A Study of Condensates Collected during the Fermentation of Grape Must

Jakub Humaj, M. Baroň, M. Kumsta, Jiri Sochor, Pavel Pavlousek
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Abstract

This article deals with the analysis of the condensates which are formed from fermentation gases during the fermentation of grape must. The experiment was divided into two parts. In the first part, the evolution of the individual volatiles was monitored throughout the whole fermentation process of the Riesling variety. In the second part, the condensates from three different grape varieties (Riesling, Merlot, Sauvignon blanc) were investigated and the total content of the selected volatile substances was measured at the end of the fermentation. Attention was focused on the measurements using a GC-MS (gas chromatography-mass spectrometry) for the volatile substances: isoamyl alcohol, isobutyl alcohol, 1-propanol, ethyl acetate, ethyl hexanoate, ethyl octanoate, ethyl decanoate, acetaldehyde, acetic acid, and acetoin. In addition, changes in the alcohol content of the condensate, with respect to the fermentation phase, were analysed. From the results of part 1, the quantity of the substances under investigation produced during fermentation was determined. The highest concentration of flavour compounds was during the fourth and fifth days of fermentation. The most dominant substance was isoamyl alcohol with a concentration of 1267 mg−1.The results of part 2 led to a comparison of the overall profile of volatiles between the varieties. The results showed that the condensates have both a high content of volatile substances and of alcohol. It was also shown that the Sauvignon blanc variant had the highest number of volatile compounds in the representation. The Merlot and Riesling variants were very similar. This product has an exceptionally high potential for further use in the wine or food industry.
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对葡萄汁发酵过程中收集的冷凝物的研究
本文对葡萄汁发酵过程中发酵气体形成的冷凝物进行了分析。实验分为两部分。第一部分,在雷司令葡萄的整个发酵过程中监测各种挥发物的变化。第二部分研究了三种不同葡萄品种(雷司令、梅洛和长相思)的冷凝物,并在发酵结束时测量了所选挥发性物质的总含量。重点使用气相色谱-质谱仪(GC-MS)测量了以下挥发性物质:异戊醇、异丁醇、1-丙醇、乙酸乙酯、己酸乙酯、辛酸乙酯、癸酸乙酯、乙醛、乙酸和乙醛苷。此外,还分析了发酵阶段冷凝物中酒精含量的变化。根据第一部分的结果,确定了发酵过程中产生的研究物质的数量。在发酵的第四天和第五天,风味化合物的浓度最高。最主要的物质是异戊醇,浓度为 1267 毫克-1。结果表明,冷凝物中的挥发性物质和酒精含量都很高。此外,长相思品种中的挥发性化合物数量最多。梅洛和雷司令则非常相似。该产品具有极高的潜力,可进一步用于葡萄酒或食品行业。
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