Expression of Molecules Characterizing Metabolic and Cytotoxic Activity of Different Natural Killer Cell Subpopulations in Peripheral Blood during Pregnancy
E. G. Orlova, O. A. Loginova, O. L. Gorbunova, S. V. Shirshev
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引用次数: 0
Abstract
The functions of peripheral blood natural killer (NK) cells
change significantly throughout pregnancy, which is mainly due to
the inhibition of their cytotoxicity. Although the functional activity
of NK cells is directly related to their metabolic status, these
changes during physiological pregnancy have not been studied. The
aim of this work was to study the expression of Glut-1, CD94, and
CD107a molecules characterizing metabolic and cytotoxic activity,
as well as the mitochondrial mass, in different peripheral blood
NK cell subpopulations of healthy women during first and third trimesters
of physiological pregnancy. The control group comprised healthy
non-pregnant women in the follicular phase of the menstrual cycle.
The expression of Glut-1, CD94, CD107a molecules and the mitochondrial
mass were analyzed by flow cytometry in regulatory (CD16–CD56bright),
cytotoxic (CD16+CD56dim),
and minor cytotoxic (CD16hiCD56–)
NK cells. It was found that in non-pregnant women, minor cytotoxic CD16hiCD56– NK
cells had the highest expression of Glut-1 and CD107a and the lowest
expression of CD94 compared to other NK cell subpopulations. In
regulatory CD16–CD56bright and
cytotoxic CD16+CD56dim NK
cells, the expression of these molecules was similar, as was the
mitochondrial mass in all subpopulations studied. In the first trimester,
Glut-1 expression increased on regulatory CD16–CD56bright NK
cells, while mitochondrial mass and the expression of CD94 and CD107a
on all NK cells did not differ from those in non-pregnant women.
In the third trimester, mitochondrial mass increased, but CD94 expression
decreased, in cytotoxic CD16+CD56dim NK
cells compared to non-pregnant women, while CD94 expression on regulatory
CD16–CD56bright NK
cells was higher than in the first trimester. CD107a expression
on minor cytotoxic CD16hiCD56– NK
cells decreased, while remaining unchanged in other subpopulations
compared to non-pregnant women. Glut-1 expression did not change
in all subpopulations. Thus, different subpopulations of peripheral
blood NK cells are heterogeneous in the expression of Glut-1, CD107a,
and CD94. The expression of these molecules during physiological
pregnancy varies by trimesters. The obtained results are important
for understanding the regulatory mechanisms of NK cell functioning
during pregnancy.
摘要 外周血自然杀伤细胞（NK）的功能在整个妊娠期间发生显著变化，这主要是由于其细胞毒性受到抑制。虽然 NK 细胞的功能活性与其新陈代谢状态直接相关，但在生理妊娠期间这些变化尚未被研究过。这项工作的目的是研究健康女性在生理性妊娠的前三个月和后三个月中不同外周血 NK 细胞亚群中 Glut-1、CD94 和 CD107a 分子的表达情况，这些分子表征了 NK 细胞的代谢和细胞毒性活性以及线粒体质量。流式细胞术分析了调节性（CD16-CD56bright）、细胞毒性（CD16+CD56dim）和轻微细胞毒性（CD16hiCD56-）NK 细胞中 Glut-1、CD94、CD107a 分子的表达和线粒体质量。研究发现，与其他 NK 细胞亚群相比，在非妊娠妇女中，CD16hiCD56-小细胞毒性 NK 细胞的 Glut-1 和 CD107a 表达量最高，CD94 表达量最低。在调节性 CD16-CD56bright 和细胞毒性 CD16+CD56dim NK 细胞中，这些分子的表达相似，在所有研究的亚群中线粒体质量也相似。在妊娠头三个月，调节性 CD16-CD56bright NK 细胞中 Glut-1 的表达增加，而线粒体质量以及所有 NK 细胞中 CD94 和 CD107a 的表达与非妊娠妇女没有差异。与非妊娠妇女相比，妊娠三个月时，细胞毒性 CD16+CD56dim NK 细胞的线粒体质量增加，但 CD94 表达量减少，而调节性 CD16-CD56bright NK 细胞的 CD94 表达量高于妊娠头三个月。与非妊娠妇女相比，CD16hiCD56-NK细胞中的CD107a表达量减少，而其他亚群的表达量保持不变。所有亚群中 Glut-1 的表达均无变化。因此，不同亚群的外周血 NK 细胞在 Glut-1、CD107a 和 CD94 的表达上存在差异。这些分子在生理妊娠期的表达因孕期而异。这些结果对了解妊娠期 NK 细胞功能的调节机制具有重要意义。
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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