Syndecan-1 As a Potential Messenger of Remote Postconditioning Effects in Experiments with Brain Ischemia

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Evolutionary Biochemistry and Physiology Pub Date : 2024-04-26 DOI:10.1134/s0022093024020170

M. E. Kolpakova, A. A. Jakovleva, L. S. Polyakova, H. El Amghari, S. Soliman, D. R. Faizullina, V. V. Sharoiko

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Abstract

The mechanisms of cerebral ischemia–reperfusion injury (IRI) limitation by remote ischemic postconditioning (RPC) are of interest because the latter may positively influence functional recovery after cerebral ischemia. The study was aimed to assess the role of plasma proteins syndecan-1 (SDC-1) and annexin-5 (ANXA-5) in limiting cerebral IRI by RPC in a middle cerebral artery occlusion (MCAO) model in male Wistar rats. The animals were randomized into three groups: (1) control sham-operated rats (SO group), (2) rats with 30-min MCAO-induced ischemia (MCAO group), (3) rats with 30-min MCAO-induced ischemia followed by RPC (MCAO + RPC group). SDC-1 plasma levels, as assessed by ELISA, in MCAO (41.4 ± 1.3 ng/mL) and MCAO + RPC (67.8 ± 5.8 ng/mL) rats were, respectively, by 30% (p < 0.05) and 112% (p < 0.01) higher than in SO control (31.9 ± 1.1 ng/mL). No intergroup differences in ANXA-5 plasma levels were detected. Cerebral infarct volume in MCAO and MCAO + RPC rats accounted for 32.0 ± 2.5 and 13.6 ± 1.3% of the total brain volume, respectively (p < 0.05). Cerebral edema volume in MCAO and MCAO + RPC rats were 47.0 ± 3.3 and 16.0 ± 2.1%, respectively (p < 0.05). In MCAO + RPC animals, correlation analysis revealed a high direct correlation between infarct size and right forelimb muscular strength (grip strength test, 3.9 ± 0.8 H, p > 0.5; KK = 0.72, p < 0.05) and a very high inverse correlation between infarct size and right hindlimb capillary blood flow (Doppler flowmetry, r = –0.98, p < 0.01). Animals’ neurologic deficits were assessed by the Garcia scoring scale, while their locomotor coordination was assessed in the Rotarod test. It is assumed that SDC-1 plasma protein may serve as a potential regulator of the infarct size-limiting effect of remote ischemic postconditioning, which is determinative for functional recovery.

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在脑缺血实验中，Syndecan-1 是远程后处理效应的潜在信使

摘要远程缺血后条件（RPC）限制脑缺血再灌注损伤（IRI）的机制令人感兴趣，因为后者可能对脑缺血后的功能恢复产生积极影响。本研究旨在评估在雄性 Wistar 大鼠大脑中动脉闭塞（MCAO）模型中，质粒蛋白辛迪加-1（SDC-1）和附件蛋白-5（ANXA-5）通过 RPC 限制脑缺血后损伤（IRI）的作用。动物随机分为三组：（1）假手术对照组（SO 组）；（2）30 分钟 MCAO 诱导缺血组（MCAO 组）；（3）30 分钟 MCAO 诱导缺血后 RPC 组（MCAO + RPC 组）。经 ELISA 评估，MCAO 组（41.4 ± 1.3 ng/mL）和 MCAO + RPC 组（67.8 ± 5.8 ng/mL）大鼠的 SDC-1 血浆水平分别比 SO 对照组（31.9 ± 1.1 ng/mL）高 30% (p < 0.05) 和 112% (p < 0.01)。未发现 ANXA-5 血浆水平的组间差异。MCAO 和 MCAO + RPC 大鼠的脑梗塞体积分别占脑总体积的 32.0 ± 2.5% 和 13.6 ± 1.3% (p <0.05)，MCAO 和 MCAO + RPC 大鼠的脑水肿体积分别为 47.0 ± 3.3% 和 16.0 ± 2.1% (p <0.05)。在 MCAO + RPC 动物中，相关性分析显示梗死大小与右前肢肌肉强度之间存在高度直接相关性（握力测试，3.9 ± 0.8 H，p >0.5；KK = 0.72，p <0.05），梗死大小与右后肢毛细血管血流量之间存在非常高的反相关性（多普勒血流测量，r = -0.98，p <0.01）。动物的神经功能缺损通过加西亚评分量表进行评估，而运动协调性则通过旋转木马测试进行评估。据推测，SDC-1血浆蛋白可能是远端缺血后条件下限制梗死面积效应的潜在调节因子，而远端缺血后条件对功能恢复起决定性作用。

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来源期刊

Journal of Evolutionary Biochemistry and Physiology 生物-进化生物学

自引率

33.30%

发文量

110

审稿时长

6-12 weeks

期刊介绍： Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.