Antimicrobial action and cytotoxicity of hypochlorous acid obtained from an innovative electrolytic device – An in vitro study

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Archives of oral biology Pub Date : 2024-04-08 DOI:10.1016/j.archoralbio.2024.105966

Matheus Albino Souza , Mylena Lazareti Zanella , Gabriele Nichetti Vanin , Felipe Gomes Dallepiane , Camila Yasmin Monteiro Pizzi , Eduarda Rizzon Ferreira , Marciele Cristiane Spanenberg Fuhr , Nathan Mateus Piccolo , Huriel Scartazzini Palhano , Jordana da Silva Koch , Kellyn Rocca Souza , Ubirajara Maciel da Costa , Vanessa Valgas dos Santos , Liviu Steier , Charise Dallazem Bertol , José Antônio Poli de Figueiredo

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Abstract

Objective

This study evaluated the antimicrobial effect and cytotoxicity of hypochlorous acid(HClO) obtained from an innovative electrolytic device.

Design

The root canals of fifty extracted human teeth were inoculated with Enterococcus faecalis and divided into 5 groups (n = 10): DW (control); 2% chlorhexidine gel(CHX); 2.5% sodium hypochlorite(NaOCl); 250 ppm HClO and 500 ppm HClO. The counting of colony forming units evaluated the decontamination potential of each group. Cytotoxicity was evaluated after inoculation of tested protocols in fibroblastic cells for 3 min, calculating the cell viability. Specific statistical analysis was performed (α = 5%).

Results

The highest bacterial reduction was observed in experimental groups, with no statistical differences from each other (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences from each other (p < 0.05).

Conclusions

It could be concluded that HClO presented high antimicrobial activity and low cytotoxicity at both tested concentrations.

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从创新电解装置中获得的次氯酸的抗菌作用和细胞毒性--体外研究

设计将 50 颗人类拔牙的根管接种粪肠球菌，并分为 5 组（n = 10）：DW（对照组）；2% 洗必泰凝胶（CHX）；2.5% 次氯酸钠（NaOCl）；250 ppm HClO 和 500 ppm HClO。菌落形成单位计数评估了各组的去污潜力。在成纤维细胞中接种测试方案 3 分钟后，对细胞毒性进行评估，计算细胞存活率。进行了具体的统计分析（α = 5%）。结果实验组的细菌减少量最高，但各组之间无统计学差异（p > 0.05）。对照组的存活细胞数最多，其次是 250 ppm HClO 组和 500 ppm HClO 组，各组之间存在统计学差异（p < 0.05）。

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来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry