Minyak Atsiri Serai Wangi (Cymbopogon nardus L. Rendle): Diisolasi dengan Dua Metode Berbeda, Kualitas dan Aktivitas Antibakterinya

Afrizal, Anggun Defitriani, Mai Efdi
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Abstract

Cymbopogon nardus L. Rendle is one of the world trades essential oil plants. The high selling value of citronella oil is determined by the characteristics of its physical and chemical properties. In this study, determined the physical and chemical properties of citronellal oil isolated by two different methods, namely method of laboratory distillation (M1) and local community distillation (M2). The result showed that the essential oil produce from both methods match with SNI 06-3953-1995 on its physical properties. The results of the chemical composition using Gas Chromatography-Mass Spectrometry (GC-MS) indicated there are 38 and 48 compounds in the essential oil of M1 and M2, respectively with the main compositions are citronellal, citronellol, and geraniol. The antibacterial activity evaluated using the disc diffusion method showed inhibitory ability against the growth of Staphylococcus aureus and Staphylococcus epidermidis, but no inhibitory ability of Pseudomonas aeruginosa. Both, these M1 and M2 essential oils are categorized as a very strong antibacterial at concentration of 3.125% and 50%, respectively.
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香茅精油(Cymbopogon nardus L. Rendle):用两种不同方法分离出的香茅精油及其质量和抗菌活性
香茅(Cymbopogon nardus L. Rendle)是世界上畅销的精油植物之一。香茅油的高销售价值取决于其物理和化学特性。在这项研究中,通过两种不同的方法,即实验室蒸馏法(M1)和当地社区蒸馏法(M2),测定了分离出来的香茅油的物理和化学特性。结果表明,两种方法分离出的香茅精油的物理特性均符合 SNI 06-3953-1995。使用气相色谱-质谱法(GC-MS)检测化学成分的结果表明,M1 和 M2 的精油中分别含有 38 和 48 种化合物,主要成分为香茅醛、香茅醇和香叶醇。采用盘扩散法评估的抗菌活性表明,它们对金黄色葡萄球菌和表皮葡萄球菌的生长具有抑制能力,但对铜绿假单胞菌没有抑制能力。M1 和 M2 精油的浓度分别为 3.125% 和 50%,都被归类为极强抗菌剂。
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