）基因，并命名为 AtFBPase 植株。转基因植株表现出稳定的转化和整合，且转化基因的表达量显著提高。转基因植株的形态学评估结果显示，与野生型植株相比，转基因植株的株高（35 厘米）、叶片数（25 片）、叶绿素含量（28%）、水分利用效率（从 1.5 μmol CO2 μmol-1 H2O 增加到 2.6 μmol）和气孔导度（20%）均有所增加，从而提高了光合速率（2.7 μmol m-2 s-1）。这项研究表明，FBPase 基因在改变调节途径以提高光合速率方面发挥着重要作用，未来也可用于经济作物。

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来源期刊

Functional Plant Biology 生物-植物科学

CiteScore

5.50

自引率

3.30%

发文量

156

审稿时长

1 months

期刊介绍： Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance. Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science. Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.