Application of conjunctival graft surgery in the treatment of corneal ulcers in dogs

Tan N. Nguyen, Ba Q. Phan, Thuong T. Nguyen
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Abstract

The study of conjunctival graft surgery for treating corneal ulcers in dogs was conducted at Alpha Pet Veterinary Clinic and Bang Pham Veterinary Hospital from August 2021 to August 2022. Dogs were clinically examined with corneal ulcers and corneal perforations, corneal reflexes, and then fluorescein 2% reagent was used to examine and measure the diameter of the corneal damaged area by using Castroviejo calipers. The surgical method of conjunctival stem grafting was applied and evaluated effects of treatment on corneal ulcers in dogs. A total of 3,024 dogs were examined and treated during the study period. There were 195 dogs with eye diseases, accounting for 6.45% of the cases. The rate of corneal ulcers was 15.90% (31/195 cases). Among the corneal ulcer cases, conjunctival graft surgery was performed in 14 cases. The successful rate of conjunctival stem grafting was 71.43%. The complication rate of conjunctival stem grafting was 42.86%, in which 2 cases of mild complications were successfully treated. The average wound healing duration was 39.45 days. The occurrence of complications depended on the types of complication. Mild complications including suture detachment and corneal edema appeared after 2.5 to 5.5 days while corneal epithelial cysts and ocular edema occurred at 22 to 42 days after surgery. Therefore, this method could be used in treatment of corneal ulcers, particularly in cases of deep ulcers or corneal perforations that required to directly connect to blood vessels.
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结膜移植手术在犬角膜溃疡治疗中的应用
结膜移植手术治疗犬角膜溃疡的研究于 2021 年 8 月至 2022 年 8 月在阿尔法宠物兽医诊所和 Bang Pham 兽医医院进行。临床检查犬的角膜溃疡和角膜穿孔、角膜反射,然后使用 2% 荧光素试剂检查角膜受损区域,并用 Castroviejo 卡尺测量角膜受损区域的直径。应用结膜干细胞移植手术方法,评估治疗对犬角膜溃疡的效果。在研究期间,共有 3,024 只狗接受了检查和治疗。其中 195 只狗患有眼疾,占病例总数的 6.45%。角膜溃疡的发病率为 15.90%(31/195 例)。在角膜溃疡病例中,有 14 例进行了结膜移植手术。结膜干细胞移植手术的成功率为 71.43%。结膜干细胞移植手术的并发症发生率为 42.86%,其中 2 例轻度并发症患者得到了成功治疗。伤口愈合时间平均为 39.45 天。并发症的发生取决于并发症的类型。轻度并发症包括缝线脱落和角膜水肿在术后 2.5 至 5.5 天出现,而角膜上皮囊肿和眼部水肿则在术后 22 至 42 天出现。因此,这种方法可用于治疗角膜溃疡,尤其是需要直接连接血管的深层溃疡或角膜穿孔。
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