Current Research in Microbial Sciences最新文献

Reflections on microbial genetic resources in agricultural systems

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100337

Iván Pável Moreno-Espíndola, Alonso Gutiérrez-Navarro, Diana Carolina Franco-Vásquez, Daniel Vega-Martínez

This paper reflects on the use of microbial genetic resources in the context of agricultural systems. The benefits of harnessing the diversity of these resources in any agricultural system are highlighted, as well as the importance of knowing and preserving native agrobiodiversity, which is deemed an essential resource for Latin America. In this region, harmful effects of irrational use of agrochemicals and monocultures on the environment, economy, and health have been brought to light. In view of the growing awareness and global interest in the development of bioinputs, rational biodiversity use, environmental crisis, cost of conventional synthetic inputs, and the right to safe and quality food, agriculture requires new tools and effective strategies in its practice. Microbial genetic resources are a potential source of relevant and suitable inputs for this purpose. Using the bibliometric technique of co-occurrence of terms with the VOSviewer tool, an analysis of 60 articles published between 2020 and 2024, collected from databases such as Scopus and Web of Science, was performed. An interest in microbial resources and their potential application in plant nutrition, production of growth regulators, defenses against pests and diseases, and tolerance to limiting environmental conditions can be appreciated. However, elucidating their complex ecological dynamics is necessary to understand them in real production contexts, thus allowing the allocation of relevant technological packages and the fair management of their use and potential benefits.

{"title":"Reflections on microbial genetic resources in agricultural systems","authors":"Iván Pável Moreno-Espíndola, Alonso Gutiérrez-Navarro, Diana Carolina Franco-Vásquez, Daniel Vega-Martínez","doi":"10.1016/j.crmicr.2024.100337","DOIUrl":"10.1016/j.crmicr.2024.100337","url":null,"abstract":"<div><div>This paper reflects on the use of microbial genetic resources in the context of agricultural systems. The benefits of harnessing the diversity of these resources in any agricultural system are highlighted, as well as the importance of knowing and preserving native agrobiodiversity, which is deemed an essential resource for Latin America. In this region, harmful effects of irrational use of agrochemicals and monocultures on the environment, economy, and health have been brought to light. In view of the growing awareness and global interest in the development of bioinputs, rational biodiversity use, environmental crisis, cost of conventional synthetic inputs, and the right to safe and quality food, agriculture requires new tools and effective strategies in its practice. Microbial genetic resources are a potential source of relevant and suitable inputs for this purpose. Using the bibliometric technique of co-occurrence of terms with the VOSviewer tool, an analysis of 60 articles published between 2020 and 2024, collected from databases such as Scopus and Web of Science, was performed. An interest in microbial resources and their potential application in plant nutrition, production of growth regulators, defenses against pests and diseases, and tolerance to limiting environmental conditions can be appreciated. However, elucidating their complex ecological dynamics is necessary to understand them in real production contexts, thus allowing the allocation of relevant technological packages and the fair management of their use and potential benefits.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100337"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process optimization and evaluation of quality properties of natto with co-culture of Bacillus subtilis natto and Limosilactobacillus fermentum

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100347

Yi-Ni Ma , Xun Xu , Lai-Feng Chen , Jin-Ping Zhou , Zhen-Hui Cao , Qiu-Ye Lin

The present study aimed to optimize the processing technology of natto fermented with Bacillus subtilis natto and Limosilactobacillus fermentum 2–2 and its effect on quality characteristics as well as volatile compounds. The results of single-factor tests and response surface methodology (RSM) showed that the optimal fermentation conditions of natto were L. fermentum inoculation amount of 0.3 %, fermentation temperature at 39 °C, fermentation time of 48 h, and after-ripening time of 24 h. Compared with natto control with B. subtilis natto only, natto co-cultured with B. subtilis natto and L. fermentum 2–2 showed higher scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and greater nattokinase activity. Volatomics analysis revealed that 71 differential volatile compounds were identified with 23 up-regulated and 48 down-regulated. Especially, co-fermentation of B. subtilis natto and L. fermentum 2–2 increased generation of alcohols, ketones and esters while reduced pyrazines formation. The above results indicate that co-culture of B. subtilis natto and L. fermentum can enhance the antioxidant and nattokinase activity and modify the volatile profile in natto and have the potential for application as the mixed starter in natto production.

{"title":"Process optimization and evaluation of quality properties of natto with co-culture of Bacillus subtilis natto and Limosilactobacillus fermentum","authors":"Yi-Ni Ma , Xun Xu , Lai-Feng Chen , Jin-Ping Zhou , Zhen-Hui Cao , Qiu-Ye Lin","doi":"10.1016/j.crmicr.2025.100347","DOIUrl":"10.1016/j.crmicr.2025.100347","url":null,"abstract":"<div><div>The present study aimed to optimize the processing technology of natto fermented with <em>Bacillus subtilis</em> natto and <em>Limosilactobacillus fermentum</em> 2–2 and its effect on quality characteristics as well as volatile compounds. The results of single-factor tests and response surface methodology (RSM) showed that the optimal fermentation conditions of natto were L. <em>fermentum</em> inoculation amount of 0.3 %, fermentation temperature at 39 °C, fermentation time of 48 h, and after-ripening time of 24 h. Compared with natto control with <em>B. subtilis</em> natto only, natto co-cultured with <em>B. subtilis</em> natto and L. <em>fermentum</em> 2–2 showed higher scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and greater nattokinase activity. Volatomics analysis revealed that 71 differential volatile compounds were identified with 23 up-regulated and 48 down-regulated. Especially, co-fermentation of <em>B. subtilis</em> natto and L. <em>fermentum</em> 2–2 increased generation of alcohols, ketones and esters while reduced pyrazines formation. The above results indicate that co-culture of <em>B. subtilis</em> natto and L. <em>fermentum</em> can enhance the antioxidant and nattokinase activity and modify the volatile profile in natto and have the potential for application as the mixed starter in natto production.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100347"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100350

Nattarika Chaichana , Thunchanok Yaikhan , Mingkwan Yingkajorn , Nonthawat Thepsimanon , Sirikan Suwannasin , Kamonnut Singkhamanan , Sarunyou Chusri , Rattanaruji Pomwised , Monwadee Wonglapsuwan , Komwit Surachat

Mangrovibacter phragmitis is a Gram-negative bacterium typically found in plant roots that supports nitrogen fixation in nutrient-poor environments such as mangrove ecosystems. Although primarily found in environmental niches, an unusual case in Thailand of M. phragmitis strain PSU-3885–11 isolated from the sputum of a 29-year-old female patient with spinal tuberculosis. This isolate was initially misidentified as part of the Enterobacter cloacae complex (ECC) by MALDI-TOF. However, WGS subsequently confirmed its correct identity as M. phragmitis. The genome contains 4,651 coding sequences, along with 72 tRNA genes and 1 tmRNA. Moreover, comparative genomic analysis showed 99.32 % average nucleotide identity (ANI) similar to M. phragmitis MP23, and several antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) were identified in the PSU-3885–11 genome which may contribute to its ability to survive in diverse environments, including human hosts. The PSU-3885–11 displayed resistance to beta-lactam antibiotics such as ampicillin and cefotaxime, while remaining sensitive to a wide range of other antibiotics. Key virulence genes including ompA, hcp/tssD, and rpoS, were identified which may play a role in its persistence in human hosts as an opportunistic pathogen. The presence of ribosomally synthesized and post-translationally modified peptides (RiPPs) and bacteriocins indicates the antimicrobial properties that may provide a competitive advantage in both environmental and clinical settings of this strain. Therefore, this study provides valuable insights into the genomic features, antibiotic resistance, and potential pathogenicity of M. phragmitis PSU-3885–11. The findings also emphasize the importance of continued surveillance and genomic analysis of environmental bacteria that may emerge as opportunistic pathogens in human infections.

{"title":"First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand","authors":"Nattarika Chaichana , Thunchanok Yaikhan , Mingkwan Yingkajorn , Nonthawat Thepsimanon , Sirikan Suwannasin , Kamonnut Singkhamanan , Sarunyou Chusri , Rattanaruji Pomwised , Monwadee Wonglapsuwan , Komwit Surachat","doi":"10.1016/j.crmicr.2025.100350","DOIUrl":"10.1016/j.crmicr.2025.100350","url":null,"abstract":"<div><div><em>Mangrovibacter phragmitis</em> is a Gram-negative bacterium typically found in plant roots that supports nitrogen fixation in nutrient-poor environments such as mangrove ecosystems. Although primarily found in environmental niches, an unusual case in Thailand of <em>M. phragmitis</em> strain PSU-3885–11 isolated from the sputum of a 29-year-old female patient with spinal tuberculosis. This isolate was initially misidentified as part of the <em>Enterobacter cloacae</em> complex (ECC) by MALDI-TOF. However, WGS subsequently confirmed its correct identity as <em>M. phragmitis</em>. The genome contains 4,651 coding sequences, along with 72 tRNA genes and 1 tmRNA. Moreover, comparative genomic analysis showed 99.32 % average nucleotide identity (ANI) similar to <em>M. phragmitis</em> MP23, and several antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) were identified in the PSU-3885–11 genome which may contribute to its ability to survive in diverse environments, including human hosts. The PSU-3885–11 displayed resistance to beta-lactam antibiotics such as ampicillin and cefotaxime, while remaining sensitive to a wide range of other antibiotics. Key virulence genes including <em>ompA, hcp/tssD,</em> and <em>rpoS</em>, were identified which may play a role in its persistence in human hosts as an opportunistic pathogen. The presence of ribosomally synthesized and post-translationally modified peptides (RiPPs) and bacteriocins indicates the antimicrobial properties that may provide a competitive advantage in both environmental and clinical settings of this strain. Therefore, this study provides valuable insights into the genomic features, antibiotic resistance, and potential pathogenicity of <em>M. phragmitis</em> PSU-3885–11. The findings also emphasize the importance of continued surveillance and genomic analysis of environmental bacteria that may emerge as opportunistic pathogens in human infections.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100350"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multidrug resistant Acinetobacter baumannii: A study on its pathogenesis and therapeutics 耐多药鲍曼不动杆菌的发病机制及治疗研究。

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100331

Hridesh Mukhopadhyay , Arnab Bairagi , Anushka Mukherjee , Aman Kumar Prasad , Arjama Dhar Roy , Aditi Nayak

The overuse of antibiotics has led to the global dissemination of Acinetobacter baumannii, an increasingly challenging nosocomial pathogen. This review explores the medical significance along with the diverse resistance ability of A. baumannii. Intensive care units (ICUs) serve as a breeding ground for A. baumannii, as these settings harbour vulnerable patients and facilitate the spread of opportunistic microorganisms. A. baumannii belongs to the ESKAPE group of bacterial pathogens that are major contributors to antibiotic-resistant infections. The pathogenic nature of A. baumannii is particularly evident in seriously ill patients, causing pneumonia, wound infections, and other healthcare-associated infections. Historically considered benign, A. baumannii is a global threat due to its propensity for rapid acquisition of multidrug resistance phenotypes. The genus Acinetobacter was formally recognized in 1968 following a comprehensive survey by Baumann et al., highlighting the relationship between previously identified species and consolidating them under the name Acinetobacter. A. baumannii is characterized by its Gram-negative nature, dependence on oxygen, positive catalase activity, lack of oxidase activity, inability to ferment sugars, and non-motility. The DNA G+C content of Acinetobacter species falls within a specific range. For diagnostic purposes, A. baumannii can be cultured on specific agar media, producing distinct colonies. The genus Acinetobacter comprises numerous species those are associated with bloodstream infections with high mortality rates. Therefore, A. baumannii poses a significant challenge to global healthcare due to its multidrug resistance and ability to cause various infections. A comprehensive understanding of the mechanisms underlying its resistance acquisition and pathogenicity is essential for combating this healthcare-associated pathogen effectively.

抗生素的过度使用导致鲍曼不动杆菌的全球传播，这是一种越来越具有挑战性的医院病原体。本文就鲍曼不动杆菌耐药能力的多样性及其医学意义作一综述。重症监护病房（icu）是鲍曼不动杆菌的滋生地，因为这些环境庇护着脆弱的病人，并促进了机会性微生物的传播。鲍曼不动杆菌属于ESKAPE细菌病原体群，是抗生素耐药感染的主要贡献者。鲍曼不动杆菌的致病性在重症患者中尤为明显，可引起肺炎、伤口感染和其他卫生保健相关感染。历史上被认为是良性的鲍曼不动杆菌由于其快速获得多药耐药表型的倾向而成为全球威胁。1968年，在Baumann等人的全面调查后，Acinetobacter属被正式确认，强调了先前确定的物种之间的关系，并将它们合并为Acinetobacter。鲍曼不动杆菌的特点是革兰氏阴性、依赖氧气、过氧化氢酶活性阳性、缺乏氧化酶活性、不能发酵糖和不运动。不动杆菌的DNA G+C含量在一定范围内。为了诊断目的，鲍曼芽胞杆菌可以在特定的琼脂培养基上培养，产生不同的菌落。不动杆菌属包括许多与高死亡率的血流感染有关的物种。因此，鲍曼不动杆菌由于其多药耐药性和引起各种感染的能力，对全球医疗保健构成了重大挑战。全面了解其耐药性获得和致病性的机制对于有效地对抗这种卫生保健相关病原体至关重要。

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引用次数: 0

Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100366

Catherine Dunyach-Remy , Cassandra Pouget , Yves-Marie Pers , Cécile Gaujoux-Viala , Christophe Demattei , Florian Salipante , Lucia Grenga , Jean Armengaud , Jean-Philippe Lavigne , Christian Jorgensen

The objective of this study was to investigate the link between gut microbiota (GM) dysbiosis, gut inflammation, and bacterial translocation (BT) in recently diagnosed rheumatoid arthritis (RA). This case-control, observational study prospectively recruited recently diagnosed (<12 months) RA patients and age-matched healthy controls (HC) from two French hospitals between July 2014 to March 2018. The primary objective was to investigate GM composition in each group using 16S rRNA sequencing and metaproteomics approaches. Three plasmatic BT markers (sCD14, LPS-binding protein, and number of 16S rRNA gene copies) and one intestinal permeability marker (I-FABP) were quantified in blood samples.

Twenty-five were included in each group, and 50 stools and blood samples were analyzed. 16S rRNA gene analysis showed an decrease in Coprococcus in RA patients after Body Mass Index and HLA status. Circulating bacterial DNA (number of copies of the 16S rRNA gene) and plasmatic I-FABP were higher in RA patients compared to HCs (p < 0.01), indicating increased BT and intestinal permeability in these patients. Metaproteomics from stool samples highlighted an increased host humoral immune response in RA, with elevated levels of inflammatory proteins (azurocidin, cathepsin G, neutrophil defensing 1). Gut inflammation may contribute to increased intestinal permeability, leading to BT into the systemic circulation and thus chronic inflammation.

{"title":"Participation of gut microbiota and bacterial translocation in chronic systemic inflammation in recently diagnosed rheumatoid arthritis patients","authors":"Catherine Dunyach-Remy , Cassandra Pouget , Yves-Marie Pers , Cécile Gaujoux-Viala , Christophe Demattei , Florian Salipante , Lucia Grenga , Jean Armengaud , Jean-Philippe Lavigne , Christian Jorgensen","doi":"10.1016/j.crmicr.2025.100366","DOIUrl":"10.1016/j.crmicr.2025.100366","url":null,"abstract":"<div><div>The objective of this study was to investigate the link between gut microbiota (GM) dysbiosis, gut inflammation, and bacterial translocation (BT) in recently diagnosed rheumatoid arthritis (RA). This case-control, observational study prospectively recruited recently diagnosed (<12 months) RA patients and age-matched healthy controls (HC) from two French hospitals between July 2014 to March 2018. The primary objective was to investigate GM composition in each group using 16S rRNA sequencing and metaproteomics approaches. Three plasmatic BT markers (sCD14, LPS-binding protein, and number of 16S rRNA gene copies) and one intestinal permeability marker (I-FABP) were quantified in blood samples.</div><div>Twenty-five were included in each group, and 50 stools and blood samples were analyzed. 16S rRNA gene analysis showed an decrease in <em>Coprococcus</em> in RA patients after Body Mass Index and HLA status. Circulating bacterial DNA (number of copies of the 16S rRNA gene) and plasmatic I-FABP were higher in RA patients compared to HCs (<em>p</em> < 0.01), indicating increased BT and intestinal permeability in these patients. Metaproteomics from stool samples highlighted an increased host humoral immune response in RA, with elevated levels of inflammatory proteins (azurocidin, cathepsin G, neutrophil defensing 1). Gut inflammation may contribute to increased intestinal permeability, leading to BT into the systemic circulation and thus chronic inflammation.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100366"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in research on gut microbiota and allergic diseases in children

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100362

Heng Ke, Hongbing Yao, Ping Wei

Epidemiological studies indicate a rising prevalence of allergic diseases, now recognized as a major global public health concern. In children, the progression of these diseases often follows the "atopic march," beginning with eczema, followed by food allergies, allergic rhinitis, and asthma. Recent research has linked gut microbiota dysbiosis to the development of allergic diseases in children. The gut microbiota, a crucial component of human health, plays a vital role in maintaining overall well-being, highlighting its potential in preventing and modifying the course of allergic diseases. This review examines the relationship between childhood allergic diseases and gut microbiota, drawing on the latest evidence. We first elaborated the concepts of allergic diseases and gut microbiota, followed by a discussion of the developmental trajectory of the gut microbiota in healthy children. This review further explored the richness, diversity, and composition of the gut microbiota, as well as specific microbial taxa associated with allergic disease. Lastly, we discussed the current status and future potential of probiotic interventions in managing pediatric allergic diseases.

{"title":"Advances in research on gut microbiota and allergic diseases in children","authors":"Heng Ke, Hongbing Yao, Ping Wei","doi":"10.1016/j.crmicr.2025.100362","DOIUrl":"10.1016/j.crmicr.2025.100362","url":null,"abstract":"<div><div>Epidemiological studies indicate a rising prevalence of allergic diseases, now recognized as a major global public health concern. In children, the progression of these diseases often follows the \"atopic march,\" beginning with eczema, followed by food allergies, allergic rhinitis, and asthma. Recent research has linked gut microbiota dysbiosis to the development of allergic diseases in children. The gut microbiota, a crucial component of human health, plays a vital role in maintaining overall well-being, highlighting its potential in preventing and modifying the course of allergic diseases. This review examines the relationship between childhood allergic diseases and gut microbiota, drawing on the latest evidence. We first elaborated the concepts of allergic diseases and gut microbiota, followed by a discussion of the developmental trajectory of the gut microbiota in healthy children. This review further explored the richness, diversity, and composition of the gut microbiota, as well as specific microbial taxa associated with allergic disease. Lastly, we discussed the current status and future potential of probiotic interventions in managing pediatric allergic diseases.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100362"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental biocontamination by SARS-CoV-2 Virus in the hospital setting

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100355

M Espejo Mambié , D San Jose-Saras , C Bischofberger Valdés , C Díaz-Agero Pérez , JC Galán Montemayor , L Martínez-García , M Abreu Di-Berardino , P Moreno-Nunez , J Vicente-Guijarro , J.M Aranaz-Andrés

Background

Demonstrating the capability to isolate biological material from the environment was fundamental to supporting any transmission route. Various and inconsistent methodologies have been used to address this issue; however, the debate in scientific societies about the possibility of airborne transmission as a source of SARS-CoV-2 spread remained open.

Objective

To analyze SARS-CoV-2 contamination in the air and on surfaces in a hospital setting during the COVID-19 pandemic.

Methods

This study involved air and surface sampling in the emergency, hospitalization, and intensive care unit areas of the Ramón y Cajal University Hospital. A consistent methodology was used for all samples, and clinical and environmental parameters and characterization of each location were recorded.

Results

A total of 234 samples were collected, comprising 160 surface samples and 74 air samples, of which 6.84 % tested positive (13/160 surface samples and 3/74 air samples). High-contact surfaces had the highest proportion of positive samples (12/13). All positive air samples were identified within 2 m of patients who had recently developed symptoms (<5 days). High dependency and elevated temperatures seemed to indicate a higher risk of environmental biocontamination. Additionally, there was a higher risk of contamination in the intensive care units than in the hospitalization or emergency units.

{"title":"Environmental biocontamination by SARS-CoV-2 Virus in the hospital setting","authors":"M Espejo Mambié , D San Jose-Saras , C Bischofberger Valdés , C Díaz-Agero Pérez , JC Galán Montemayor , L Martínez-García , M Abreu Di-Berardino , P Moreno-Nunez , J Vicente-Guijarro , J.M Aranaz-Andrés","doi":"10.1016/j.crmicr.2025.100355","DOIUrl":"10.1016/j.crmicr.2025.100355","url":null,"abstract":"<div><h3>Background</h3><div>Demonstrating the capability to isolate biological material from the environment was fundamental to supporting any transmission route. Various and inconsistent methodologies have been used to address this issue; however, the debate in scientific societies about the possibility of airborne transmission as a source of SARS-CoV-2 spread remained open.</div></div><div><h3>Objective</h3><div>To analyze SARS-CoV-2 contamination in the air and on surfaces in a hospital setting during the COVID-19 pandemic.</div></div><div><h3>Methods</h3><div>This study involved air and surface sampling in the emergency, hospitalization, and intensive care unit areas of the Ramón y Cajal University Hospital. A consistent methodology was used for all samples, and clinical and environmental parameters and characterization of each location were recorded.</div></div><div><h3>Results</h3><div>A total of 234 samples were collected, comprising 160 surface samples and 74 air samples, of which 6.84 % tested positive (13/160 surface samples and 3/74 air samples). High-contact surfaces had the highest proportion of positive samples (12/13). All positive air samples were identified within 2 m of patients who had recently developed symptoms (<5 days). High dependency and elevated temperatures seemed to indicate a higher risk of environmental biocontamination. Additionally, there was a higher risk of contamination in the intensive care units than in the hospitalization or emergency units.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100355"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genomics of Aspergillus nidulans and section Nidulantes

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100342

Sebastian Theobald , Tammi Vesth , Jane L. Nybo , Jens C. Frisvad , Inge Kjærbølling , Stephen Mondo , Kurt LaButti , Sajeet Haridas , Robert Riley , Alan A. Kuo , Asaf A. Salamov , Jasmyn Pangilinan , Anna Lipzen , Maxim Koriabine , Mi Yan , Kerrie Barry , Alicia Clum , Ellen K. Lyhne , Elodie Drula , Ad Wiebenga , Mikael R. Andersen

Aspergillus nidulans is an important model organism for eukaryotic biology and the reference for the section Nidulantes in comparative studies. In this study, we de novo sequenced the genomes of 25 species of this section. Whole-genome phylogeny of 34 Aspergillus species and Penicillium chrysogenum clarifies the position of clades inside section Nidulantes. Comparative genomics reveals a high genetic diversity between species with 684 up to 2433 unique protein families. Furthermore, we categorized 2118 secondary metabolite gene clusters (SMGC) into 603 families across Aspergilli, with at least 40 % of the families shared between Nidulantes species. Genetic dereplication of SMGC and subsequent synteny analysis provides evidence for horizontal gene transfer of a SMGC. Proteins that have been investigated in A. nidulans as well as its SMGC families are generally present in the section Nidulantes, supporting its role as model organism. The set of genes encoding plant biomass-related CAZymes is highly conserved in section Nidulantes, while there is remarkable diversity of organization of MAT-loci both within and between the different clades. This study provides a deeper understanding of the genomic conservation and diversity of this section and supports the position of A. nidulans as a reference species for cell biology.

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引用次数: 0

Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100349

Marianna Marangi , Sonia Boughattas

Blastocystis sp. is frequently identified in humans and several animal hosts exhibiting a wide genetic diversity. Within One Health perspective, data on Blastocystis sp. distribution and its circulating subtypes (STs) from the terrestrial environment are available, while those from the marine environment remain still scare. A genetic and 16S rRNA gene sequencing analysis were conducted over the period 2022–2024 by screening fecal samples from four different species of free-ranging marine mammals (sperm, fin, long-finned pilot and Cuvier's beaked whales) circulating within North-Western Mediterranean Sea. 10 out of 43 fecal samples (23.2 %) were found positive to Blastocystis sp. using molecular tools. A predominance of zoonotic subtype ST3 among different species of marine mammals as well as the presence of ST1 allele 4 subtype and even untypable subtype within the fin whale specimen was reported. Moreover, Firmicutes, Bacteroidetes and Proteobacteria within the different Blastocystis-carrier marine mammal species as well the identification of Archaebacteria from Methanomethylophilaceae family within the fin whale isolate were detected by Illumina V3-V4 generated data. The present survey presents new insights regarding Blastocystis sp. prevalence and its circulating zoonotic ST1-ST3 subtypes from the marine environment, as well as its associated gut microbiome, providing hence baseline data for a better understanding of the associated risk and to prevent human and marine ecosystem exposure to these anthropogenic microorganisms.

{"title":"Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea","authors":"Marianna Marangi , Sonia Boughattas","doi":"10.1016/j.crmicr.2025.100349","DOIUrl":"10.1016/j.crmicr.2025.100349","url":null,"abstract":"<div><div><em>Blastocystis</em> sp. is frequently identified in humans and several animal hosts exhibiting a wide genetic diversity. Within One Health perspective, data on <em>Blastocystis</em> sp. distribution and its circulating subtypes (STs) from the terrestrial environment are available, while those from the marine environment remain still scare. A genetic and 16S rRNA gene sequencing analysis were conducted over the period 2022–2024 by screening fecal samples from four different species of free-ranging marine mammals (sperm, fin, long-finned pilot and Cuvier's beaked whales) circulating within North-Western Mediterranean Sea. 10 out of 43 fecal samples (23.2 %) were found positive to <em>Blastocystis</em> sp. using molecular tools. A predominance of zoonotic subtype ST3 among different species of marine mammals as well as the presence of ST1 allele 4 subtype and even untypable subtype within the fin whale specimen was reported. Moreover, Firmicutes, Bacteroidetes and Proteobacteria within the different <em>Blastocystis</em>-carrier marine mammal species as well the identification of Archaebacteria from Methanomethylophilaceae family within the fin whale isolate were detected by Illumina V3-V4 generated data. The present survey presents new insights regarding <em>Blastocystis</em> sp. prevalence and its circulating zoonotic ST1-ST3 subtypes from the marine environment, as well as its associated gut microbiome, providing hence baseline data for a better understanding of the associated risk and to prevent human and marine ecosystem exposure to these anthropogenic microorganisms.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100349"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial potential and phytochemical analysis of two ethnomedicinally important plants 两种重要民族药材的抑菌潜力及植物化学分析。

IF 4.8 Q1 MICROBIOLOGY

Current Research in Microbial Sciences

Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100297

Narpat Singh, Neha Chhangani, Sharad Bissa

Medicinal plants exhibited great role in drug industries. Herbal medicines and their derivative products are often prepared from crude plant extracts. Echinops echinatus and Tridax procumbens both are belonging to Asteraceae family and these plants are ethnomedicinally important due to their utilization as traditional medicine to cure various diseases. Aim of the current study is to evaluate the antimicrobial properties, preliminary phytochemical and GC-MS analysis of these ethnomedicinally important plants to identify the compounds which are responsible for antimicrobial properties. Their extracts exhibited antimicrobial activity against Enterobacter aerogenes, Escherichia coli, Agrobacterium tumefaciens, Staphylococcus aureus, Bacillus subtilis, Pseudomonas syringae and Pseudomonas putida. Both plants contain the active principles like flavonoids, alkaloids, glycosides, saponins, terpenoids and tannins. Result of GC-MS analysis showed the presence of many compounds such as n-Hexadecanoic acid, Hexadecanoic Acid, Methyl ester, Octadecanoic acid, Stigmasterol, Naphthalene, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-,5 Hydroxymethylfurfural, Lupeol, Dodecanoic acid, Vitamin E (α-Tocopherol), Neophytadiene, Phytol and many other compounds. These compounds are responsible for antimicrobial, anticancer and medicinal properties.

药用植物在医药工业中发挥了巨大的作用。草药及其衍生物通常由植物粗提取物制成。棘球菊和棘球菊都是菊科植物，作为传统药材治疗多种疾病，具有重要的民族医学意义。本研究的目的是对这些民族医学上重要的植物的抗菌特性进行评价，初步的植物化学和GC-MS分析，以确定具有抗菌特性的化合物。其提取物对产气肠杆菌、大肠杆菌、农杆菌、金黄色葡萄球菌、枯草芽孢杆菌、丁香假单胞菌和恶臭假单胞菌均有抑菌活性。这两种植物都含有黄酮类、生物碱、糖苷、皂苷、萜类和单宁等活性成分。气相色谱-质谱分析结果显示，样品中含有正癸酸、癸酸、甲酯、十八烷酸、豆甾醇、萘、角鲨烯、4h -吡喃-4- 1,2,3 -二氢-3,5-二羟基-6-甲基-、角鲨烯、4h -吡喃-4- 1,2,3 -二氢-3,5-二羟基-6-甲基-、5羟甲基糠醛、Lupeol、十二烷酸、维生素E （α-生育酚）、新植物烯、叶绿醇等多种化合物。这些化合物具有抗菌、抗癌和药用特性。

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引用次数: 0