Preparation, sustained release and cell imaging studies of rhodamine 6G@-nido-carborane fluorescent polymer

IF 2.8 4区工程技术 Q2 POLYMER SCIENCE Macromolecular Research Pub Date : 2024-04-24 DOI:10.1007/s13233-024-00252-y

Tiantian Chai, Ying Liu, Meng Zhou, Shuo Wang, Jiankang Feng, Mengtong Zhang, Xibing Feng, Jingnan Hu, Qingxia Chu, Chichong Lu, Guofan Jin

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Abstract

In this paper, rhodamine 6G and carborane were used as raw materials, and four fluorescent polymers were prepared by one-pot method, namely L100-55 fluorescent polymer (L100-55-B), EPO polymer (EPO-B), RS polymer (RS) and RL polymer (RL). The problem of poor water solubility of carborane was solved and the biocompatibility of rhodamine 6G-nido-carborane was improved. By simulating the release of polymers in different gastrointestinal environments, it was found that the release rate of drugs in the four coating materials was slow and controllable, with good bioavailability. Affected by the properties of the resin, L100-55 and EPO coating had the best release effect in the gastrointestinal tract. In the zeta potential test, the absolute potential values of L100-55-B and EPO-B are stable, both up to 30 mV. Transmission electron microscopy revealed that the drug was uniformly dispersed in the drug carrier material and wrapped into nanospheres with particle sizes below 500 nm. Hela cells were imaged in different acidic environments, and the results showed that the polymer had good affinity with target cells and was closely connected to target cells, indicating good biocompatibility and targeting of the polymer. This design not only solves the bioavailability problem of rhodamine 6G-nido-carborane, but also has a good fluorescence targeting effect.

Graphical abstract

Rhodamine 6G-nido-carborane coated by four eudragits, L100-55, EPO, RS, and RL, are released in the gastrointestinal environment. Cell imaging under a microscope shows that L100-55-B and EPO-B have a strong affinity for Hela cells. Four coating pathways, the released rhodamine 6G-nido-carborane targets tumor cells and exerts inhibitory effects.

1. The bioavailability and water solubility of carborane derivatives have been improved.

2. Rhodamine 6G is used to make the complex visible in vivo, which is convenient for monitoring the drug distribution.

3. Acrylic resin coating can change drug release performance.

4. Hela cell imaging experiments display excellent cellular permeability.

Abstract Image

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罗丹明 6G@-nido-硼烷荧光聚合物的制备、持续释放和细胞成像研究

本文以罗丹明6G和碳硼烷为原料，采用一锅法制备了四种荧光聚合物，即L100-55荧光聚合物（L100-55-B）、EPO聚合物（EPO-B）、RS聚合物（RS）和RL聚合物（RL）。解决了碳硼烷水溶性差的问题，提高了罗丹明 6G 新碳硼烷的生物相容性。通过模拟聚合物在不同胃肠道环境中的释放，发现四种包衣材料的药物释放速率缓慢且可控，具有良好的生物利用度。受树脂特性的影响，L100-55 和 EPO 包衣在胃肠道中的释放效果最好。在 zeta 电位测试中，L100-55-B 和 EPO-B 的绝对电位值比较稳定，均可达 30 mV。透射电子显微镜显示，药物均匀地分散在载药材料中，并包裹成粒径低于 500 nm 的纳米球。在不同酸性环境下对 Hela 细胞进行成像，结果表明聚合物与靶细胞具有良好的亲和性，并与靶细胞紧密相连，表明聚合物具有良好的生物相容性和靶向性。该设计不仅解决了罗丹明 6G-nido-硼烷的生物利用度问题，而且具有良好的荧光靶向效果。图文摘要罗丹明 6G-nido-硼烷包被 L100-55、EPO、RS 和 RL 四种缩水甘油酯后在胃肠道环境中释放。显微镜下的细胞成像显示，L100-55-B 和 EPO-B 对 Hela 细胞有很强的亲和力。通过四种包衣途径，释放出的罗丹明6G-nido-硼烷可靶向肿瘤细胞并发挥抑制作用。 1.提高了硼烷衍生物的生物利用度和水溶性；2.利用罗丹明6G使复合物在体内可见，便于监测药物分布；3.丙烯酸树脂包衣可改变药物释放性能；4.海拉细胞成像实验显示了良好的细胞渗透性。

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来源期刊

Macromolecular Research 工程技术-高分子科学

CiteScore

4.70

自引率

8.30%

发文量

100

审稿时长

1.3 months

期刊介绍： Original research on all aspects of polymer science, engineering and technology, including nanotechnology Presents original research articles on all aspects of polymer science, engineering and technology Coverage extends to such topics as nanotechnology, biotechnology and information technology The English-language journal of the Polymer Society of Korea Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.