Discovering genetic determinants for cell-to-cell adhesion in two prevalent conjugative lactococcal plasmids

IF 4.8 Q1 MICROBIOLOGY Current Research in Microbial Sciences Pub Date : 2024-01-01 DOI:10.1016/j.crmicr.2024.100239
Guillermo Ortiz Charneco , Philip Kelleher , Andrius Buivydas , Paul P. de Waal , Irma M.H. van Rijswijck , Noël N.M.E. van Peij , Christian Cambillau , Jennifer Mahony , Douwe Van Sinderen
{"title":"Discovering genetic determinants for cell-to-cell adhesion in two prevalent conjugative lactococcal plasmids","authors":"Guillermo Ortiz Charneco ,&nbsp;Philip Kelleher ,&nbsp;Andrius Buivydas ,&nbsp;Paul P. de Waal ,&nbsp;Irma M.H. van Rijswijck ,&nbsp;Noël N.M.E. van Peij ,&nbsp;Christian Cambillau ,&nbsp;Jennifer Mahony ,&nbsp;Douwe Van Sinderen","doi":"10.1016/j.crmicr.2024.100239","DOIUrl":null,"url":null,"abstract":"<div><p>Plasmids pNP40 and pUC11B encode two prevalent yet divergent conjugation systems, which have been characterized in detail recently. Here, we report the elucidation of the putative adhesins of the pNP40 and pUC11B conjugation systems, encoded by <em>traAd</em> and <em>trsAd</em>, respectively. Despite their significant sequence divergence, TraAd and TrsAd represent the most conserved component between the pNP40- and the pUC11B-encoded conjugation systems and share similar peptidoglycan-hydrolase domains. Protein structure prediction using AlphaFold2 highlighted the structural similarities between their predicted domains, as well as the potential homo-dimeric state of both proteins. Expression of the putative surface adhesins resulted in a cell clumping phenotype not only among cells expressing these surface adhesins but also between adhesin-expressing and non-producing cells. Furthermore, mutant derivatives of plasmids pNP40 or pUC11B carrying a mutation in <em>traAd</em> or <em>trsAd</em>, respectively, were shown to act as efficient donors provided the corresponding recipient expresses either <em>traAd</em> or <em>trsAd</em>, thus demonstrating <em>in trans</em> reciprocal complementarity of these proteins in conjugation systems.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100239"},"PeriodicalIF":4.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266651742400021X/pdfft?md5=f15688bd46d58f4761c3bd7135735760&pid=1-s2.0-S266651742400021X-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Research in Microbial Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S266651742400021X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Plasmids pNP40 and pUC11B encode two prevalent yet divergent conjugation systems, which have been characterized in detail recently. Here, we report the elucidation of the putative adhesins of the pNP40 and pUC11B conjugation systems, encoded by traAd and trsAd, respectively. Despite their significant sequence divergence, TraAd and TrsAd represent the most conserved component between the pNP40- and the pUC11B-encoded conjugation systems and share similar peptidoglycan-hydrolase domains. Protein structure prediction using AlphaFold2 highlighted the structural similarities between their predicted domains, as well as the potential homo-dimeric state of both proteins. Expression of the putative surface adhesins resulted in a cell clumping phenotype not only among cells expressing these surface adhesins but also between adhesin-expressing and non-producing cells. Furthermore, mutant derivatives of plasmids pNP40 or pUC11B carrying a mutation in traAd or trsAd, respectively, were shown to act as efficient donors provided the corresponding recipient expresses either traAd or trsAd, thus demonstrating in trans reciprocal complementarity of these proteins in conjugation systems.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
在两种流行的共轭乳球菌质粒中发现细胞间粘附的遗传决定因素
质粒 pNP40 和 pUC11B 编码两种普遍存在但又存在差异的连接系统,最近对这两种系统进行了详细表征。在这里,我们报告了分别由 traAd 和 trsAd 编码的 pNP40 和 pUC11B 连接系统的假定粘附素的阐明。尽管它们的序列差异很大,但 TraAd 和 TrsAd 代表了 pNP40 和 pUC11B 连接系统之间最保守的成分,并且具有相似的肽聚糖水解酶结构域。利用 AlphaFold2 进行的蛋白质结构预测突显了其预测结构域之间的结构相似性,以及这两种蛋白质潜在的同源二聚体状态。表达推测的表面粘附蛋白不仅会在表达这些表面粘附蛋白的细胞之间,而且会在表达粘附蛋白的细胞和不表达粘附蛋白的细胞之间产生细胞聚集表型。此外,分别携带 traAd 或 trsAd 突变的质粒 pNP40 或 pUC11B 的突变衍生物在相应的受体表达 traAd 或 trsAd 的情况下可作为有效的供体,从而证明了这些蛋白在共轭体系中的反式互补性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Current Research in Microbial Sciences
Current Research in Microbial Sciences Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
7.90
自引率
0.00%
发文量
81
审稿时长
66 days
期刊最新文献
Table of Contents Diverse roles of low-molecular weight thiol GSH in Francisella’s virulence, location sensing and GSH-stealing from host Acridones as promising drug candidates against Oropouche virus Genomic investigation unveils high-risk ESBL producing Enterobacteriaceae within a rural environmental water body Effects of temperature-related changes on charred bone in soil: From P release to microbial community
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1