BIOMASS PRODUCTION AND SINGLE-CELL PROTEIN (SCP) ENCAPSULATION OF BACTERIA Bacillus cereus SN7

Ibnu Mukramin, F. Feliatra, Afrizal Tanjung
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Abstract

Bacillus cereus SN7 is a bacterium with potential applications in producing Single-Cell Protein (SCP). SCP can be an alternative to protein derived from traditional sources like agriculture, fisheries, and animal husbandry. Encapsulation is a process used to stabilize cells, aiming to potentially enhance probiotics' viability and stability throughout production, storage, and handling. This study aims to make capsules from B. cereus SN7 single-cell protein to protect them from contaminants. This study used a survey method, which carried out in-situ processing of bacteria, as well as conducting experiments on the formation of bacterial encapsulation, testing the effectiveness of encapsulation, and observing the viability of B. cereus SN7 bacteria by counting the number of colonies. B. cereus produced biomass with a total dry weight of 1.3708 g/mL. The alginate encapsulation experiments can be formed into capsule beads that protect bacteria. The best encapsulation process is an alginate-based coating, which is indicated by the ability of the alginate to withstand temperature, pH, and salinity
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蜡样芽孢杆菌 SN7 的生物质生产和单细胞蛋白 (SCP) 凝聚
蜡样芽孢杆菌 SN7 是一种可用于生产单细胞蛋白质(SCP)的细菌。SCP 可以替代从农业、渔业和畜牧业等传统来源获取的蛋白质。封装是一种用于稳定细胞的工艺,旨在提高益生菌在整个生产、储存和处理过程中的活力和稳定性。本研究旨在将蜡样芽孢杆菌 SN7 单细胞蛋白制成胶囊,以保护其不受污染物影响。本研究采用了一种调查方法,即对细菌进行原位处理,并对细菌封装的形成进行实验,测试封装的有效性,以及通过计数菌落数观察 B. cereus SN7 细菌的活力。B. cereus 产生的生物量总干重为 1.3708 克/毫升。海藻酸盐封装实验可形成保护细菌的胶囊珠。最好的封装工艺是基于海藻酸盐的涂层,这体现在海藻酸盐能够耐受温度、pH 值和盐度
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