Construction of dental pulp decellularized matrix by cyclic lavation combined with mechanical stirring and its proteomic analysis.

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL Biomedical materials Pub Date : 2024-04-23 DOI:10.1088/1748-605X/ad4245

Zhijun Zhang, Fei Bi, Yibing Huang, Weihua Guo

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Abstract

The decellularized matrixhas a great potential for tissue remodeling and regeneration; however, decellularization could induce host immune rejection due to incomplete cell removal or detergent residues, thereby posing significant challenges for its clinical application. Therefore, the selection of an appropriate detergent concentration, further optimization of tissue decellularization technique, increased of biosafety in decellularized tissues, and reduction of tissue damage during the decellularization procedures are pivotal issues that need to be investigated. Inthis study, we tested several conditions and determined that 0.1% Sodium dodecyl sulfate(SDS) and three decellularization cycles were the optimal conditions for decellularization of pulp tissue. Decellularization efficiency was calculated and the preparation protocol for dental pulp decellularization matrix (DPDM) was further optimized. To characterize the optimized DPDM, the microstructure, odontogenesis-related protein and fiber content were evaluated. Our results showed that the properties of optimized DPDM were superior to those of the non-optimized matrix. We also performed the 4D-Label-free quantitative proteomic analysis of DPDM and demonstrated the preservation of proteins from the natural pulp. This study provides a solid theoretical and experimental foundation for the potential application of DPDM in pulp regeneration.

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通过循环灌洗结合机械搅拌构建牙髓脱细胞基质及其蛋白质组分析。

脱细胞基质在组织重塑和再生方面具有巨大潜力；然而，脱细胞可能会因细胞去除不彻底或洗涤剂残留而诱发宿主免疫排斥反应，从而为其临床应用带来重大挑战。因此，选择合适的洗涤剂浓度、进一步优化组织脱细胞技术、提高脱细胞组织的生物安全性以及减少脱细胞过程中的组织损伤是需要研究的关键问题。在本研究中，我们测试了几种条件，确定0.1%十二烷基硫酸钠（SDS）和三个脱细胞周期是纸浆组织脱细胞的最佳条件。通过计算脱细胞效率，进一步优化了牙髓脱细胞基质（DPDM）的制备方案。为了表征优化后的牙髓脱细胞基质，我们对其微观结构、与牙髓发生相关的蛋白质和纤维含量进行了评估。结果表明，优化后的 DPDM 性能优于未优化的基质。我们还对 DPDM 进行了 4D 无标记定量蛋白质组学分析，证明其保留了天然牙髓中的蛋白质。这项研究为 DPDM 在牙髓再生中的潜在应用提供了坚实的理论和实验基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical materials 工程技术-材料科学：生物材料

CiteScore

6.70

自引率

7.50%

发文量

294

审稿时长

3 months

期刊介绍： The goal of the journal is to publish original research findings and critical reviews that contribute to our knowledge about the composition, properties, and performance of materials for all applications relevant to human healthcare. Typical areas of interest include (but are not limited to): -Synthesis/characterization of biomedical materials- Nature-inspired synthesis/biomineralization of biomedical materials- In vitro/in vivo performance of biomedical materials- Biofabrication technologies/applications: 3D bioprinting, bioink development, bioassembly & biopatterning- Microfluidic systems (including disease models): fabrication, testing & translational applications- Tissue engineering/regenerative medicine- Interaction of molecules/cells with materials- Effects of biomaterials on stem cell behaviour- Growth factors/genes/cells incorporated into biomedical materials- Biophysical cues/biocompatibility pathways in biomedical materials performance- Clinical applications of biomedical materials for cell therapies in disease (cancer etc)- Nanomedicine, nanotoxicology and nanopathology- Pharmacokinetic considerations in drug delivery systems- Risks of contrast media in imaging systems- Biosafety aspects of gene delivery agents- Preclinical and clinical performance of implantable biomedical materials- Translational and regulatory matters