{"title":"Application of CRISPR/Cas12a for SARS-CoV-2 Nucleic Acid Detection","authors":"Xin You, Rongrong He, Suhui Li, Yibo Zhang","doi":"10.4103/mtsm.mtsm_14_23","DOIUrl":null,"url":null,"abstract":"SARS-CoV-2 spans 3 years, and although the virus has been adjusted from \"Category B, Tube A\" to \"Category B, Tube B\" it is still present in the environment, causing widespread epidemic infections and great impact on human health and the environment. Currently, there are various nucleic acid detection methods for this virus, and CRISPR methods are widely used to construct biosensors for accurate and rapid specific detection of nucleic acid sequences due to their powerful gene-editing capabilities. In this article, we introduce the application of different forms of sensors such as fluorescent paper strips built with CRISPR/Cas12a for SARS-CoV-2 nucleic acid detection.","PeriodicalId":32519,"journal":{"name":"Matrix Science Medica","volume":"93 8","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Matrix Science Medica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/mtsm.mtsm_14_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
SARS-CoV-2 spans 3 years, and although the virus has been adjusted from "Category B, Tube A" to "Category B, Tube B" it is still present in the environment, causing widespread epidemic infections and great impact on human health and the environment. Currently, there are various nucleic acid detection methods for this virus, and CRISPR methods are widely used to construct biosensors for accurate and rapid specific detection of nucleic acid sequences due to their powerful gene-editing capabilities. In this article, we introduce the application of different forms of sensors such as fluorescent paper strips built with CRISPR/Cas12a for SARS-CoV-2 nucleic acid detection.
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