Comparación de medios de cultivo para la detección de Legionella spp. en muestras de agua sanitaria: adaptación a la norma ISO 11731:2017

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES Enfermedades infecciosas y microbiologia clinica Pub Date : 2024-08-01 DOI:10.1016/j.eimc.2024.03.013

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Abstract

Introduction

Water sample culturing is the reference method to detect Legionella spp. in sanitary facilities. Until 2017, UNE-EN ISO 11731 only included the GVPC medium, which inhibits interfering microbiota but hinders the growth of Legionella spp. To improve its recovery, the new standard incorporates the BCYE medium into the working protocol.

Methods

We inoculated 1,306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of Legionella spp. detected in both media.

Results

The median in BCYE was 2,000 cfu/L higher than in GVPC (p = 0.000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence or low interfering microbiota BCYE was four times more sensitive than GVPC (p = 0.000).

Conclusion

Including BCYE in the analysis of sanitary water significantly improves the recovery of Legionella spp. in low contaminated samples.

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用于检测卫生水样中军团菌属的培养基比较：ISO 11731:2017 适应版

引言水样培养是检测卫生设施中军团菌属的参考方法。在 2017 年之前，UNE-EN ISO 11731 仅包括 GVPC 培养基，该培养基可抑制干扰微生物群，但阻碍军团菌的生长。为了提高其回收率，新标准将 BCYE 培养基纳入了工作方案。结果 BCYE 中的中位数比 GVPC 中高 2000 cfu/L（p = 0.000）。在存在大量干扰微生物群的情况下，两种培养基的灵敏度相似；在没有干扰微生物群或干扰微生物群较少的情况下，BCYE 的灵敏度是 GVPC 的四倍（p = 0.000）。

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来源期刊

Enfermedades infecciosas y microbiologia clinica 医学-传染病学

CiteScore

2.10

自引率

8.00%

发文量

194

审稿时长

29 days

期刊介绍： Hoy está universalmente reconocida la renovada y creciente importancia de la patología infecciosa: aparición de nuevos agentes patógenos, de cepas resistentes, de procesos con expresión clínica hasta ahora desconocida, de cuadros de una gran complejidad. Paralelamente, la Microbiología y la Infectología Clínicas han experimentado un gran desarrollo como respuesta al reto planteado por la actual patología infecciosa. Enfermedades Infecciosas y Microbiología Clínica es la Publicación Oficial de la Sociedad Española SEIMC. Cumple con la garantía científica de esta Sociedad, la doble función de difundir trabajos de investigación, tanto clínicos como microbiológicos, referidos a la patología infecciosa, y contribuye a la formación continuada de los interesados en aquella patología mediante artículos orientados a ese fin y elaborados por autores de la mayor calificación invitados por la revista.