The crystallization properties of antifreeze GelMA hydrogel and its application in cryopreservation of tissue-engineered skin constructs

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-04-27 DOI:10.1002/jbm.b.35408

Jia Tan, Jiahui Li, Xinli Zhou

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Abstract

Gelatin methacrylate (GelMA) hydrogels are expected to be ideal skin tissue engineering dressings for a wide range of clinical treatments. Herein, we report the preparation of GelMA or antifreeze GelMA hydrogel sheets with different GelMA concentrations, crosslinking times, and cryoprotectant (CPA) concentrations. The crystallization properties of GelMA or antifreeze GelMA hydrogel sheets were studied by cryomicroscopy and differential scanning calorimetry (DSC). It was found that the growth of ice crystals was slower when GelMA hydrogel concentration was more than 7%. The 10% DMSO-7% GelMA hydrogel sheets crosslinked for 60 min showed no ice crystal formation and growth during cooling and warming. The DSC results showed that the vitrification temperature of the 10% DMSO-7% GelMA hydrogel sheet was −111°C. Furthermore, slow freezing and rapid freezing of fibroblast-laden GelMA or antifreeze GelMA hydrogel sheets, and tissue-engineered skin constructs were studied. The results showed no significant difference in cell survival between slow (88.8% ± 1.51) and rapid (89.2% ± 3.00) freezing of fibroblast-loaded 10% DMSO-7% GelMA hydrogel sheets, and significantly higher than that of 7% GelMA hydrogel sheets (33.4% ± 5.46). The cell viability was higher in tissue-engineered skin constructs after slow freezing (86.34% ± 1.45) than rapid freezing (72.74% ± 1.34). We believe that the combination of antifreeze hydrogels and tissue engineering will facilitate the cryopreservation of tissue engineering constructs.

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抗冻 GelMA 水凝胶的结晶特性及其在组织工程皮肤构建物低温保存中的应用

甲基丙烯酸明胶（GelMA）水凝胶有望成为用于各种临床治疗的理想皮肤组织工程敷料。在此，我们报告了不同 GelMA 浓度、交联时间和低温保护剂（CPA）浓度下 GelMA 或抗冻 GelMA 水凝胶片材的制备过程。通过冷冻显微镜和差示扫描量热法（DSC）研究了 GelMA 或抗冻 GelMA 水凝胶片的结晶特性。结果发现，当 GelMA 水凝胶的浓度超过 7% 时，冰晶的生长速度较慢。交联 60 分钟的 10% DMSO-7% GelMA 水凝胶片材在冷却和升温过程中没有冰晶形成和生长。DSC 结果表明，10% DMSO-7% GelMA 水凝胶片材的玻璃化温度为 -111°C 。此外，还研究了含有成纤维细胞的 GelMA 或抗冻 GelMA 水凝胶片材以及组织工程皮肤构建物的缓慢冷冻和快速冷冻。结果表明，缓慢冷冻（88.8% ± 1.51）和快速冷冻（89.2% ± 3.00）含成纤维细胞的 10% DMSO-7% GelMA 水凝胶片材的细胞存活率无明显差异，但显著高于含 7% GelMA 水凝胶片材的细胞存活率（33.4% ± 5.46）。慢速冷冻（86.34% ± 1.45）后组织工程皮肤构建体的细胞存活率高于快速冷冻（72.74% ± 1.34）。我们相信，防冻水凝胶与组织工程的结合将有助于组织工程构建物的冷冻保存。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part B, Applied biomaterials 工程技术-材料科学：生物材料

CiteScore

7.50

自引率

2.90%

发文量

199

审稿时长

12 months

期刊介绍： Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.

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