Identification of a key signaling network regulating perennating bud dormancy in Panax ginseng

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL Journal of Ginseng Research Pub Date : 2024-04-23 DOI:10.1016/j.jgr.2024.04.004
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Abstract

Background

The cycle of seasonal dormancy of perennating buds is an essential adaptation of perennial plants to unfavorable winter conditions. Plant hormones are key regulators of this critical biological process, which is intricately connected with diverse internal and external factors. Recently, global warming has increased the frequency of aberrant temperature events that negatively affect the dormancy cycle of perennials. Although many studies have been conducted on the perennating organs of Panax ginseng, the molecular aspects of bud dormancy in this species remain largely unknown.

Methods

In this study, the molecular physiological responses of three P. ginseng cultivars with different dormancy break phenotypes in the spring were dissected using comparative genome-wide RNA-seq and network analyses. These analyses identified a key role for abscisic acid (ABA) activity in the regulation of bud dormancy. Gene set enrichment analysis revealed that a transcriptional network comprising stress-related hormone responses made a major contribution to the maintenance of dormancy.

Results

Increased expression levels of cold response and photosynthesis-related genes were associated with the transition from dormancy to active growth in perennating buds. Finally, the expression patterns of genes encoding ABA transporters, receptors (PYRs/PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs), and DELLAs were highly correlated with different dormancy states in three P. ginseng cultivars.

Conclusion

This study provides evidence that ABA and stress signaling outputs are intricately connected with a key signaling network to regulate bud dormancy under seasonal conditions in the perennial plant P. ginseng.

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人参常年芽休眠的关键信号网络的鉴定
背景常年花芽的季节性休眠周期是多年生植物对冬季不利条件的一种基本适应。植物激素是这一关键生物过程的关键调节因子,而这一过程又与各种内外因素密切相关。最近,全球变暖增加了异常温度事件的频率,对多年生植物的休眠周期产生了负面影响。本研究利用全基因组 RNA-seq 比较和网络分析方法,剖析了三个具有不同春季休眠表型的人参栽培品种的分子生理反应。这些分析确定了脱落酸(ABA)活性在芽休眠调控中的关键作用。基因组富集分析表明,由胁迫相关激素反应组成的转录网络对维持休眠做出了重要贡献。结果冷反应和光合作用相关基因表达水平的增加与常年芽从休眠向活跃生长的过渡有关。最后,编码 ABA 转运体、受体(PYRs/PYLs)、蛋白磷酸酶 2Cs (PP2Cs)和 DELLAs 的基因的表达模式与三个人参栽培品种的不同休眠状态高度相关。
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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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