In vivo 13C NMR studies of glucose catabolism by isolated rumen bacteria.

J P Grivet, J Stevani, G Hannequart, M Durand
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Abstract

Nuclear magnetic resonance (NMR) was used to study the metabolism of the rumen bacterial ecosystem. The fermentation of 1-13C-glucose was investigated. This system showed a transient build-up and decay of lactate labelled at the C3 position. The degradation of lactate gave rise to volatile fatty acids. These results agree with in vivo data on diets rich in available carbohydrates.

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瘤胃细菌葡萄糖分解代谢的体内13C核磁共振研究。
采用核磁共振技术对瘤胃细菌生态系统的代谢进行了研究。研究了1- 13c -葡萄糖的发酵过程。该系统显示在C3位置标记乳酸的短暂积累和衰减。乳酸的降解产生挥发性脂肪酸。这些结果与富含有效碳水化合物饮食的体内数据一致。
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