The Influence of Chitosan on the Ability of LPS to Interact with Cells of the Immune System

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI:10.1134/S0003683824020029

V. N. Davydova, A. V. Volod’ko, V. I. Gorbach, S. V. Chusovitina, T. F. Solov’eva, I. M. Yermak

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Abstract

Complexes of lipopolysaccharide (LPS) from the bacterium Escherichia coli and chitosan (CN) with a molecular weight of 5 kDa were obtained and their supramolecular organization was studied. Using atomic force microscopy, it was shown that during the formation of complexes there is a transition from the micellar structure of the original LPS to linear network structures uniformly distributed over the surface of mica. The stability of LPS-CN complexes of various stoichiometries in biological media in the presence of serum proteins was investigated. It was shown that complexes with an LPS–CN ratio of 1 : 1 in the presence of serum proteins lost their surface charge and tended to aggregate while complexes with the maximum saturation of CN (1 : 5) did not aggregate under these conditions and maintained their surface charge. The effect of CNs of different molecular weights on the ability of LPS to interact with neutrophils of human whole blood was studied. It was observed that LPS-CN complexes were capable of binding to neutrophils and internalazing the cell, and this ability was enhanced in the presence of serum proteins. Chitosan exhibited the ability to suppress the synthesis of the proinflammatory cytokine TNF-α induced by LPS, not only as part of the complex but also when cells were pretreated with a polycation.

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壳聚糖对 LPS 与免疫系统细胞相互作用能力的影响

研究人员获得了分子量为 5 kDa 的大肠杆菌脂多糖（LPS）与壳聚糖（CN）的复合物，并对其超分子组织结构进行了研究。原子力显微镜显示，在形成复合物的过程中，原始 LPS 的胶束结构转变为均匀分布在云母表面的线性网络结构。研究还考察了不同化学计量的 LPS-CN 复合物在有血清蛋白存在的生物介质中的稳定性。结果表明，在有血清蛋白存在的情况下，LPS-CN 比率为 1 : 1 的复合物会失去其表面电荷并趋于聚集，而 CN 饱和度最大（1 : 5）的复合物在这些条件下不会聚集并保持其表面电荷。研究了不同分子量的氯化萘对 LPS 与人体全血中性粒细胞相互作用能力的影响。结果表明，LPS-CN 复合物能够与中性粒细胞结合并内凝细胞，在有血清蛋白存在的情况下，这种能力会增强。壳聚糖能够抑制 LPS 诱导的促炎细胞因子 TNF-α 的合成，不仅是作为复合物的一部分，而且在用聚阳离子预处理细胞时也是如此。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.