Ancestral TALE homeobox protein transcription factor regulates actin dynamics and cellular activities of protozoan parasite Entamoeba invadens

IF 2.6 2区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Microbiology Pub Date : 2024-04-24 DOI:10.1111/mmi.15266
Meenakshi Pandey, Shilpa Sarkar, Sudip K. Ghosh
{"title":"Ancestral TALE homeobox protein transcription factor regulates actin dynamics and cellular activities of protozoan parasite Entamoeba invadens","authors":"Meenakshi Pandey, Shilpa Sarkar, Sudip K. Ghosh","doi":"10.1111/mmi.15266","DOIUrl":null,"url":null,"abstract":"<jats:italic>Entamoeba histolytica</jats:italic> causes invasive amoebiasis, an important neglected tropical disease with a significant global health impact. The pathogenicity and survival of <jats:italic>E. histolytica</jats:italic> and its reptilian equivalent, <jats:italic>Entamoeba invadens</jats:italic>, relies on its ability to exhibit efficient motility, evade host immune responses, and exploit host resources, all of which are governed by the actin cytoskeleton remodeling. Our study demonstrates the early origin and the regulatory role of TALE homeobox protein EiHbox1 in actin‐related cellular processes. Several genes involved in different biological pathways, including actin dynamics are differentially expressed in EiHbox1 silenced cells. EiHbox1 silenced parasites showed disrupted F‐actin organization and loss of cellular polarity. EiHbox1's presence in the anterior region of migrating cells further suggests its involvement in maintaining cellular polarity. Loss of polarized morphology of EiHbox1 silenced parasites leads to altered motility from fast, directionally persistent, and highly chemotactic to slow, random, and less chemotactic, which subsequently leads to defective aggregation during encystation. EiHbox1 knockdown also resulted in a significant reduction in phagocytic capacity and poor capping response. These findings highlight the importance of EiHbox1 of <jats:italic>E. invadens</jats:italic> in governing cellular processes crucial for their survival, pathogenicity, and evasion of the host immune system.","PeriodicalId":19006,"journal":{"name":"Molecular Microbiology","volume":"27 1","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/mmi.15266","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Entamoeba histolytica causes invasive amoebiasis, an important neglected tropical disease with a significant global health impact. The pathogenicity and survival of E. histolytica and its reptilian equivalent, Entamoeba invadens, relies on its ability to exhibit efficient motility, evade host immune responses, and exploit host resources, all of which are governed by the actin cytoskeleton remodeling. Our study demonstrates the early origin and the regulatory role of TALE homeobox protein EiHbox1 in actin‐related cellular processes. Several genes involved in different biological pathways, including actin dynamics are differentially expressed in EiHbox1 silenced cells. EiHbox1 silenced parasites showed disrupted F‐actin organization and loss of cellular polarity. EiHbox1's presence in the anterior region of migrating cells further suggests its involvement in maintaining cellular polarity. Loss of polarized morphology of EiHbox1 silenced parasites leads to altered motility from fast, directionally persistent, and highly chemotactic to slow, random, and less chemotactic, which subsequently leads to defective aggregation during encystation. EiHbox1 knockdown also resulted in a significant reduction in phagocytic capacity and poor capping response. These findings highlight the importance of EiHbox1 of E. invadens in governing cellular processes crucial for their survival, pathogenicity, and evasion of the host immune system.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
祖先 TALE 同源盒蛋白转录因子调控原生动物寄生虫 Entamoeba invadens 的肌动蛋白动力学和细胞活动
组织溶解恩塔米巴虫会引起侵袭性阿米巴病,这是一种被忽视的重要热带疾病,对全球健康有重大影响。组织溶解恩塔米巴虫及其爬行动物恩塔米巴入侵者的致病性和生存依赖于其表现出高效运动、逃避宿主免疫反应和利用宿主资源的能力,而所有这些都受肌动蛋白细胞骨架重塑的支配。我们的研究证明了 TALE 同源连接器蛋白 EiHbox1 在肌动蛋白相关细胞过程中的早期起源和调控作用。在 EiHbox1 沉默的细胞中,包括肌动蛋白动力学在内的不同生物通路中的多个基因都有不同程度的表达。沉默了EiHbox1的寄生虫表现出F-肌动蛋白组织的破坏和细胞极性的丧失。EiHbox1 出现在迁移细胞的前部区域,这进一步表明它参与了细胞极性的维持。沉默了 EiHbox1 的寄生虫失去了极化形态,导致其运动能力发生改变,从快速、定向持久和高度趋化性转变为缓慢、随机和趋化性较弱,进而导致在包囊化过程中出现聚集缺陷。敲除 EiHbox1 还会导致吞噬能力显著下降和封盖反应不良。这些发现凸显了侵袭伊蚊的 EiHbox1 在管理其生存、致病性和逃避宿主免疫系统的关键细胞过程方面的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Molecular Microbiology
Molecular Microbiology 生物-生化与分子生物学
CiteScore
7.20
自引率
5.60%
发文量
132
审稿时长
1.7 months
期刊介绍: Molecular Microbiology, the leading primary journal in the microbial sciences, publishes molecular studies of Bacteria, Archaea, eukaryotic microorganisms, and their viruses. Research papers should lead to a deeper understanding of the molecular principles underlying basic physiological processes or mechanisms. Appropriate topics include gene expression and regulation, pathogenicity and virulence, physiology and metabolism, synthesis of macromolecules (proteins, nucleic acids, lipids, polysaccharides, etc), cell biology and subcellular organization, membrane biogenesis and function, traffic and transport, cell-cell communication and signalling pathways, evolution and gene transfer. Articles focused on host responses (cellular or immunological) to pathogens or on microbial ecology should be directed to our sister journals Cellular Microbiology and Environmental Microbiology, respectively.
期刊最新文献
The Complex and Challenging World of the Host–Pathogen Interaction Comparative Multi-Omics Survey Reveals Novel Specialized Metabolites and Biosynthetic Gene Clusters Under GacS Control in Pseudomonas donghuensis Strain SVBP6 Bacterial Organelles in Iron Physiology Converging Roles of the Metal Transporter SMF11 and the Ferric Reductase FRE1 in Iron Homeostasis of Candida albicans Extracellular vesicles biogenesis and uptake concepts: A comprehensive guide to studying host-pathogen communication.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1