Roles of extracellular polymeric substances in arsenic accumulation and detoxification by cell wall intact and mutant strains of Chlamydomonas reinhardtii

IF 5.9 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Journal of Environmental Sciences-china Pub Date : 2024-04-04 DOI:10.1016/j.jes.2024.03.048
Sadiq Naveed , Qingnan Yu , Katarzyna Szewczuk-Karpisz , Chunhua Zhang , Shafeeq-Ur Rahman , Ying Ge
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Abstract

Arsenic (As) pollution seriously threatens human and ecological health. Microalgal cell wall and extracellular polymeric substances (EPS) are known to interact with As, but their roles in the As resistance, accumulation and speciation in microalgae remain unclear. Here, we used two strains of Chlamydomonas reinhardtii, namely CC-125 (wild type) and CC-503 (cell wall-deficient mutant), to examine the algal growth, EPS synthesis, As adsorption, absorption and transformation under 10–1000 µg/L As(III) and As(V) treatments for 96 h. In both strains, the As absorption increased after the EPS removal, but the growth, As adsorption, and transformation of C. reinhardtii declined. The CC-125 strain was more tolerant to As stress and more efficient in EPS production, As accumulation, and redox transformation than CC-503, irrespective of EPS presence or absence. Three-dimension excitation-emission matrix (3D-EEM) and attenuated total reflectance infrared spectroscopy (ATR-IR) analyses showed that As was bound with functional groups in the EPS and cell wall, such as -COOH, NH and -OH in proteins, polysaccharides and amino acids. Together, this study demonstrated that EPS and cell wall acted as barriers to lower the As uptake by C. reinhardtii. However, the cell wall mutant strain was more susceptible to As toxicity due to lower EPS induction and higher As absorption.

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细胞外聚合物物质在完整细胞壁和突变株的砷积累和解毒过程中的作用
砷(As)污染严重威胁人类和生态健康。众所周知,微藻细胞壁和细胞外高分子物质(EPS)可与砷相互作用,但它们在微藻抗砷、砷积累和砷分化中的作用仍不清楚。在此,我们利用两株莱茵衣藻,即 CC-125(野生型)和 CC-503(细胞壁缺陷突变体),研究了在 10-1000 µg/L As(III) 和 As(V) 处理 96 小时后,藻体的生长、EPS 合成、As 吸附、吸收和转化情况。与CC-503相比,无论是否存在EPS,CC-125菌株对As胁迫的耐受性更强,EPS产生、As积累和氧化还原转化的效率更高。三维激发-发射矩阵(3D-EEM)和衰减全反射红外光谱(ATR-IR)分析表明,As 与 EPS 和细胞壁中的功能基团结合,如蛋白质、多糖和氨基酸中的 -COOH、NH 和 -OH。这项研究综合证明,EPS 和细胞壁是降低 C. reinhardtii 对 As 吸收的屏障。然而,细胞壁突变菌株更容易受到As毒性的影响,因为其EPS诱导率更低,As吸收率更高。
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来源期刊
Journal of Environmental Sciences-china
Journal of Environmental Sciences-china 环境科学-环境科学
CiteScore
13.70
自引率
0.00%
发文量
6354
审稿时长
2.6 months
期刊介绍: The Journal of Environmental Sciences is an international journal started in 1989. The journal is devoted to publish original, peer-reviewed research papers on main aspects of environmental sciences, such as environmental chemistry, environmental biology, ecology, geosciences and environmental physics. Appropriate subjects include basic and applied research on atmospheric, terrestrial and aquatic environments, pollution control and abatement technology, conservation of natural resources, environmental health and toxicology. Announcements of international environmental science meetings and other recent information are also included.
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