Archives of oral biology最新文献

{"title":"Effect of intratumor dendritic cell vaccination with and without chemoradiation in induced oral squamous cell carcinoma of hamsters","authors":"Nouran Mohamed Amr ,&nbsp;Mohamed Labib Salem ,&nbsp;Mohamed Monai Shoshan ,&nbsp;Basant Hamdy Abouzaid","doi":"10.1016/j.archoralbio.2025.106252","DOIUrl":"10.1016/j.archoralbio.2025.106252","url":null,"abstract":"<div><h3>Objectives</h3><div>Oral squamous cell carcinoma represents an important health problem since its increasing incidence and persistent high mortality rates. The traditional therapies remain surgery and chemoradiation which have shown limited success in treating recurrent and metastatic cancers with multiple complications. Immunotherapy using dendritic cell vaccination is a rather novel approach that, yet, showed limited therapeutic efficacy in eradicating oral squamous cell carcinoma since the latter is known for its immunosuppressive microenvironment. Hence, there is pressing demand for adopting new multimodality approaches that would augment the antitumor effect of dendritic cell vaccine with lesser side effects and no relapse.</div></div><div><h3>Design</h3><div>Chemically induced oral squamous cell carcinoma model of hamster buccal pouch using 7,12-Dimethylbenz[<em>a</em>]anthracene was established. The clinically evident tumor masses were then 75 % surgically debulked. Resected tumors were manipulated to prepare dendritic cell vaccine. Animals were divided into 3 groups and subsequently subjected to different treatment approaches.</div></div><div><h3>Results</h3><div>The combination therapy of debulking surgery, chemoradiation, and intratumor dendritic cell vaccination significantly delayed tumor growth with complete regression of induced tumors. Moreover, this modality was associated with the highest survival rate (89 %) with establishment of immunological memory.</div></div><div><h3>Conclusions</h3><div>Our findings emphasize the importance of modifying post-surgical environment, via co-administration of adjuvant chemoradiation, in generating robust anti-tumor immunologic activity. Chemoradiation and dendritic cell vaccines are available for clinical use/trial which endows this approach the merit of potential clinical application.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106252"},"PeriodicalIF":2.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of NET markers in experimental apical periodontitis induced in mice","authors":"Vivian Vicentin Massoni ,&nbsp;Clara Marina Pereira Cavalcanti Silva ,&nbsp;Lisa Danielly Curcino Araujo ,&nbsp;Ricardo Barbosa Lima ,&nbsp;Aluísio Eustáquio de Freitas Miranda-Filho ,&nbsp;Ana Paula Gomes-Moura ,&nbsp;Angélica Aparecida de Oliveira Denadai ,&nbsp;Carolina Maschietto Pucinelli ,&nbsp;Marília Pacífico Lucisano ,&nbsp;Paulo Nelson-Filho ,&nbsp;Alberto Consolaro ,&nbsp;Raquel Assed Bezerra da Silva ,&nbsp;Léa Assed Bezerra da Silva","doi":"10.1016/j.archoralbio.2025.106255","DOIUrl":"10.1016/j.archoralbio.2025.106255","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the expression of neutrophil extracellular trap (NET) markers in experimental apical periodontitis (AP) induced in mice, focusing on protein arginine deiminase 4 (PAD4), cathepsin G (CatG), myeloperoxidase (MPO), and citrullinated histone 3 (CitH3).</div></div><div><h3>Design</h3><div>AP was induced in 55 wild-type mice, randomly divided into a negative control (n = 5) and five experimental groups (n = 10) analyzed at 2, 5, 7, 21, and 42 days after pulp exposure of first lower molars to the oral environment. Histopathological and histomorphometric analyses included neutrophil counting and periapical lesion size measurement. NET marker expression was assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunostaining using the immunoperoxidase technique. Comparative analyses were performed with a significance level of 5 %.</div></div><div><h3>Results</h3><div>AP development was confirmed by tissue inflammation progression, pulp necrosis, and periapical bone resorption. Periapical lesion size significantly increased at 7, 21, and 42 days (p &lt; 0.001). Neutrophil counts peaked significantly at 5, 7, 21, and 42 days (p &lt; 0.001). Gene expression of all NET markers was significantly higher after 5 days (p &lt; 0.001). Immunostaining scores indicated higher MPO/CitH3 co-localization at 5, 21, and 42 days (p &lt; 0.001), PAD4 expression at 5, 7, 21, and 42 days (p &lt; 0.001), and CatG expression at 2, 7, 21, and 42 days (p &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>Experimental AP induction increased the expression of NET markers in periapical tissues in mice, suggesting a dynamic involvement in acute and chronic inflammation stages.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106255"},"PeriodicalIF":2.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of clindamycin on human osteoblasts treated with zoledronate: An in vitro study","authors":"Hunayda Bumedien-Abdelgani ,&nbsp;Francisco Javier Manzano-Moreno ,&nbsp;Anabel González-Acedo ,&nbsp;Enrique García-Recio ,&nbsp;Concepción Ruiz ,&nbsp;Elvira de Luna-Bertos","doi":"10.1016/j.archoralbio.2025.106247","DOIUrl":"10.1016/j.archoralbio.2025.106247","url":null,"abstract":"<div><h3>Objective</h3><div>The objective of this study was to determine the effects of combined treatment with clindamycin and zoledronate on the growth and differentiation of cultured human osteoblasts.</div></div><div><h3>Design</h3><div>Human osteoblasts, obtained by primary culture from mandibular bone fragments, were cultured in the presence of 50 μM zoledronate, 150 μg/mL clindamycin, or the combination of both (zoledronate + clindamycin). The effect on cell proliferation was evaluated at 24 h by the MTT colorimetric method, using a spectrophotometer at 570 nm. The effect on differentiation was examined by measuring alkaline phosphatase (ALP) activity, and mineralization by the osteoblast was studied by staining with alizarin red. Real-time polymerase chain reaction (RT-PCR) was performed for gene expression analysis. Data were expressed as means±standard deviation, and analysis of variance was performed, applying Bonferroni correction when interactions were significant.</div></div><div><h3>Results</h3><div>Treatment of osteoblasts with 50 μM zoledronate significantly reduced cell proliferation and differentiation and the gene expression of certain markers <em>versus</em> controls (p &lt; 0.001). However, treatment with 150 μg/mL clindamycin significantly increased cell proliferation and differentiation and the gene expression of certain markers (p &lt; 0.05). The combination of 150 μg/mL clindamycin and 50 μM zoledronate partially counteracted the loss of osteoblast proliferative and differentiation capacity caused by zoledronate.</div></div><div><h3>Conclusion</h3><div>Treatment with low-dose clindamycin can reverse the negative impact of zoledronate on osteoblast proliferation and differentiation. Follow-up animal studies and clinical trials are needed before topical clindamycin can be considered as a possible therapeutic resource for BP-treated patients who require a GBR procedure.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106247"},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of bisabolol on glucose metabolism in salivary gland of male diabetic rats","authors":"Letícia Martins Santos ,&nbsp;Carla Renata Sipert ,&nbsp;Gabriel Soares dos Santos ,&nbsp;Gabriella Soares dos Santos ,&nbsp;Thiago José Dionísio ,&nbsp;Carlos Ferreira dos Santos ,&nbsp;Douglas Nesadal de Souza ,&nbsp;Fernando Neves Nogueira","doi":"10.1016/j.archoralbio.2025.106246","DOIUrl":"10.1016/j.archoralbio.2025.106246","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the effect of bisabolol treatment on the activity and expression of the main enzymes of the glycolytic pathway in the submandibular and parotid salivary glands of Streptozotocin (STZ)-induced diabetic rats.</div></div><div><h3>Design</h3><div>Twenty-four male Wistar rats, aged 60 days, were divided into four groups: Control, diabetic, control+Bisabolol, and diabetic+Bisabolol. A single intraperitoneal injection of Streptozotocin induced diabetes. The treated group received a daily dose of α-Bisabolol (25 mg/kg of body weight dissolved in 0.1 mL of sunflower oil) by gavage for 21 days. The untreated group received only the vehicle. All animals were euthanized 28 days after induction, and the parotid and submandibular glands were removed, frozen in liquid nitrogen, and stored at −80 °C. The expression of the main enzymes of the glycolytic pathway was analyzed through RT-qPCR. Biochemical analyses were conducted to verify Hexokinase (HK), Phosphofructokinase (PFK), Pyruvate kinase (PK), and Lactate dehydrogenase (LDH) activity. The data were statistically compared via the Student t-test and analysis of variance (ANOVA) followed by Tukey’s test, where results were considered statistically significant at p &lt; 0.05.</div></div><div><h3>Results</h3><div>In general, bisabolol restored the HK and PFK activities and also restored Hexokinase 2 (<em>Hk2</em>), Phosphofructokinase muscle isoform (<em>Pfkm</em>) and Phosphofructokinase platelet isoform (<em>Pfkp</em>) expression in the bisabolol-treated diabetic rats. The LDH activity increased in diabetic rats with bisabolol.</div></div><div><h3>Conclusion</h3><div>Bisabolol treatment restored the activity and expression of key glycolytic enzymes, suggesting that it may have therapeutic potential in mitigating the salivary glands metabolic dysregulation caused by diabetes in rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106246"},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pan-BH-3 mimetic, obatoclax, synergistically enhances cisplatin-induced apoptosis in oral squamous cell carcinoma through a mechanism that involves degradation of the pro-survival protein Mcl-1","authors":"Tianyi Gao,&nbsp;Stefania Magnano,&nbsp;Molly Quadros,&nbsp;Patricia Hannon Barroeta,&nbsp;Daniela M. Zisterer","doi":"10.1016/j.archoralbio.2025.106250","DOIUrl":"10.1016/j.archoralbio.2025.106250","url":null,"abstract":"<div><h3>Objectives</h3><div>The purpose of the study was to elucidate the role of the Bcl-2 family of proteins in mediating cisplatin resistance in oral squamous cell carcinoma (OSCC). The value of the BH3-mimetics venetoclax and obatoclax as sensitisers for cisplatin treatment in OSCC was also evaluated.</div></div><div><h3>Design</h3><div>In this study the expression levels of a series of pro- and anti-apoptotic members of the Bcl-2 family in paired cisplatin-sensitive (SCC4) and resistant (SCC4cisR) tongue squamous carcinoma cell lines were examined by western blotting. The apoptotic rate induced by cisplatin and BH3-mimetics venetoclax and obatoclax alone or in combination in OSCC was also evaluated by Annexin V/Propidium Iodide double-stained flow cytometric assays.</div></div><div><h3>Results</h3><div>Obatoclax was shown to synergistically enhance cisplatin-induced apoptosis, and this enhancement was associated with a marked degradation in pro-survival Mcl-1 and upregulation in conformationally active form of pro-apoptotic Bak.</div></div><div><h3>Conclusions</h3><div>Our study presents novel insights into the relationship between the Bcl-2 family and cisplatin efficacy in OSCC. It also demonstrates that targeted therapy with BH-3 mimetics, such as obatoclax, may represent a new strategy for OSCC therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106250"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroethanolic extract of Spondias mombin L. leaves attenuates alveolar bone loss and inflammation in a model of periodontitis induced in male Wistar rats","authors":"Ana Beatriz Costa Almeida ,&nbsp;Agnes Andrade Martins ,&nbsp;Susana Barbosa Ribeiro ,&nbsp;Valkleidson Santos de Araújo ,&nbsp;Heloísa Espínola S. Costa ,&nbsp;Maria Luiza Diniz de Sousa Lopes ,&nbsp;Caroline Addison Carvalho Xavier de Medeiros ,&nbsp;Ericka Janine Dantas Silveira ,&nbsp;Basílio Rodrigues Vieira ,&nbsp;Frederico Barbosa Sousa ,&nbsp;Luciana Ellen Dantas Costa ,&nbsp;Silvana Maria Zucolotto ,&nbsp;Bárbara Cabral ,&nbsp;Maria Raquel Cavalcanti Inácio ,&nbsp;Bruno Cesar de Vasconcelos Gurgel ,&nbsp;Raimundo Fernandes de Araújo Júnior ,&nbsp;Emily Lima Oliveira ,&nbsp;Aurigena Antunes de Araújo ,&nbsp;Ruthineia Diógenes Alves Uchoa Lins","doi":"10.1016/j.archoralbio.2025.106249","DOIUrl":"10.1016/j.archoralbio.2025.106249","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the anti-inflammatory and antiresorptive effects of hydroethanolic extract of <em>Spondias mombin</em> L. in an experimental model of periodontitis.</div></div><div><h3>Design</h3><div>Sixty-three male Wistar rats were divided into five groups: 1) Control, 2) Periodontitis, 3) Periodontitis + <em>Spondias mombin</em> L. 50 mg/kg, 4) Periodontitis + <em>Spondias mombin</em> L. 100 mg/kg, 5) Periodontitis + <em>Spondias mombin</em> L. 200 mg/kg. Periodontitis was induced through the placement of a ligature in the cervical region of the left maxillary second molar. The rats received the extract by oral gavage for 10 days and euthanasia was performed on the 11th day of the experiment. Blood samples were used for biochemical analysis, hemi-maxillae for micro-computed tomography and histological evaluation, and gingival tissue for cytokine and RT-qPCR analyses.</div></div><div><h3>Results</h3><div>Administration of the extract reduced linear alveolar bone loss at a dose of 100 mg/kg (<em>p</em> = 0.0160) and improved bone volumetric parameters, such as integrity (BV/TV%) and trabecular porosity, at doses of 100 mg/kg (<em>p</em> = 0.0180) and 200 mg/kg (<em>p</em> = 0.0117). Trabecular separation was also improved at a dose of 200 mg/kg (<em>p</em> = 0.0405). Rats treated with 200 mg/kg showed better histological parameters (<em>p</em> = 0.0274). There was a reduction in the dosage of the pro-inflammatory cytokine interleukin 1β at doses of 50 mg/kg (<em>p</em> = 0.0496) and 100 mg/kg (<em>p</em> = 0.0374).</div></div><div><h3>Conclusions</h3><div>Our findings suggest an anti-inflammatory and antiresorptive effect of the hydroethanolic extract of <em>Spondias mombin</em> L. administered to rats with periodontitis, without altering blood biochemical markers.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106249"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal and antibiofilm activity of Lippia microphylla Cham. essential oil. (Verbenaceae) on Candida albicans and human cell cytotoxicity","authors":"Jaqueline Oliveira Barreto ,&nbsp;Larissa Alves da Silva ,&nbsp;Luanna de Oliveira e Lima ,&nbsp;Paula Lima Nogueira ,&nbsp;Rafael Carlos Ferreira ,&nbsp;Heivila Monique da Silva Alexandre ,&nbsp;Anauara Lima e Silva ,&nbsp;Juan Carlos Ramos Gonçalves ,&nbsp;Marianna Vieira Sobral ,&nbsp;Ricardo Dias de Castro ,&nbsp;Yuri Mangueira do Nascimento ,&nbsp;Josean Fechine Tavares ,&nbsp;Walicyranison Plinio da Silva Rocha ,&nbsp;Felipe Queiroga Sarmento Guerra","doi":"10.1016/j.archoralbio.2025.106243","DOIUrl":"10.1016/j.archoralbio.2025.106243","url":null,"abstract":"<div><h3>Objective</h3><div>This study evaluated the antifungal activity of <em>Lippia microphylla</em> essential oil (LM-EO) on <em>Candida albicans</em>.</div></div><div><h3>Design</h3><div>In vitro assays were conducted to test LM-EO for its anti-Candida effects, antibiofilm activity, effects on the fluorescence intensity of <em>Candida albicans</em> biofilms observed via confocal microscopy, probable mechanism of action, and toxicity on human keratinocytes. Statistical analysis was performed considering α = 5 %.</div></div><div><h3>Results</h3><div>The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of LM-EO ranged from 128 µg/mL to 256 µg/mL and 256 µg/mL to 1024 µg/mL, respectively. MIC values did not change in the presence of sorbitol, whereas a 4-fold increase was observed in the presence of ergosterol, suggesting that LM-EO may act on the cell membrane. This was subsequently observed via confocal microscopy using propidium iodide markers. LM-EO reduced biofilm adherence by 66–86 % (p &lt; 0.0001) at low concentrations (256–2560 µg/mL); however, it showed toxicity in human cells.</div></div><div><h3>Conclusions</h3><div>LM-EO exhibited fungicidal activity likely through a mechanism related to ergosterol complexation but demonstrated cytotoxicity to human keratinocytes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106243"},"PeriodicalIF":2.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric and visual assessment of two-color chewing gum mixing ability test: Correlation and reference parameters in dentate subjects","authors":"Murali Srinivasan ,&nbsp;Lisa Takeshita ,&nbsp;Prachi Jain ,&nbsp;Yasmin Milhomens Moreira ,&nbsp;Martin Schimmel ,&nbsp;Claudio R. Leles","doi":"10.1016/j.archoralbio.2025.106245","DOIUrl":"10.1016/j.archoralbio.2025.106245","url":null,"abstract":"<div><h3>Objective</h3><div>This study assessed the chewing performance of dentate subjects, using mixing ability tests with two-colored chewing gums, and aimed to correlate the visual and optoelectronic measurement methods to provide a correspondence scale for predicting the standard deviation of hue (SD-Hue) values based on visual parameters and the number of chewing strokes.</div></div><div><h3>Design</h3><div>Two chewing gums were used (Hue-Check Gum® and Vivident Fruitswing®), and tests were performed with 1, 5, 10, 15, 20, 25, and 30 chewing cycles. The analysis included optoelectronic analysis to measure the level of color mixture (ViewGum software) expressed as the SD-Hue, ranging from 0 to 1, where lower values correspond to a higher level of mixture, and subjective analysis (SA) on a 5-point ordinal scale. Data analysis included bivariate correlation, definition of double-sided 90 % reference ranges, and GEE regression.</div></div><div><h3>Results</h3><div>There were significant correlations between SD-Hue and the number of chewing cycles and SA scores (p &lt; 0.001), and SA score was strongly correlated with the number of chewing cycles (p &lt; 0.001). A downward logarithmic curve for SD-Hue and SA can be observed according to the number of chewing cycles. The mixture level progressively increased by approximately 50 % for each five chewing cycles.</div></div><div><h3>Conclusion</h3><div>It was possible to correlate visual and optoelectronic methods and to establish a scale prediction of SD-Hue values based on SA and the number of chewing cycles. The color mixing measured by optoelectronic and visual methods was proportional to the number of chewing cycles. For every five cycles, the level of color mixture was reduced by 50 %, expressed in a downward logarithmic curve, independently from the chewing gum type.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106245"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effect of platelet-derived growth factor-BB on the biological activity of human mandibular bone marrow-derived mesenchymal stem cells","authors":"Yurika Oura,&nbsp;Masakazu Ishii,&nbsp;Haruka Miyata,&nbsp;Nao Ikeda,&nbsp;Tomoaki Sakurai,&nbsp;Fumio Suehiro,&nbsp;Naohiro Komabashiri,&nbsp;Masahiro Nishimura","doi":"10.1016/j.archoralbio.2025.106244","DOIUrl":"10.1016/j.archoralbio.2025.106244","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the effects of platelet-derived growth factor-BB (PDGF-BB) on the biological activities of human mandibular bone marrow-derived mesenchymal stem cells (MBMSCs).</div></div><div><h3>Design</h3><div>PDGF-BB (20 ng/mL) was used to treat MBMSCs, and its effects on their proliferation, osteogenic differentiation, and migration were evaluated. Cell proliferation was evaluated using a WST-1 assay. Osteogenic differentiation was evaluated by measuring the mineralization potential and alkaline phosphatase activity. Cell migration was evaluated using wound healing and Transwell chamber assays. Cytoskeletal reorganization and adhesion dynamics were evaluated using immunofluorescence staining. Changes in intracellular signaling in MBMSCs induced by PDGF-BB stimulation were evaluated using western blotting. Furthermore, we investigated Girdin signaling as the molecular mechanisms underlying the regulation of PDGF-BB-induced cell migration.</div></div><div><h3>Results</h3><div>PDGF-BB treatment did not affect the proliferation or osteogenic differentiation of MBMSCs. PDGF-BB promoted the migration of MBMSCs. PDGF-BB treatment enhanced F-actin filament formation and paxillin localization at the leading edge of cells. PDGF-BB treatment activated Akt signaling in MBMSCs, and the inhibition of Akt signaling effectively suppressed PDGF-BB-induced Akt activation and migration. PDGF-BB promoted the phosphorylation of Girdin in MBMSCs, and the inhibition of Akt signaling attenuated PDGF-BB-induced Girdin activation.</div></div><div><h3>Conclusion</h3><div>This study demonstrated that PDGF-BB strongly induces the migration of MBMSCs without affecting their proliferation or osteogenic differentiation. Furthermore, PDGF-BB-induced migration of MBMSCs may be mediated through the Akt/Girdin signaling pathway. These findings provide important insight into the molecular mechanisms underlying PDGF-BB-induced periodontal tissue regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106244"},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macronutrient consumption in adults and association with oral and systemic parameters: A cross-sectional study","authors":"Larisse Santos Mendonça Alves ,&nbsp;Júlia Maria Munduri ,&nbsp;Israel Lacerda ,&nbsp;Letícia Gonçalves Reis ,&nbsp;Joana Rodrigues da Silva ,&nbsp;Cristine Miron Stefani ,&nbsp;Maria do Carmo Machado Guimarães ,&nbsp;Luana Severo Alves ,&nbsp;Solange Baraldi ,&nbsp;Naile Dame-Teixeira","doi":"10.1016/j.archoralbio.2025.106241","DOIUrl":"10.1016/j.archoralbio.2025.106241","url":null,"abstract":"<div><h3>Objective</h3><div>Oral conditions, such as tooth loss and changes in taste perception, can affect the consumption of dietary macronutrients. Our aim was to study systemic and oral alterations associated with inadequate consumption of macronutrients in adults and elderly with and without type 2 diabetes mellitus (T2D).</div></div><div><h3>Design</h3><div>This cross-sectional study included 170 individuals. Sociodemographic variables and clinical parameters were collected, such as weight status, waist circumference risk, T2D, smoking history, presence of removable dentures, worsening taste, among others. Outcomes included intakes of carbohydrates, lipids, fibers, and proteins. Poisson regression models were used to estimate prevalence ratios (PR) and 95 % confidence intervals (CI).</div></div><div><h3>Results</h3><div>Individuals reporting a worsening in taste perception were nearly four times more likely to have a high carbohydrate intake (PR=3.84; 95 % CI: 1.36–10.83). Saliva flow rate was significantly associated with increased lipid intake (PR=0.21; 95 % CI: 0.05–0.89). The presence of removable dentures in both arches (PR=1.40; 95 % CI: 1.07–1.81) and high cardiovascular risk (based on waist circumference) (PR=1.86; 95 % CI: 1.06–3.26) were associated with a higher prevalence of low fiber intake. These associations with fiber were influenced by saliva flow rate. Individuals aged ≥ 60 years (PR=1.12; 95 % CI: 1.00–1.24), using removable dentures in one arch (PR=1.23; 95 % CI: 1.04–1.46) or both arches (PR=1.22; 95 % CI: 1.04–1.45) had a higher prevalence of inadequate protein intake.</div></div><div><h3>Conclusions</h3><div>Demographic factors, systemic and oral conditions were significantly associated with inadequate macronutrient intake, highlighting the essential contribution of dentistry to addressing broader systemic health issues.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106241"},"PeriodicalIF":2.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}